MP-08.04

Pourmand, Gholamreza; Abedi, Amir Reza; Mehrsai, Abdolrasoul; Alavi, H; Ahmadi, Ali; Ziaee, Abed-Ali; Emamzadeh, A.

doi:10.1016/j.urology.2006.08.311

Cited by 1 publication

(1 citation statement)

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“…One of the cancer types, prostate cancer is classified as the most commonly diagnosed cancer among the men. The current prognostic tools such as preoperative prostate specific antigen (PSA) levels and histological Gleason grading of biopsy specimens are not available to determine risk stratify of individual patients with prostate cancer at early stages of the disease . The prostate cancer model by PTEN has been described for diagnosis in the initiation of prostate cancer with prostatic intraepithelial neoplasia (PIN), followed by progression to invasive adenocarcinoma, and subsequent metastasis .…”

Section: Introductionmentioning

confidence: 99%

Design of a probe based on poly(glycidyl methacrylate‐co‐vinylferrocene)‐coated Pt electrode for electrochemical detection of PTEN gene in PCR amplified samples from prostate tissues

Baş

Maltaş

Sennik

et al. 2014

J of Applied Polymer Sci

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In this study, a new probe based on immobilization of amino linked oligonucleotide (NH2‐linked DNA) on poly(glycidyl methacrylate‐co‐vinylferrocene)‐coated Pt electrode was fabricated for the electrochemical detection of PTEN gene from human prostate tissues. The experimental parameters such as DNA immobilization time, DNA concentration, and target concentration were optimized. The selectivity of the NH2‐linked DNA probe was assessed with mismatch (MM) and noncomplementary (NC) sequences. The applicability of the NH2‐linked DNA probe to the PCR amplified samples correspond to PTEN gene from prostate tissues was evaluated. The immobilization of DNA on the copolymer was confirmed by FTIR, AFM, CV and DPV analysis. The PCR products were also identified by using agarose gel electrophoresis. The prepared probe indicated a linear range (10–100 μg mL−1) with a detection limit (4.7 μg mL−1) and a good selectivity of the NH2‐linked DNA probe toward target DNA sequence. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40638.

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Section: Introductionmentioning

confidence: 99%