Movements of Calcium During Tension Development Induced by Barium and High-Potassium in Guinea Pig Taenia Coli

Karaki, Hideaki; Ikeda, Miyoshi; Urakawa, Norimoto

doi:10.1254/jjp.19.291

Cited by 36 publications

(21 citation statements)

References 13 publications

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“…The factors inhibiting the high-K induced tension, viz., ouabain, Li substitution, DNP, glucose removal and anoxia, decreased the increased size of TBF by high-K to the level in normal medium. These results confirm the suggestion that Ca fraction which did not exchange within 4 minutes (TBF) may be closely related to tension development in taenia coli (5,6,10,11). The increased tissue Ca in high-K medium was reduced by DNP, glucose removal and anoxia to the control level although ouabain and Li substitution did not significantly alter it (P<0.01).…”

Section: Discussionsupporting

confidence: 85%

EFFECTS OF OUABAIN AND METABOLIC INHIBITING FACTORS ON Ca DISTRIBUTION DURING K-INDUCED CONTRACTURE IN GUINEA PIG TAENIA COLI

Urakawa

Karaki

Ikeda

1970

Japanese Journal of Pharmacology

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It was reported that an application of high-K (isotonic, 40 mm) caused an increase in oxygen consumption accompanied by an increase in tension change in guinea pig taenia coli (1) and that these changes were reversibly abolished by a depletion of Ca from the high-K medium and Sr could replace Ca only for oxygen consumption (2). These results suggest that Ca entering smooth muscle treated with high-K probably increases oxygen consumption, independent of an increase in muscle tension. In a previous paper (3), effects of factors inhibiting tension development on oxygen consumption of the muscle in high-K medium were reported. Glucose removal from the high-K medium abolished the already developed tension corresponded with a decrease in the elevated oxygen con sumption rate. Addition of DNP (1 x 10-'m) to high-K medium further increased or maintained the increased oxygen consumption although it abolished the already develop ed tension, and the change in oxygen consumption was not modified by Ca removal. On the other hand ouabain (2.5 x 10-8 M) maintained the increased oxygen consumption in high-K medium, but almost abolished the developed tension. The increase in oxygen consumption was dependent on external calcium.In the present paper, effects of factors inhibiting high-K induced tension and/or oxygen consumption on Ca distribution in taenia coli are studied and this throws some light on a participation of Ca distribution in changes in tension and oxygen consumption of the muscle in high-K medium.A study on ouabain and DNP with high-K on Ca exchange in taenia coli has been reported by Pfaffman and Holland (4), which is incompatible with the present data. METHODSStrips of taenia coli isolated from white male guinea pigs were suspended in an organ bath containing Tyrode solution of the following composition (mm); NaC1, 136.8; KCI, 2.7; CaCl2j 2.5; MgCl,, 1.0; NaH,P0,, 0.4; NaHCO,j 11.9 and glucose, 5.5. The solu tion was saturated with 95% 02 and 5 % CO, mixture and maintained at 37°C. Tension changes were isometrically recorded with a mechanoelectro-transducer.

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Section: Discussionsupporting

confidence: 85%

EFFECTS OF OUABAIN AND METABOLIC INHIBITING FACTORS ON Ca DISTRIBUTION DURING K-INDUCED CONTRACTURE IN GUINEA PIG TAENIA COLI

Urakawa

Karaki

Ikeda

1970

Japanese Journal of Pharmacology

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“…2, 0.1 mM Ca increased 02 consumption without an accompanying tension development. Urakawa et al reported that during the tonic contraction of the muscle induced by 40 mM K, Call enters into the cell across the cell membrane (2,22), and that Sr++ also entered into the cell in 40 mM K medium accompanied by little tension and large 02 consumption (6,23). Although up to the present, there is little information about the affinity of Call to Mt and troponin in physiological environment, it is suggested that one part of the Call entering smooth muscle treated with high-K increases 02 consumption independent of an increase in muscle tension.…”

Section: Discussionmentioning

confidence: 99%

The Regulation of Respiration of Guinea Pig Taenia Coli in High-K Medium; The Role of Nicotinamide-Adenine Dinucleotide, Adenosine Diphosphate and Ca++

Tsuda

Urakawa

Saitō

et al. 1975

Japanese Journal of Pharmacology

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Abstract-In an attempt to elucidate the regulation mechanism of respiration in the smooth muscle cell, we investigated the roles of nicotinamide-adenine dinucleotide (NAD), adenosine diphosphate (ADP) and Ca" in the muscle respiration using the tissues and subcellular fractions from guinea pig taenia coli. The tension in the strips of taenia coli increased with a concomitant increase in 02 consumption in high-K medium (40 mM K) containing 2.5 mM Ca. 10-3 M amytal and 10-6 M ouabain decreased the high-K induced tension and 02 consumption of the muscle. 10-4 M 2,4-dinitrophenol (DNP) relieved the decreased respiration induced by ouabain, but not that with amytal. From these data it is suggested that NADH-linked respiration plays an important role in the respiration of the muscle. Ca++ in concentrations ranging from 0.5 to 2.5 mM in the high-K medium resulted in an increase in tension and in 02 consumption progressively. In spectrophotometric observations of subcellular fractions of the taenia coli, ADP increased in absorbance change at 340 mtg. Such occurred in mitochondrial fractions and was initiated by the addition of NADH. Therefore it is deduced that the increase in ADP level of the cytoplasm is primarily due to a contraction triggered by Ca" thus stimulating respiration. On the other hand, at 0.1 mM of Ca++ concentration, the muscle strip increased O2 consumption without tension development in high-K medium. In the spectrophotometric obser vations, Ca++ and Sr++ increased the absorbance change in the homogenate and in the mitochondrial fraction. Hence, it seems that one part of the Ca++ entering into the smooth muscle treated with the high-K increased O2 consumption in mitochondria independent of an increase in muscle tension. From these results it is concluded that NADH-linked respiration plays an important role in the smooth muscle respi ration in high-K medium and that ADP and Ca++ also play a role in regulating res piration.It has been reported that the smooth muscle of guinea pig taenia coli requires the aerobic breakdown of carbohydrate to maintain a continuous tension in a high-K (40 mM) medium(1) and that during the tonic contraction, Ca++ enters across the cell membrane into the muscle (2) and also that both the changes in tension and Ca++ movement induced by the high-K are abolished by an application of 10-4M DNP or anoxia (3). In preliminary work we have found that the inhibitors of NADH oxidation in mitochondrial respiratory

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“…These results might be explained by differences in the experimental procedures, or a variety of causes, including alteration in the extracellular space associated with contraction, and redistribution of the calcium taken up to different internal fractions during contraction. KARAKI et al(1969) reported that no change in Ca2+ uptake occurs in the first 5 min of K-induced contracture. The methods used in the present 45Ca-uptake experiments (5 min contracture followed by 10 sec wash and blot) produced results in the controls which were in agreement with those of Karaki et al It appears that the results of 60 min of PLC treatment seen in Fig.…”

Section: Discussionmentioning

confidence: 99%

Effects of Phospholipase C on Electrical and Mechanical Activity of Guinea Pig Stomach Muscle

Sakata

1980

Jpn.J.Physiol

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Effects of phospholipase C (PLC) on factors involved in smooth muscle contraction were investigated in longitudinal muscle of the guinea pig antrum. This agent irreversibly reduced spontaneous contraction and action potentials; partially after 30 min and, sometimes , completely after 60 min of exposure to PLC. The membrane depolarized about 8 mV from the control level in 30 min and 16 mV in 60 min. ACh-and KC1-induced contracture were inhibited in an external Ca2+ concentration range from zero to three times normal. The phasic component of KC1-induced contracture was abolished occasionally in normal Ca2+ concentration, and frequently in Ca2+ -free solution. Ca-uptake by antrum tissue, which was the same for control, ACh-, and KC1-induced contracture before PLC treatment, was not statistically different in PLCtreated control, but the value decreased during ACh-induced, and increased during KCl-induced contracture after PLC treatment. The contents of phosphatidylcholine and phosphatidylethanolamine were reduced slightly after PLC treatment, but this reduction was statistically significant. Substantial damage and modification of physical characteristics appeared in electron micrographs of PLC-treated preparations . From the results it was concluded that changes in Ca2+ transport and membrane structural damage both contribute to inhibition of smooth muscle activity by PLC.In addition to their function as the principal structural component of cell membranes (ROBERTSON, 1960;SINGER and NICOLSON, 1972), phospholipids also contribute to ion exchange (TomAs, 1964;GOLDMAN, 1965), and enzyme functions (MARToNosi et al., 1968;VESSEY and ZAKIM, 1971) in membranes.Phospholipase C (PLC) effectively inhibits the ionic transport responsible for the generation of action potentials in skeletal muscle and nerves (TomAs, 1958;ALBUQUERQUE and THESLEFF, 1967). It was suggested that the PLC effect on inhibition of the ionic transport is through its action on the hydrophilic heads of certain membrane phospholipids, but the actual mechanism is not yet clear .

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Movements of Calcium During Tension Development Induced by Barium and High-Potassium in Guinea Pig Taenia Coli

Cited by 36 publications

References 13 publications

EFFECTS OF OUABAIN AND METABOLIC INHIBITING FACTORS ON Ca DISTRIBUTION DURING K-INDUCED CONTRACTURE IN GUINEA PIG TAENIA COLI

EFFECTS OF OUABAIN AND METABOLIC INHIBITING FACTORS ON Ca DISTRIBUTION DURING K-INDUCED CONTRACTURE IN GUINEA PIG TAENIA COLI

The Regulation of Respiration of Guinea Pig Taenia Coli in High-K Medium; The Role of Nicotinamide-Adenine Dinucleotide, Adenosine Diphosphate and Ca++

Effects of Phospholipase C on Electrical and Mechanical Activity of Guinea Pig Stomach Muscle

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