Moving pieces in a taxonomic puzzle: Venom 2D-LC/MS and data clustering analyses to infer phylogenetic relationships in some scorpions from the Buthidae family (Scorpiones)

Nascimento, Danielle G.; Rates, Breno; Santos, Daniel Moreira dos; Verano‐Braga, Thiago; Barbosa-Silva, Adriano; Dutra, A.A.A.; Biondi, Ilka; Lima, Maria Elena de; Pimenta, Adriano M.C.

doi:10.1016/j.toxicon.2006.01.015

Cited by 87 publications

(48 citation statements)

References 30 publications

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“…Although a few early studies (e.g., Bucherl 1953) considered toxic effects of the venom of Tityus bahiensis (herein TbScV), for years it remained little studied when compared to Tityus serrulatus, the most widely studied venom of the genus (Gomez and Diniz 1966;Lima et al 1986;Becerril et al 1996). Recently there has been renewed interest in TbScV, and several studies have examined immunological cross-reactivity (Nishikawa et al 1994;Alvarenga et al 2002;Borges et al 2008), peptide toxin sequences (Pimenta et al 2001), and proteonomic relationships (Nascimento et al 2006) among the Tityus venoms. Despite these advances, little is known about sodium channel modification by TbScV.…”

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confidence: 99%

Differential Effects of Tityus bahiensis Scorpion Venom on Tetrodotoxin-Sensitive and Tetrodotoxin-Resistant Sodium Currents

et al. 2009

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We examined modification of sodium channel gating by Tityus bahiensis scorpion venom (TbScV), and compared effects on native tetrodotoxin-sensitive and tetrodotoxin-resistant sodium currents from rat dorsal root ganglion neurons and cardiac myocytes. In neurons, TbScV dramatically reduced the rate of sodium current inactivation, increased current amplitude, and caused a negative shift in the voltage-dependence of activation and inactivation of tetrodotoxin-sensitive channels. Enhanced activation of modified sodium channels was independent of a depolarizing prepulse. We identified two components of neuronal tetrodotoxin-resistant current with biophysical properties similar to those described for NaV1.8 and NaV1.9. In contrast to its effects on neuronal tetrodotoxin-sensitive current, TbScV caused a small decrease in neuronal tetrodotoxin-resistant sodium current amplitude and the gating modifications described above were absent. A third tetrodotoxin-resistant current, NaV1.5 recorded in rat cardiac ventricular myocytes, was inhibited approximately 50% by TbScV, and the remaining current exhibited markedly slowed activation and inactivation. In conclusion, TbScV has very different effects on different sodium channel isoforms. Among the neuronal types, currents resistant to tetrodotoxin are also resistant to gating modification by TbScV. The cardiac tetrodotoxin-resistant current has complex sensitivity that includes both inhibition of current amplitude and slowing of activation and inactivation.

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confidence: 99%

Differential Effects of Tityus bahiensis Scorpion Venom on Tetrodotoxin-Sensitive and Tetrodotoxin-Resistant Sodium Currents

et al. 2009

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“…All of them were treated with scorpion antivenom but died within 4 h, 8 h, and 30 h after of the accidents. T. stigmurus venom is the most toxic (DL 50 ¼ 0.773 mg/kg) when compared to T. serrulatus and T. bahiensis (Nishikawa et al 1994;Venancio et al 2013), although the proteomic profile places T. stigmurus and T. serrulatus as very close species and T. bahiensis the less related species in the Tityus genus (Nascimento et al 2006). One hundred distinct components have been described in T. stigmurus venom, showing molecular masses from 216.5 to 44,800 Da (Batista et al 2007).…”

Section: Scorpions and Scorpionism In The Northeast Of Brazilmentioning

confidence: 99%

Scorpionism and dangerous species of Brazil

et al. 2014

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Brazil is a country with a large territorial extension (approximately 8.5 million kilometers squared), which is composed of 26 states and a Federal District. The scorpion fauna of Brazil is also quite vast, highlighting the Tityus genus. The species that is considered the most dangerous and that is accounted for the highest number of accidents is Tityus serrulatus. One of the unique features of this species is its ability to reproduce by parthenogenesis, which enabled its rapid proliferation across the country. According to the records of the Ministry of Health, during the years (2000)(2001)(2002)(2003)(2004)(2005)(2006)(2007)(2008)(2009)(2010)(2011)(2012), accidents related to scorpions have increased dramatically all over the country and are recognized as a public health problem by the government. In this period, 482,479 cases of scorpion envenomation have been reported in Brazil, with the occurrence of 749 deaths for a lethal rate of 0.15 %.

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“…Several recent studies on scorpion, spider, snake and cone snail venoms have demonstrated that venom peptides number in the hundreds [12], with up to 1000 masses detected in spider venom by MALDI-TOF MS [5] or in Conus venom by electrospray-qTOF MS [13,14]. A conservative estimate of the total number of peptides in all described venomous animal species approaches 20 million and will certainly rise in the future with increases in the performance of MS instrumentation.…”

Section: Proteomics-based Investigation Strategies: Venom Profiling Fmentioning

confidence: 99%

Venomics as a drug discovery platform

Escoubas

King

2009

Expert Review of Proteomics

163

117

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Moving pieces in a taxonomic puzzle: Venom 2D-LC/MS and data clustering analyses to infer phylogenetic relationships in some scorpions from the Buthidae family (Scorpiones)

Cited by 87 publications

References 30 publications

Differential Effects of Tityus bahiensis Scorpion Venom on Tetrodotoxin-Sensitive and Tetrodotoxin-Resistant Sodium Currents

Differential Effects of Tityus bahiensis Scorpion Venom on Tetrodotoxin-Sensitive and Tetrodotoxin-Resistant Sodium Currents

Scorpionism and dangerous species of Brazil

Venomics as a drug discovery platform

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