Moving perfusion culture and live-cell imaging from lab to disc: proof of concept toxicity assay with AI-based image analysis

Abstract

In vitro, cell-based assays are essential in diagnostics and drug development. There are ongoing efforts to establish new technologies that enable real-time detection of cell-drug interaction during culture under flow...

APELLA: Open-Source, miniaturized All-in-One powered Lab-on-a-Disc platform

“…Moreover, the disposable disc can be mass-produced, reducing the risk of sample cross-contamination. Thus, LoDs have been used for point-of-care testing [6] , [9] , biochemical assays [10] , environmental analysis [4] , [10] , and cell-based assays [11] , [12] .…”

“…Recent studies have shown how wireless power transmission can be used to power active electrical components on a rotating disc [26] , [27] , [28] , which opens up a myriad of possibilities for on-disc applications such as actuation [29] , [30] , electrochemical [19] , [31] , chemiluminescence [8] , microscopic imaging [32] , [33] , and cell culture monitoring [34] . The development of wireless-powered LoD systems shows the potential of this platform to be used for on-site detection, considering the substantial reduction of auxiliary instrumentation [8] .…”

Traditional Bulletin Board: Benefits and Drawbacks

“…The assurance against outside interference and the ease of data transfer are aspects of the data transmission method that are related to the security of this technology [24,26,27]. Resistance, affordability, energy efficiency, simplicity of usage, and minimal complexity are the main and advantageous aspects [28]. Microchips operating at 2.4 GHz are required for usable devices, as well as the ability to receive and transmit data across numerous bandwidth regions [29][30][31].…”