Mp10-04 the Protective Roles of Estrogen Receptor Β in Renal Calcium Oxalate Crystal Formation via Reducing the Liver Oxalate Biosynthesis and Renal Oxidative Stress-Mediated Cell Injury

Zhu, Wei; Zhang, Huaru; Bushinsky, David A.; Chang, Chawnshang; Zeng, Guohua; Yeh, Shuyuan

doi:10.1097/ju.0000000000000830.04

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“…Moreover, the release of proapoptotic factors from the mitochondria with lost membrane potential causes apoptosis and leads to kidney damage and the occurrence of calculi [61]. Zhu et al [78] studied the estrogen receptor β and found that it can inhibit the formation of CaOx crystals by reducing oxalate biosynthesis in the liver and cell damage mediated by renal oxidative stress. Therefore, our results demonstrated the potential of CDSPs to inhibit oxidative stress and prevent gallstones.…”

Section: Repair Of Damaged Hk-2 Cells By Cdspsmentioning

confidence: 99%

Carboxymethylation of Desmodium styracifolium Polysaccharide and Its Repair Effect on Damaged HK-2 Cells

Tang

Liu

Sun

et al. 2022

Oxidative Medicine and Cellular Longevity

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Objective. Desmodium styracifolium is the best traditional medicine for treating kidney calculi in China. This study is aimed at increasing the carboxyl (-COOH) content of D. styracifolium polysaccharide (DSP0) and further increasing its antistone activity. Methods. DSP0 was carboxymethylated with chloroacetic acid at varying degrees. Then, oxalate-damaged HK-2 cells were repaired with modified polysaccharide, and the changes in biochemical indices before and after repair were detected. Results. Three modified polysaccharides with 7.45% (CDSP1), 12.2% (CDSP2), and 17.7% (CDSP3) -COOH are obtained. Compared with DSP0 (-COOH content = 1.17 % ), CDSPs have stronger antioxidant activity in vitro and can improve the vitality of damaged HK-2 cells. CDSPs repair the cell morphology and cytoskeleton, increase the cell healing ability, reduce reactive oxygen species and nitric oxide levels, increase mitochondrial membrane potential, limit autophagy level to a low level, reduce the eversion of phosphatidylserine in the cell membrane, weaken the inhibition of oxalate on DNA synthesis, restore cell cycle to normal state, promote cell proliferation, and reduce apoptosis/necrosis. Conclusion. The carboxymethylation modification of DSP0 can improve its antioxidant activity and enhance its ability to repair damaged HK-2 cells. Among them, CDSP2 with medium -COOH content has the highest activity of repairing cells, whereas CDSP3 with the highest -COOH content has the highest antioxidant activity. This difference may be related to the active environment of polysaccharide and conformation of the polysaccharide and cell signal pathway. This result suggests that Desmodium styracifolium polysaccharide with increased -COOH content may have improved potential treatment and prevention of kidney calculi.

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Section: Repair Of Damaged Hk-2 Cells By Cdspsmentioning

confidence: 99%