Mp34-10 the Effect of Insulin Use in Type Ii Diabetes on 24 Hour Urine Parameters

Abstract

METHODS: Methods: Diet-induced obese male rats underwent RYGB (n¼8) or sham (n¼7) surgery with pre-and post-operative urine and stool collections at specified time-points. At 8 weeks post-surgery, all animals were placed on high-calcium diet (2.4%) for 2 weeks and then returned to normal calcium diet (0.6%) for 2 weeks. Rats were then orally gavaged with an actively growing, 24-hour pure culture of a wild, rat-colonizing strain of O.f. (35 mg of wet weight) and followed for 7 weeks. Alterations in intestinal microbiota were analyzed using 16S rRNA Illumina paired-end sequencing followed by UniFrac principal component analysis and linear discriminant analysis coupled with effect sized (LEfSe) measurements.RESULTS: Results: Gut microbial community structure was significantly different in RYGB animals compared to sham controls. Manipulation in dietary calcium had minimal effects on the gut microbiome structure. Oral gavage with O.f. induced a modest change in the overall microbial community structure in RYGB animals but not in sham animals. Interestingly, O.f. treatment increased the abundance of Bifidobacterium and Bacteroidales in RYGB animals, and reduced their urinary oxalate levels by 75%. The abundance of these species was not increased in sham animals.CONCLUSIONS: Conclusions: The RYGB procedure significantly altered the gut microbiome compared to controls. O.f. colonization but not dietary calcium manipulations in RYGB animals induced a modest change in the composition and structure of the intestinal microbiome. In contrast, the intestinal microbiota of sham animals was stable upon O.f. challenge. These data suggest that O.f. may serve as a keystone species in altering the intestinal microbial community in RYGB animals and warrant further studies in human RYGB patients.