Moving from millifluidic to truly microfluidic sub-100-μm cross-section 3D printed devices

Beauchamp, Michael J.; Nordin, Gregory P.; Woolley, Adam T.

doi:10.1007/s00216-017-0398-3

Cited by 107 publications

(86 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Second, we used a 4% PEGDA solution: we found that 3% PEGDA was not sufficient to eliminate the undesired migration of analyte while 5% PEGDA tended to block the channels and prevent fluid flow. While these COC devices are compatible with standard 2D micromachining, we note that it is also feasible to 3D print microfluidic devices from PEGDA . These 3D printed devices may be appealing for future analyses to avoid this photografting step.…”

Section: Resultsmentioning

confidence: 99%

Microchip electrophoresis separation of a panel of preterm birth biomarkers

Nielsen

Sonker

et al. 2018

Electrophoresis

Self Cite

View full text Add to dashboard Cite

Preterm birth (PTB) is responsible for over one million infant deaths annually worldwide. Often, the first and only indication of PTB risk is the onset of early labor. Thus, there is an urgent need for an early PTB risk diagnostic that is inexpensive, reliable, and robust. Here, we describe the development of a microchip electrophoresis (μCE) method for separating a mixture of six PTB protein and peptide biomarkers present in maternal blood serum. μCE devices were photografted with a poly(ethylene glycol) diacrylate surface coating to regulate EOF and reduce nonspecific analyte adsorption. Separation conditions including buffer pH, buffer concentration, and applied electric field were varied to improve biomarker peak resolution while minimizing deleterious effects like Joule heating. In this way, it was possible to separate six PTB biomarkers, the first μCE separation of this biomarker panel. LODs were also measured for each of the six PTB biomarkers. In the future, this μCE separation can be integrated with upstream maternal blood serum sample preparation steps to yield a complete PTB risk diagnosis microdevice.

show abstract

Section: Resultsmentioning

confidence: 99%

Microchip electrophoresis separation of a panel of preterm birth biomarkers

Nielsen

Sonker

et al. 2018

Electrophoresis

Self Cite

View full text Add to dashboard Cite

show abstract

“…While this approach is suitable to fabricate microchannels with different cross-sections, the resolution of printed parts is not high enough due to inherent limitations of FDM printing. Although direct fabrication of microchannels using SLA and digital light processing (DLP) method is a suitable candidate, inertial microfluidic devices often operate in channels in the order of micrometer (e.g., rectangular with 200 µm width and 40 µm height) where removing resin residuals from the channel is a challenging issue 41 .…”

mentioning

confidence: 99%

3D Printing of Inertial Microfluidic Devices

Bazaz

Rouhi

Raoufi

et al. 2020

Sci Rep

130

View full text Add to dashboard Cite

Inertial microfluidics has been broadly investigated, resulting in the development of various applications, mainly for particle or cell separation. Lateral migrations of these particles within a microchannel strictly depend on the channel design and its cross-section. Nonetheless, the fabrication of these microchannels is a continuous challenging issue for the microfluidic community, where the most studied channel cross-sections are limited to only rectangular and more recently trapezoidal microchannels. As a result, a huge amount of potential remains intact for other geometries with cross-sections difficult to fabricate with standard microfabrication techniques. In this study, by leveraging on benefits of additive manufacturing, we have proposed a new method for the fabrication of inertial microfluidic devices. In our proposed workflow, parts are first printed via a high-resolution DLP/SLA 3D printer and then bonded to a transparent PMMA sheet using a double-coated pressure-sensitive adhesive tape. Using this method, we have fabricated and tested a plethora of existing inertial microfluidic devices, whether in a single or multiplexed manner, such as straight, spiral, serpentine, curvilinear, and contraction-expansion arrays. Our characterizations using both particles and cells revealed that the produced chips could withstand a pressure up to 150 psi with minimum interference of the tape to the total functionality of the device and viability of cells. As a showcase of the versatility of our method, we have proposed a new spiral microchannel with right-angled triangular cross-section which is technically impossible to fabricate using the standard lithography. We are of the opinion that the method proposed in this study will open the door for more complex geometries with the bespoke passive internal flow. Furthermore, the proposed fabrication workflow can be adopted at the production level, enabling large-scale manufacturing of inertial microfluidic devices.

show abstract

“…Although the channel walls and edges are less smooth compared to PDMS chips, this is often negligible, as flow-based microfluidics for cultivation of tissues or whole organisms often do not require high-resolution chips with mm details. Furthermore, even complex systems including valves have been achieved using this method of production (Beauchamp et al, 2017;Bhattacharjee et al, 2016;Gong et al, 2016), and it is foreseeable that such approaches will ultimately be able to compete with soft lithography. A clear advantage is the minimal equipment required (plastic printers are available starting from a few hundred US dollars) and the high level of automation (designs from other groups can simply be imported and manufactured without any additional manual work).…”

Section: Discussionmentioning

confidence: 99%

Microfluidics as an Emerging Precision Tool in Developmental Biology

Sonnen

Merten

2019

Developmental Cell

View full text Add to dashboard Cite

Microfluidics has become a precision tool in modern biology. It enables omics data to be obtained from individual cells, as compared to averaged signals from cell populations, and it allows manipulation of biological specimens in entirely new ways. Cells and organisms can be perturbed at extraordinary spatiotemporal resolution, revealing mechanistic insights that would otherwise remain hidden. In this perspective article, we discuss the current and future impact of microfluidic technology in the field of developmental biology. In addition, we provide detailed information on how to start using this technology even without prior experience.

show abstract

Moving from millifluidic to truly microfluidic sub-100-μm cross-section 3D printed devices

Cited by 107 publications

References 51 publications

Microchip electrophoresis separation of a panel of preterm birth biomarkers

Microchip electrophoresis separation of a panel of preterm birth biomarkers

3D Printing of Inertial Microfluidic Devices

Microfluidics as an Emerging Precision Tool in Developmental Biology

Contact Info

Product

Resources

About