Moxibustion treatment modulates the gut microbiota and immune function in a dextran sulphate sodium-induced colitis rat model

Qi, Qin; Liu, Ya Nan; Jin, Xiaoming; Zhang, Lin Shuang; Wang, Cun; Bao, Chun; Liu, Hui Rong; Wu, Huan; Wang, Xiao Mei

doi:10.3748/wjg.v24.i28.3130

Cited by 57 publications

(50 citation statements)

References 51 publications

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“…Clinical and experimental studies have shown beneficial role of acupuncture and moxibustion in reducing IBD disease activity and inflammation [20][21][22]. Our previous studies also found that both EA and moxibustion have therapeutic effect on colitis rats [4,11,23].…”

Section: Introductionmentioning

confidence: 60%

Electroacupuncture and Moxibustion Regulate Hippocampus Glia and Mitochondria Activation in DSS‐Induced Colitis Mice

Zhang

Xie

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Objectives. To study the influence of electroacupuncture (EA) and moxibustion on the hippocampus astrocyte and microglia activation in the ulcerative colitis model and to evaluate the mitochondria activity. Methods. 2.5% dextran sodium sulfate-induced colitis mice were treated by EA or moxibustion. Intestinal pathological structure was observed by hematoxylin and eosin (H&E) staining; the expression of GFAP or S100b (markers for astrocyte), Iba-1 (a marker for microglia), and Mitofilin (a marker for mitochondria) in hippocampus was detected by immunofluorescence staining or western blot. Results. The results demonstrated that both EA and moxibustion could improve the morphology of distal colonic mucosal epithelia in DSS-induced colitis mice. Expression of GFAP in the hippocampus was significantly increased after EA or moxibustion treatment. The effects were further supported by WB results. Meanwhile, expression of mitofilin in the hippocampus CA1 and CA3 regions showed the same trend as that of GFAP. Expression of Iba-1 in the hippocampus showed no significant difference after EA or moxibustion treatment, while the state of microglia changed from resting in control mice to activated state in colitis mice. Conclusion. EA and moxibustion were able to modulate the activation of astrocyte, microglial, and mitochondria in the hippocampus area in the colitis model.

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Section: Introductionmentioning

confidence: 60%

Electroacupuncture and Moxibustion Regulate Hippocampus Glia and Mitochondria Activation in DSS‐Induced Colitis Mice

Zhang

Xie

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…In addition, the phyloseq package [ 30 ] was used to plot data and perform statistical analysis in R. Metagenome function was predicted by Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) software [ 31 ]. Metabolic pathways were aligned and translated from the gene catalog against the proteins (or domains) in the KEGG databases ( ).…”

Section: Methodsmentioning

confidence: 99%

Compositional and Functional Comparisons of the Microbiota in the Colostrum and Mature Milk of Dairy Goats

Niyazbekova

Yao

Liu

et al. 2020

Animals

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Goat milk is essential for the initial development of kids by providing a great source of commensal bacteria. In this study, we analyzed the microbiota of the milk of 30 healthy Saanen dairy goats. The 30 samples comprised 15 colostrum and 15 mature milk samples, collected from three different farms of Shaanxi Province. Colostrum samples were collected daily for five days post-delivery and mature milk was collected on the 7th, 10th, 20th, 30th, and 40th days. The result showed that microbial alpha diversity was higher in the mature milk compared with that in the colostrum. Linear discriminant analysis effect size (LEfSe) was performed to detect differentially abundant taxa in colostrum and goat milk. According to taxonomy results, Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes were the predominant bacteria phyla in both colostrum and mature milk. In addition, lactation stage noticeably influenced the composition of milk microbiota. Specifically, Novosphingobium, Brachybacterium, Psychrobacter, Lactobacillus, Yersinia, Roseateles, Rothia, Sanguibacter, Cloacibacterium, Variovorax, Sphingobacterium, and Coxiella were enriched in the colostrum, while Georgenia, Peptostreptococcus, Bacteroidales, Yaniella, Planomicrobium, Cloacibacterium, Azospirillum, Turicibacter, Cupriavidus, Herbaspirillum, Rhodobacteraceae, and Aeromonadales were the dominant genera in the mature milk. The enriched metabolic functions of the goat milk microbiota were predicted by PICRUSt and classified by KEGG pathway. Moreover, the abundances of environmental information processing, cellular processes pathway, genetic information processing pathway, organismal systems pathway, and metabolism pathway were significantly different between microbiota of colostrum and mature milk. Altogether, our study disclosed the significant difference between the microbial communities of colostrum and mature milk and provided grounds for further research in dairy microbiology.

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“…Experimental and clinical studies have shown that acupuncture is a safe and effective treatment, and a meta-analysis has shown that acupuncture is more effective than oral sulphasalazine in the treatment of IBD[ 65 ]. Moxibustion plays a therapeutic role by repairing mucosal tissue damage, regulating gut microbiota and improving intestinal mucosal immunity[ 66 , 67 ] and short-term (7 d) rather than long-term (14 d) moxibustion may significantly affect the gut microbiota[ 66 ]. Studies by Wei et al[ 68 ] have shown that both electroacupuncture and moxibustion can improve anxiety behaviors in patients with colitis, and its effect comes in part from improving the balance of the HPA axis.…”

Section: Microbiota-gut-liver-brain Axis and Ibdmentioning

confidence: 99%

Role of gut microbiota via the gut-liver-brain axis in digestive diseases

Ding¹,

Jin²,

Yang³

et al. 2020

WJG

104

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The gut-brain axis is a bidirectional information interaction system between the central nervous system (CNS) and the gastrointestinal tract, in which gut microbiota plays a key role. The gut microbiota forms a complex network with the enteric nervous system, the autonomic nervous system, and the neuroendocrine and neuroimmunity of the CNS, which is called the microbiota-gut-brain axis. Due to the close anatomical and functional interaction of the gut-liver axis, the microbiota-gut-liver-brain axis has attracted increased attention in recent years. The microbiota-gut-liver-brain axis mediates the occurrence and development of many diseases, and it offers a direction for the research of disease treatment. In this review, we mainly discuss the role of the gut microbiota in the irritable bowel syndrome, inflammatory bowel disease, functional dyspepsia, non-alcoholic fatty liver disease, alcoholic liver disease, cirrhosis and hepatic encephalopathy via the gut-liver-brain axis, and the focus is to clarify the potential mechanisms and treatment of digestive diseases based on the further understanding of the microbiota-gut- liver-brain axis.

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Moxibustion treatment modulates the gut microbiota and immune function in a dextran sulphate sodium-induced colitis rat model

Abstract: Our findings suggest that moxibustion exerts its therapeutic effect by repairing mucosal tissue damage and modulating the gut microbiome and intestinal mucosal immunity.

Cited by 57 publications

References 51 publications

Electroacupuncture and Moxibustion Regulate Hippocampus Glia and Mitochondria Activation in DSS‐Induced Colitis Mice

Electroacupuncture and Moxibustion Regulate Hippocampus Glia and Mitochondria Activation in DSS‐Induced Colitis Mice

Compositional and Functional Comparisons of the Microbiota in the Colostrum and Mature Milk of Dairy Goats

Role of gut microbiota via the gut-liver-brain axis in digestive diseases

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