Moving tissue spectral window to the deep‐ultraviolet via optical clearing

Carneiro, Isa; Carvalho, Sónia; Henrique, Rui; Oliveira, Luís; Tuchin, Valery V.

doi:10.1002/jbio.201900181

Cited by 20 publications

(28 citation statements)

References 34 publications

(45 reference statements)

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“…Current optical diagnostic and therapeutic methods work at wavelengths within the traditional tissue windows: I (625-975 nm), II (1100-1350 nm), III (1600-1870 nm), and IV (2100-2300 nm) [3,4]. Complementary to these wavelength ranges, where light penetration depth presents local maxima for natural tissues [2,5], the use of optical clearing treatments can induce other optical diagnostic and treatment windows, as it was recently demonstrated for the ultraviolet (UV) range with transmittance efficiency peaks at (230 ± 30), (275 ± 25 nm), and (300 ± 40 nm) [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…Steven Jacques in his widely cited review paper (more than 2500 citations in Google Scholar) has summarized the wavelength dependencies of the optical properties of different biological tissues [20]. According to this paper, the absorption coefficient shows a decreasing behavior with increasing wavelength, since major chromophores, such as proteins, DNA and hemoglobin present their absorption bands in the UV and visible range [6,20]. In addition, due to the presence of water or lipids, some other absorption bands might occur in the NIR range.…”

Section: Introductionmentioning

confidence: 99%

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Lipofuscin-Type Pigment as a Marker of Colorectal Cancer

et al. 2020

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The study of the optical properties of biological tissues for a wide spectral range is necessary for the development and planning of noninvasive optical methods to be used in clinical practice. In this study, we propose a new method to calculate almost all optical properties of tissues as a function of wavelength directly from spectral measurements. Using this method, and with the exception of the reduced scattering coefficient, which was obtained by traditional simulation methods, all the other optical properties were calculated in a simple and fast manner for human and pathological colorectal tissues. The obtained results are in good agreement with previous published data, both in magnitude and in wavelength dependence. Since this method is based on spectral measurements and not on discrete-wavelength experimental data, the calculated optical properties contain spectral signatures that correspond to major tissue chromophores such as DNA and hemoglobin. Analysis of the absorption bands of hemoglobin in the wavelength dependence of the absorption spectra of normal and pathological colorectal mucosa allowed to identify differentiated accumulation of a pigment in these tissues. The increased content of this pigment in the pathological mucosa may be used for the future development of noninvasive diagnostic methods for colorectal cancer detection.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Lipofuscin-Type Pigment as a Marker of Colorectal Cancer

et al. 2020

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“…Current optical diagnostic and therapeutic methods work at wavelengths within the traditional tissue windows: I (625-975 nm), II (1100-1350 nm), III (1600-1870 nm) and IV (2100-2300 nm) [3,4]. Complementary to these wavelength ranges, where light penetration depth presents local maxima for natural tissues [2,5], the use of optical clearing treatments can induce other optical diagnostic and treatment windows, as it was recently demonstrated for the ultraviolet (UV) range with transmittance efficiency peaks at (23030), (27525 nm) and (30040 nm) [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…Steven Jacques in his widely cited review paper (more than 2500 citations in Google Scholar) has summarized the wavelength dependencies of the optical properties of different biological tissues [20]. According to this paper, the absorption coefficient shows decreasing behavior with increasing wavelength, since major chromophores, such as proteins, DNA and hemoglobin present their absorption bands in the UV and visible range [6,20]. In addition, due to the presence of water or lipids, some other absorption bands might occur in the NIR range.…”

Section: Introductionmentioning

confidence: 99%

Lipofuscin-Type Pigment as a Marker of Colorectal Cancer

Carvalho¹,

Carneiro²,

Henrique³

et al. 2020

Preprint

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The study of the optical properties of biological tissues for a wide spectral range is necessary for the development and planning of noninvasive optical methods to be used in clinical practice. In this study, we propose a new method to calculate almost all optical properties of tissues as a function of wavelength directly from spectral measurements. Using this method and with the exception of the reduced scattering coefficient, which was obtained by traditional simulation methods, all the other optical properties were calculated in a simple and fast manner for human and pathological colorectal tissues. The obtained results are in good agreement with previous published data, both in magnitude and in wavelength dependence. Since this method is based on spectral measurements and not on discrete-wavelength experimental data, the calculated optical properties contain spectral signatures that correspond to major tissue chromophores such as DNA and hemoglobin. Analysis of the absorption bands of hemoglobin in the wavelength dependence of the absorption spectra of normal and pathological colorectal mucosa allowed to identify differentiated accumulation of a pigment in these tissues. The increased content of this pigment in the pathological mucosa may be used for the future development of noninvasive diagnostic methods for colorectal cancer detection.

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“…As the sample loses water, its scatterers approach each‐other, arranging themselves in a better ordered fashion (packing), leading to a decreased sample thickness. A brief increase in μ s may be created by this tighter packing , but due to a decreased sample thickness, tissue transparency increases . At the same time, the OCA molecules diffuse from the solution into the interstitial locations of the tissue, forcing scatterers to separate once again (tissue swelling).…”

Section: Introductionmentioning

confidence: 99%

A robust ex vivo method to evaluate the diffusion properties of agents in biological tissues

Carneiro

Carvalho

Henrique

et al. 2019

Journal of Biophotonics

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A robust method is presented for evaluating the diffusion properties of chemicals in ex vivo biological tissues. Using this method that relies only on thickness and collimated transmittance measurements, the diffusion properties of glycerol, fructose, polypropylene glycol and water in muscle tissues were evaluated. Amongst other results, the diffusion coefficient of glycerol in colorectal muscle was estimated with a value of 3.3 × 10−7 cm2/s. Due to the robustness and simplicity of the method, it can be used in other fields of biomedical engineering, namely in organ cryoprotection and food industry.

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Moving tissue spectral window to the deep‐ultraviolet via optical clearing

Cited by 20 publications

References 34 publications

Lipofuscin-Type Pigment as a Marker of Colorectal Cancer

Lipofuscin-Type Pigment as a Marker of Colorectal Cancer

Lipofuscin-Type Pigment as a Marker of Colorectal Cancer

A robust ex vivo method to evaluate the diffusion properties of agents in biological tissues

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