Movement of water in fish otoliths

Population structure of the black seabream Spondyliosoma cantharus along the south‐west Portuguese coast inferred from otolith chemistry

“…Right sagittal otoliths were rinsed in ethanol, brushed and sonicated in Milli‐Q water to remove the glycerol (Campana, 1999; Stirratt, 2001). Most clearing agents would require a period of a few weeks or months to penetrate otoliths (Gauldie et al , 1998). In the case of glycerol, it would take 1–2 months of immersion to enter otoliths (Campana et al , 2003).…”

Biogenic and diagenetic indicators in archaeological and modern otoliths: Potential and limits of high definition synchrotron micro-XRF elemental mapping

“…2a and S4), we did not observe depletion in K at the sample periphery. It is generally expected that K is one of the more easily soluble elements in otoliths because it is presumed associated with the organic matrix rather than in the aragonite lattice (Gauldie et al, 1998;Proctor and Thresher, 1998). This is an indication of limited post-mortem alteration of these samples.…”

Insights into population structure of Diplodus vulgaris along the SW Portuguese coast from otolith elemental signatures

“…Shortly after ageing, the right otoliths were rinsed in ethanol and brushed and sonicated in Milli-Q water to remove any glycerol (Campana, 1999;Stirratt, 2001). Glycerol would require a period of a few weeks or months to permeate the microchannel architecture of the otoliths and would be unlikely to have penetrated to the otolith core region analysed by laser ablation (Gauldie et al, 1998).…”