Mouthwash Containing
            <i>Croton Doctoris</i>
            Essential Oil:
            <i>in Vitro</i>
            Study Using a Validated Model of Caries Induction

Albuquerque, Yasmin Etienne; Danelon, Marcelle; Salvador, Marcos José; Koga‐Ito, Cristiane Yumi; Delbem, Alberto Carlos Botazzo; Ramírez-Rueda, Román Yesid; Gontijo, Aline Vidal Lacerda; Brighenti, Fernanda Lourenção

doi:10.2217/fmb-2017-0209

Cited by 15 publications

(15 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to their antimicrobial effects, essential oils may be used to prevent dental caries. There are studies in the literature about the effect of essential oil-containing mouthrinses and mouthwashes against different oral bacteria [31,32,33]. There is only one study that examined the effect of the Thymus vulgaris essential oil against Streptococcus mutans , evaluating the colour, odour, and general appearance of a test toothpaste containing Thymus vulgaris [32].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activities of Herbal Toothpastes Combined with Essential Oils against Streptococcus mutans

et al. 2019

View full text Add to dashboard Cite

In recent years, people have become more conscious about the side-effects of fluoride toothpastes and herbal products have drawn attention as alternatives in the struggle against caries. Studies have focused on the benefits of essential oils obtained from herbs because of their antibacterial effects. The aim of this study was to evaluate and compare the antibacterial activity of Origanum dubium and Cinnamomum cassia oils combined with herbal toothpastes against Streptococcus mutans. The antibacterial activity of the test materials was determined using the agar well diffusion method before and after the addition of essential oils. We tested the efficacy of Splat Organic and Splat Biocalcium against S. mutans (12 mm and 11 mm, respectively) doubled in combination with Origanum dubium (23 mm for both toothpastes) and tripled with Cinnamomum cassia (38 mm and 36 mm, respectively). Jack N’ Jill toothpaste, which did not initially show any antibacterial effect, exhibited the largest inhibition zones after the addition of the essential oils (38 mm for Origanum dubium and 39 mm for Cinnamomum cassia). The results of this study pointed out that herbal toothpastes exhibit statistically higher antibacterial activity against Streptococcus mutans (p < 0.05) than their initial forms after the addition of essential oils.

show abstract

Section: Introductionmentioning

confidence: 99%

Antibacterial Activities of Herbal Toothpastes Combined with Essential Oils against Streptococcus mutans

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Despite the need of clinical studies to confirm the efficacy of and the reduction in the side effects of this association, it is important to highlight that the biofilm growth model used in this study is based on a simple and effective method to study the effect of treatments against dental caries with important characteristics for the developing of oral biofilms [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms

et al. 2022

View full text Add to dashboard Cite

The aim of this study was to evaluate the effect of the Cymbopogon citratus essential oil and its association with chlorhexidine on cariogenic microcosm biofilm composition and acidogenicity. Minimum inhibitory and bactericide concentrations from the essential oil and chlorhexidine were determined by broth microdilution assay. Microcosms (polymicrobial) biofilms were produced on glass coverslips, using inoculum from human saliva in McBain culture medium (0.5% sucrose exposure for 6 h/day) for 3 days in 24-well plates. The biofilms were treated twice a day and their composition was evaluated by microorganism quantification. The acidogenicity was evaluated by measuring the pH of the spent culture medium in contact with the biofilm. Overall, the association of C. citratus and chlorhexidine reduced total bacterial counts and aciduric bacteria (maximum reduction of 3.55 log UFC/mL) in microcosm biofilms. This group also presented the lowest acidogenicity even when exposed to sucrose-containing medium. C. citratus essential oil increases the effect of digluconate chlorhexidine on microcosm biofilms. Based on these findings, this study can contribute to the development of new formulations that might allow for the use of mouthwashes for a shorter period, which may reduce undesirable effects and increase patient compliance to the treatment.

show abstract

“…The results of the present study showed that interactions between S. mutans and A. naeslundii occur during early stages of biofilm formation, leading to a decrease in lactic acid production and to a higher resistance of S. mutans to chlorhexidine exposure. Furthermore, the use of the active adherence model [41,42] allows biofilm formation only with the cells able to adhere to the substrate, which prevents microorganism deposition by the action of gravity. This is a way to reduce limitations related to static biofilm growth and to mimic the oral milieu.…”

Section: Discussionmentioning

confidence: 99%

“…Biofilms were formed on sterilized hydroxyapatite disks [40] using an active adherence model (based on Exterkate et al [41] and Albuquerque et al [42]). Three culture conditions were used: (1) S. mutans single-species biofilms, (2) A. naeslundii single-species biofilms and (3) dual-species biofilms of S. mutans and A. naeslundii.…”

Section: Characterization Of Single-and Dual-species Biofilmsmentioning

confidence: 99%

See 1 more Smart Citation

Streptococcus mutans and Actinomyces naeslundii Interaction in Dual-Species Biofilm

et al. 2020

Self Cite

View full text Add to dashboard Cite

The study of bacterial interaction between Streptococcus mutans and Actinomyces naeslundii may disclose important features of biofilm interspecies relationships. The aim of this study was to characterize—with an emphasis on biofilm formation and composition and metabolic activity—single- and dual-species biofilms of S. mutans or A. naeslundii, and to use a drip flow reactor (DFR) to evaluate biofilm stress responses to 0.2% chlorhexidine diacetate (CHX). Single- and dual-species biofilms were grown for 24 h. The following factors were evaluated: cell viability, biomass and total proteins in the extracellular matrix, 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide—“XTT”—reduction and lactic acid production. To evaluate stress response, biofilms were grown in DFR. Biofilms were treated with CHX or 0.9% sodium chloride (NaCl; control). Biofilms were plated for viability assessment. Confocal laser-scanning microscopy (CLSM) was also performed. Data analysis was carried out at 5% significance level. S. mutans viability and lactic acid production in dual-species biofilms were significantly reduced. S. mutans showed a higher resistance to CHX in dual-species biofilms. Total protein content, biomass and XTT reduction showed no significant differences between single- and dual-species biofilms. CLSM images showed the formation of large clusters in dual-species biofilms. In conclusion, dual-species biofilms reduced S. mutans viability and lactic acid production and increased S. mutans’ resistance to chlorhexidine.

show abstract

Mouthwash Containing Croton Doctoris Essential Oil: in Vitro Study Using a Validated Model of Caries Induction

Abstract: The experimental mouthwash showed antimicrobial activity against polymicrobial biofilms and reduced enamel demineralization.

Cited by 15 publications

References 41 publications

Antibacterial Activities of Herbal Toothpastes Combined with Essential Oils against Streptococcus mutans

Antibacterial Activities of Herbal Toothpastes Combined with Essential Oils against Streptococcus mutans

Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms

Streptococcus mutans and Actinomyces naeslundii Interaction in Dual-Species Biofilm

Contact Info

Product

Resources

About