MR Imaging of in Vivo Recruitment of Iron Oxide–labeled Macrophages in Experimentally Induced Soft-Tissue Infection in Mice

Lee, Jin Seong; Kang, Hee Jung; Gong, Gyungyub; Jung, Heun-Don; Lim, Keun Ho; Lim, Tae‐Hwan

doi:10.1148/radiol.2403051156

Cited by 22 publications

(23 citation statements)

References 14 publications

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“…Injection of 3×10 7 cells resulted in a significant SI decline in all investigated target organs, i.e., liver, spleen, and bone marrow. We previously showed that injected macrophages were recruited to the area of soft tissue infection and positioned in the wall of the abscess [8], a finding confirmed here.…”

Section: Discussionsupporting

confidence: 89%

“…Owing to the presence of iron oxide in the macrophages, the wall of the abscess showed a susceptibility effect on T2*-weighted MR images. The lower SI band of the abscess wall correlated with the distribution of iron-oxide-labeled macrophages in histopathological specimens [8].…”

Section: Discussionmentioning

confidence: 76%

“…Recently, optimized labeling techniques for superparamagnetic iron oxide (SPIO) have improved the efficiency of macrophage labeling and have allowed the ex vivo labeling of cells with contrast [7]. Previously, with the ex vivo labeling technique, we have demonstrated the feasibility of MR imaging using a 4.7-T magnetic field to assess the recruitment of macrophages in soft tissue infection [8].…”

Section: Introductionmentioning

confidence: 99%

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Pace of macrophage recruitment during different stages of soft tissue infection: Semi-quantitative evaluation by in vivo magnetic resonance imaging

et al. 2008

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We describe the pace of recruitment of iron-oxide-labeled macrophages to the site of different stages of infection by in vivo magnetic resonance (MR) imaging. Peritoneal macrophages were labeled with superparamagnetic iron oxide ex vivo and administered through the tail vein 6 (acute) or 48 (subacute) h after bacterial inoculation. The legs of the mice were imaged sequentially on a 4.7-T MR unit before and 3, 6, 12, 18, 24, 48 and 72 h after macrophage administration. The band-shaped lower signal intensity zone around the abscess on T2*-weighted GRE images became more obvious due to recruited macrophages up until 24 h after injection in the subacute and 48 h after injection in the acute group, indicating that the relative SI of the abscess wall decreased more rapidly and the pace of recruitment of macrophages was faster in the subacute than in the acute group. Chemokine antibody arrays of mouse sera detected increased concentration of granulocyte-colony-stimulating factor and tissue inhibitor of metalloproteinase-1 beginning at 12 h and increased interleukin-13 at 18 h. Monocyte chemoattractant protein-1 and macrophage-colony-stimulating factor began to increase at 96 h after infection. This difference in pace of recruitment may result from the release of chemokines.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 76%

Section: Introductionmentioning

confidence: 99%

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Pace of macrophage recruitment during different stages of soft tissue infection: Semi-quantitative evaluation by in vivo magnetic resonance imaging

et al. 2008

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“…Advances in molecular imaging have further enabled the use of non-invasive, real-time monitoring techniques for cell migration in vivo, and magnetic resonance imaging (MRI) plays an important role in both pre-clinical and clinical studies. For these types of studies, iron oxide-based nanoparticles with magnetic properties can be used for cell labeling (5)(6)(7) . MRI provides detailed information on anatomy and function, and this technique is commonly used for cell tracking assays in vivo in combination with contrast agents, such as superparamagnetic iron oxide nanoparticles (SPIONs).…”

Section: Introductionmentioning

confidence: 99%

In vivo magnetic resonance imaging tracking of C6 glioma cells labeled with superparamagnetic iron oxide nanoparticles

Mamani

Malheiros

Cardoso

et al. 2012

Einstein (São Paulo)

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Objective: The aim of the current study was to monitor the migration of superparamagnetic iron oxide nanoparticle (SPION)-labeled C6 cells, which were used to induce glioblastoma tumor growth in an animal model, over time using magnetic resonance imaging (MRI), with the goal of aiding in tumor prognosis and therapy. Methods: Two groups of male Wistar rats were used for the tumor induction model. In the first group (n=3), the tumors were induced via the injection of SPION-labeled C6 cells. In the second group (n=3), the tumors were induced via the injection of unlabeled C6 cells. Prussian Blue staining was performed to analyze the SPION distribution within the C6 cells in vitro. Tumor-inducing C6 cells were injected into the right frontal cortex, and subsequent tumor monitoring and SPION detection were performed using T 2 -and T 2 *-weighted MRI at a 2T field strength. In addition, cancerous tissue was histologically analyzed after performing the MRI studies. Results: The in vitro qualitative evaluation demonstrated adequate distribution and satisfactory cell labeling of the SPIONs. At 14 or 21 days after C6 injection, a SPIONinduced T 2 -and T 2 *-weighted MRI signal reduction was observed within the lesion located in the left frontal lobe on parasagittal topography. Moreover, histological staining of the tumor tissue with Prussian Blue revealed a broad distribution of SPIONs within the C6 cells. Conclusion: MRI analyses exhibit potential for monitoring the tumor growth of C6 cells efficiently labeled with SPIONs.

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“…Indeed, inflammation conditions are often associated with enhanced permeability [3], thus resulting in interstitial USPIO accumulation not necessarily directly related to the cellular inflammatory response [4]. Alternative cellular MRI techniques involve administration of ex vivo magnetically labelled cells and subsequent tracking of their trafficking in inflamed tissues [5,6]. While the low cellular uptake of USPIOs is an advantage for in vivo labelling (because of the resulting long vascular remanence) [7,8], this property makes them ill suited for in vitro labelling.…”

Section: Introductionmentioning

confidence: 99%

Quantitative effects of cell internalization of two types of ultrasmall superparamagnetic iron oxide nanoparticles at 4.7 T and 7 T

Brisset

Desestret²,

Marcellino³

et al. 2009

Eur Radiol

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MR Imaging of in Vivo Recruitment of Iron Oxide–labeled Macrophages in Experimentally Induced Soft-Tissue Infection in Mice

Abstract: Homing of intravenously administered iron oxide-labeled macrophages can be monitored with MR imaging and may provide a tool to investigate interactions between macrophages and the invading pathogens.

Cited by 22 publications

References 14 publications

Pace of macrophage recruitment during different stages of soft tissue infection: Semi-quantitative evaluation by in vivo magnetic resonance imaging

Pace of macrophage recruitment during different stages of soft tissue infection: Semi-quantitative evaluation by in vivo magnetic resonance imaging

In vivo magnetic resonance imaging tracking of C6 glioma cells labeled with superparamagnetic iron oxide nanoparticles

Quantitative effects of cell internalization of two types of ultrasmall superparamagnetic iron oxide nanoparticles at 4.7 T and 7 T

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