Nitrogen (N) movement in the soil resulting from the long-term application of fertilizer N is an environmental concern when it reaches the groundwater. The distribution of N in the profile of an alkaline sandy loam soil (Typic Haplustept) and its relationship with N uptake by plants was studied after 22 years of continuous cultivation in an annual crop rotation involving maize (Zea mays L.) and wheat (Triticum aestivum L.). Soil samples were collected to a depth of 1.2 m from the 0-0. 15, 0.15-0.30, 0.30-0.45, 0.45-0.60, 0.60-0.90 and 0.90-1.20 m layers and analysed for alkaline KMnO 4 -oxidisable N (available N) and mineral N . The continuous addition of increasing levels of N resulted in an increase in N content, whereas the combined application of N, P and K caused a decline in its availability. Mineral N (2 M KCl-extractable NH 4 -N and NO 3 -N) was the lowest in the N 120 P 35 K 33.2 treatment plot. The available N and NH 4 -N decreased with increasing soil depth. However, variations in NO 3 -N concentration due to differential rates of fertilizer application were observed only to a depth of 0.45 m. This effect was more pronounced in the N 180 P 17.5 K 33.2 plot. Regression equations were used to predict N uptake by wheat using the N status in different soil layers as independent variables. Multiple regression analysis indicated that the predictability of the relationship between N uptake and available N improved considerably when its status to a soil depth of 0.45 m was included. In the case of NH 4 -N, a noticeable increase in the coefficient of determination (R 2 ) occurred to a depth of 0.90 m. The R 2 value of NO 3 -N with the N uptake by wheat was quite low in the top layers (to a depth of 0.30 m). However, an increase in the R 2 value was observed when lower depths (beyond 0.30 m) were included in the regression analysis, suggesting that the inclusion of subsoil N status is important to achieve better and profitable N supply systems in crop production.Key words: soil depth, available and mineral N, fertilizer addition, N uptake
IntroductionNitrogen (N) is a major plant nutrient required for high yields of most agricultural crops. However, the management of nitrogenous fertilizer for crop production remains a difficult task due to the numerous transformation and loss R. K. SETIA et al.Acta Agronomica Hungarica, 54, 2006 488 mechanisms, such as NH 3 volatilization, nitrification following denitrification, chemical and microbial fixation, leaching and runoff (Hussain et al., 2003). Even N applied on the soil surface becomes distributed within the soil profile with subsequent irrigations (Asadi et al., 2002). The nitrate form of N (NO 3 -N) is water-soluble and susceptible to transport to the groundwater, causing the degradation of aquifer water quality in the soils of the Indo-Gangetic plains (Singh et al., 1995a). The rise in the NO 3 -N concentration in the groundwater of Punjab with the increased use of fertilizer was also reported by Singh et al. (1991). On the other hand, some part of the ammoni...