Mr. T. H. Pope

Abstract

future be surpassing highbrow and surpassing happy!" The following sign poem was then delivered by Mr. Tailer, with musical accompaniment by the lecturer: "Were I in future blind, yet would I always have A rainbow in my life, since you love me. My red would be your lips, yellow your golden hair-Your fragrance violet, and green scented leaves. My blue would be your eyes, your arms would light my heart. Your absence be my gloom, your soul-my sun! "

Cross‐linking of collagen CNBr peptides by ozone or UV light

“…Since the finding of blue fluorescence in uterine collagen [8], age-related blue 420-460 nm fluorescence has been reported in collagens from various tissues [9-141. Another 395 nm fluorescence, due to pyridinoline, is present in hard tissue collagens (achilles tendon, cartilage, bone, dentin), but not in soft tissue collagens (skin, cornea, tail tendon) [15,16]. This study shows the presence of both 430 nm blue fluorescence and the novel 360-370 nm fluorescence in CNBr peptides from pyridinoline-free rat tail tendon collagen.…”

Collagen cross-linking in human bone and articular cartilage. Age-related changes in the content of mature hydroxypyridinium residues

“…A direct h.p.l.c./fluorescence assay (Eyre et al, 1984b) was used to measure the two forms of hydroxypyridinium cross-link (HP and LP) in all samples of bone and cartilage. This method could be applied directly to hydrolysates of crude tissue, including mineralized bone, without an intermediate clean-up step by molecular sieve chromatography.…”

“…to measure hydroxypyridinium cross-linking residues directly in hydrolysates of human bone and cartilage Dried samples (10 mg) of cartilage and mineralized bone were hydrolysed in 6 M-HCl for 24 h at 108 'C. The dried hydrolysates were redissolved in 1 % (v/v) n-heptafluorobutyric acid and chromatographed on a reverse-phase C18 column [Beckman (Altex) Ultrasphere ODS; 25 cm x 4.6 mm), eluted with a gradient of acetonitrile in 0.01 M-heptafluorobutyric acid as previously described (Eyre et al, 1984b): (a) 30,g of hydrolysed cartilage (15 #sg of collagen); (b) 775 ,ug of hydrolysed bone (150 ,ug of collagen). Fluorescence was determined with excitation at 297 nm and emission at > 380 nm.…”