Mp06-13 inhibition of Human Prostate Stromal Cell Growth and Modulation of Cytoskeletal Organization by Lenalidomide: A New Treatment Option in Male Luts?

Abstract

cells cultured with rapamycin (Rap, autophagy activator) treatment for 3 days, the conditioned media was harvested and used to evaluate the proliferation of Prsc cells. Quantitative PCR was used to identify cytokines secreted by Prsc cells treated with rapamycin.RESULTS: 1.The expression of LC3 protein was overexpressed in early-progressed BPH patients than the other groups and it was positive correlation with a-smooth muscle actin and collagen I. 2. Autophagy inhibitor CQ could suppress the expression of a-smooth muscle actin and collagen I in Prsc cells via AKT and Erk signaling pathways. Furthermore, it promoted the expression of cleaved-PARP protein and p62. 3. The conditioned media (rapamycin to Prsc) could accelerate the proliferation of Prsc53 cell. 4. CCL5, CXCL8, CXCL12, CXCL13, IL-5, IL-17a and FGF6 were increased in Prsc53 cell after treated with rapamycin for 24 hours.CONCLUSIONS: Autophagy may be as an important effector in the progression of benign prostatic hyperplasia through stimulating myofibroblast phenotype, proliferation and its apoptosis.