Moxidectin residues in lamb tissues: Development and validation of analytical method by UHPLC-MS/MS

Cruz, Michelle Del Bianchi A.; Fernandes, Maria Ângela Machado; Braga, Patrícia Aparecida de Campos; Monteiro, Alda Lúcia Gomes; Daniel, Daniela; Reyes, Felix G. R.

doi:10.1016/j.jchromb.2017.11.041

Cited by 7 publications

(3 citation statements)

References 25 publications

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“…In the modern world, all chromatographic techniques that support separations using shorter columns, faster flow rates for high speed, and better resolution and sensitivity are available through UHPLC. (30) The following table 4 lists the results of the quantitative analysis of moxidectin using UHPLC. For the chromatographic separation of the analytes in plasma, two RP UPLC columns, the BEH C18 column (50 × 2.1 mm, 1.8 μm) and the Acquity HSS-T3 column (100 mm × 2.1 mm, 1.8 μm), were assessed with mobile phased made up of 0.01% acetic acid in ACN and methanol.…”

Section: Ultra High-performance Liquid Chromatography (Uhplc)mentioning

confidence: 99%

A Review on Different Analytical Techniques for Quantification of Moxidectin

Kommu,

Sundararajan

2024

Journal of the Turkish Chemical Society Section A: Chemistry

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Nemadectin, a macrocyclic lactone of the milbemycin class, is a fermentation by-product of the bacteria Streptomyces cyanogriseus subsp. non-Cyanogenus. Moxidectin is a semi-synthetic derivative of nemadectin. River blindness, also known as onchocerciasis, is treated with moxidectin in patients 12 years of age and older. This condition is brought on by the parasitic worm Onchocerca volvulus and is subjected to intense itching, skin conditions that are disfiguring, and impaired vision brought on by the larvae of the worm. Some of the most common internal and exterior parasites are killed by moxidectin by selectively binding to their glutamate-gated chloride ion channels. In this review article, various pieces of equipment, such as a UV spectrometer, HPLC, LC-MS, and UPLC-MS, are used to determine moxidectin as well as its related compounds. The QuEChERS method was also used in the sample preparation according to the literature survey. The report also offers an overview of the pharmacodynamics, pharmacokinetics, and medication interactions of moxidectin.

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Section: Ultra High-performance Liquid Chromatography (Uhplc)mentioning

confidence: 99%

A Review on Different Analytical Techniques for Quantification of Moxidectin

Kommu,

Sundararajan

2024

Journal of the Turkish Chemical Society Section A: Chemistry

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“…Liquid chromatography (LC) combined with other discovery systems, such as UV or diode array, fluorescence or mass spectrometry (MS), is considered the best technique to quantify multiresidues of veterinary drugs (Chicoine et al, 2020). However, many methods used for the quantification of anthelmintic drug residues in animal‐derived foods, such as high‐performance liquid chromatography with ultraviolet (HPLC‐UV) spectroscopy (Cirkovic et al, 2015; Gili et al, 2014), ultra‐high‐pressure liquid chromatography (UHPLC) with different types of detector depending on the nature of the analytes (da Silva et al, 2017; Del Bianchi A. Cruz et al, 2018), micellar LC (Pawar et al, 2021), capillary zone electrophoresis tandem mass spectrometry (MS/MS) (Tejada‐Casado et al, 2018), and recently LC–MS/MS, have been considered as advanced systematic techniques to quantify anthelmintic drugs in various animal‐based foodstuffs (Baralla et al, 2020; Casey et al, 2021; Mooney et al, 2019; Yoo et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Optimization and validation of high‐performance liquid chromatography using modified QuEChERS to determine anthelmintic drugs in mutton

Hiwa,

Khulod

2023

Biomedical Chromatography

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The aim of this study was to rapidly determine the presence of anthelmintic drugs in sheep meat using the optimized high‐performance liquid chromatography‐ultraviolet (HPLC‐UV) method with modified QuEChERS (quick, easy, cheap, effective, rugged, safe) technology. Fifty fresh sheep meat samples from different slaughterhouses were collected. A double extraction procedure (QuEChERS/HPLC‐UV technology) was used to extract the target analytes. A multilevel calibration curve from 1 to 1000 g/kg was used to establish instrument linearity for rafoxanide, albendazole, and closantel, whereas 0.1–100 μg/kg was used for ivermectin, levamisole, and oxyclozanide to find the lowest concentration, maximum residue limit (MRL), and occupied range for targeted analytes. The concentration levels were used to investigate the linearity, whereas several certified reference materials were applied to determine accuracy. The process was linear for all combinations, from the limit of quantification (LOQ) to the maximum concentration. The LOQ was established at 0.5 μg/kg for ivermectin, levamisole, and oxyclozanide and 10 μg/kg for rafoxanide, albendazole, and closantel. Recovery values were 70%–120%, and repeatability/reproducibility stated in relative standard deviation was obtained at less than 20%. QuEChERS method revealed that most meat samples contained anthelmintic drug residues, of which the majority exceeded the MRLs. Thus, the drugs should be used correctly in animals to avoid residues in food for human consumption.

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“…Although short‐term safety and efficacy studies have been performed, little is known about optimal dosing or the chronic administration of MOX in humans (Awadzi et al, ; Cotreau et al, ). Moreover, unintentional exposure to MOX is becoming more common as it is being utilized in prophylactic regimens in the food production industry (Del Bianchi et al, ). Much attention has been placed on developing analytical methods for determining the tissue distribution of MOX in various farm animals and to quantitate drug concentrations in meat and dairy products intended for human consumption (Ali, Sun, McLeroy, & Phillippo, ; Berendsen, Mulder, & van Rhijn, ; Giannetti et al, ; Imperiale, Lifschitz, Sallovitz, Virkel, & Lanusse, ; Rübensam, Barreto, Hoff, & Pizzolato, ; Schenck & Lagman, ).…”

Section: Introductionmentioning

confidence: 99%

Bioanalytical method development and validation of moxidectin in plasma by LC–MS/MS: Application to in vitro metabolism

Chhonker

Murry

2018

Biomedical Chromatography

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Moxidectin (MOX) has recently been approved by the US Food and Drug Administration for the treatment of river blindness in select populations. It is also being evaluated as an alternative for the use of ivermectin, widespread resistance to which is becoming a global health issue. Moreover, MOX is becoming increasingly used as a prophylactic antiparasitic in the cattle industry. In this study, we developed and validated an LC–MS/MS method of MOX in human, monkey and mouse plasma. The separation was achieved on an ACE C18 (50 × 3.0 mm, 3 μm) column with isocratic elution using 0.1% acetic acid and methanol–acetonitrile (1:1, v/v) as mobile phase. MOX was quantitated using MS/MS with an electrospray ionization source operating in negative multiple reaction monitoring mode. The multiple reaction monitoring precursor ion → product ion transitions for MOX and abamectin (IS) were m/z 638.40 → 236.30 and m/z 871.50 → 565.35 respectively. The MS/MS response was linear over the concentration range 0.1–1000 ng/mL in plasma with a correlation coefficient (r2) of 0.997 or better. The within‐ and between‐day precision (relative standard deviation, RSD) and accuracy were within the acceptable limits per US Food and Drug Administration guidelines. The method was successfully applied to an in vitro metabolic stability study of MOX.

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Moxidectin residues in lamb tissues: Development and validation of analytical method by UHPLC-MS/MS

Cited by 7 publications

References 25 publications

A Review on Different Analytical Techniques for Quantification of Moxidectin

A Review on Different Analytical Techniques for Quantification of Moxidectin

Optimization and validation of high‐performance liquid chromatography using modified QuEChERS to determine anthelmintic drugs in mutton

Bioanalytical method development and validation of moxidectin in plasma by LC–MS/MS: Application to in vitro metabolism

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