“…Among these are centromeric proteins Cse4, Cep3, Ndc10 in yeast, and CENPI [35,40,41], the helicase PICH [42], and the centromere-associated kinase Aurora B/AURKB along with its partners Borealin/CDCA8, MIS18BP1 and Survivin/BIRC5 (Bir1 in S. cerevisiae ) in mammalian cells [35,43,44,45,46]. At the level of the outer kinetochore, one finds SUMOylated Ndc80 and NUF2 [35,36], as well as the kinesins CENPE/KIF10 [36] and KIF18A [47], the RNA binding protein NKAP [48], the Ran GTPase activating protein RanGAP1 [49], the ANAPC4 subunit of the APC/C [50,51], along with the protein kinases BUB1B/BubR1 [52] and MPS1/TTK [53]. When known, most of these proteins are SUMOylated by SUMO2/3, and often require PIAS4 (also known as PIASγ) as a E3 SUMO ligase (see Table 1).…”