Moving into a new dimension: Tracking migrating cells with digital holographic cytometry in 3D

Wingren, Anette Gjörloff

doi:10.1002/cyto.a.23679

Cited by 6 publications

(6 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A more cell-friendly and speedy method of investigating morphological differences would facilitate CTC research. Digital holographic cytometry (DHC) is a powerful tool for label-free cell observations and the evaluation of cell morphological and dynamical parameters in vitro [20][21][22][23][24][25][26]. Studies using DHC include different cell types, from protozoa, bacteria and plant cells to mammalian cells such as nerve cells, stem cells and tumor cells [23].…”

Section: Introductionmentioning

confidence: 99%

Discrimination between Breast Cancer Cells and White Blood Cells by Non-Invasive Measurements: Implications for a Novel In Vitro-Based Circulating Tumor Cell Model Using Digital Holographic Cytometry

El-Schich

Janicke²,

Alm³

et al. 2020

Applied Sciences

View full text Add to dashboard Cite

Breast cancer is the second most common cancer worldwide. Metastasis is the main reason for death in breast cancer, and today, there is a lack of methods to detect and isolate circulating tumor cells (CTCs), mainly due to their heterogeneity and rarity. There are some systems that are designed to detect rare epithelial cancer cells in whole blood based on the most common marker used today, the epithelial cell adhesion molecule (EpCAM). It has been shown that aggressive breast cancer metastases are of non-epithelial origin and are therefore not always detected using EpCAM as a marker. In the present study, we used an in vitro-based circulating tumor cell model comprising a collection of six breast cancer cell lines and white blood cell lines. We used digital holographic cytometry (DHC) to characterize and distinguish between the different cell types by area, volume and thickness. Here, we present significant differences in cell size-related parameters observed when comparing white blood cells and breast cancer cells by using DHC. In conclusion, DHC can be a powerful diagnostic tool for the characterization of CTCs in the blood.

show abstract

Section: Introductionmentioning

confidence: 99%

Discrimination between Breast Cancer Cells and White Blood Cells by Non-Invasive Measurements: Implications for a Novel In Vitro-Based Circulating Tumor Cell Model Using Digital Holographic Cytometry

El-Schich

Janicke²,

Alm³

et al. 2020

Applied Sciences

View full text Add to dashboard Cite

show abstract

“…For the last decade, there have been significant developments in the instrumentations of QPI. [2,7,[11][12][13][14][15][16][17] For example, the spatial and temporal resolution of QPI had been improved and reached the limitations of farfield diffraction resolution and the speed of a high-speed image sensor. [18][19][20][21][22] Recently, many researchers have intensively reported biological applications of QPI, in particular, medical applications.…”

Section: Methods Detailsmentioning

confidence: 99%

Label-free imaging and analysis of subcellular parts of a living diatom cylindrotheca sp. using optical diffraction tomography

et al. 2020

View full text Add to dashboard Cite

Optical diffraction tomography is an emerging label-free microscopic technique with its capability of label-free, quantitative, and rapid imaging of biological samples. In this work, we present the imaging and analysis of a living diatom Cylindrotheca sp. in seawater without using any pretreatment such as fluorescence staining. The 3D refractive index (RI) of a living diatom cell was measured, to which quantitative image analysis was perform to investigate subcellular parts of the diatom. Each part of the cell was well distinguished as RI values and distributions. From the analysis, RI values of frustules, protoplasm, vacuole, and chloroplast were estimated to be in the range of 1.

show abstract

“…Cell motility is an important feature as it is suggestive of invasiveness and, when monitored along organoid formation, arguably informs about the metastatic capacity of tumor cells [17]. Nonetheless, quantitative methods for studying these early events are scarce, as conventional migration assays such as wound-healing and transwell assays do not evaluate growth in 3D [18]. In turn, in vitro 3D cultures are commonly imaged using bright-field, fluorescence, or phase-contrast microscopy [19], where organoid formation is not continuously monitored.…”

Section: Discussionmentioning

confidence: 99%

Subpopulations of Organoid-Forming Cells Have Different Motility

et al. 2020

View full text Add to dashboard Cite

Cancer stem cells from oropharyngeal squamous cell carcinoma (OPSCC) have the ability to self-renew and differentiate into heterogeneous three-dimensional structures carrying features of tumor cells. Here, we describe a simple and label-free method for generating tumor organoids, and imaging them using live digital holographic microscopy (DHM) on the basis of the phase shift caused by light passing through the cells. We show early events of cell aggregation during tumor-organoid formation, and display their heterogeneity in terms of optical parameters up to an optical volume of 105 µm3. Lastly, by sorting OPSCC epithelial cells, we demonstrate that CD44+ cells displayed greater motility and tumor-forming capacity than those of CD44− cells. These results were in line with previous reports highlighting increased invasive and tumorigenic potential in tumor cells expressing high levels of CD44. Our method provides insight into the formation of tumor organoids, and could be used to assess stemness-associated biomarkers and drug screenings on the basis of tumor organoids.

show abstract

Moving into a new dimension: Tracking migrating cells with digital holographic cytometry in 3D

Cited by 6 publications

References 15 publications

Discrimination between Breast Cancer Cells and White Blood Cells by Non-Invasive Measurements: Implications for a Novel In Vitro-Based Circulating Tumor Cell Model Using Digital Holographic Cytometry

Discrimination between Breast Cancer Cells and White Blood Cells by Non-Invasive Measurements: Implications for a Novel In Vitro-Based Circulating Tumor Cell Model Using Digital Holographic Cytometry

Label-free imaging and analysis of subcellular parts of a living diatom cylindrotheca sp. using optical diffraction tomography

Subpopulations of Organoid-Forming Cells Have Different Motility

Contact Info

Product

Resources

About