MouseBytes, an open-access high-throughput pipeline and database for rodent touchscreen-based cognitive assessment

Beraldo, Flávio H.; Palmer, Daniel D.; Memar, Sara; Wasserman, David; Lee, Wai Jane V.; Liang, Shuai; Creighton, Samantha D.; Kolisnyk, Benjamin; Cowan, Matthew F.; Mels, Justin; Masood, Talal; Fodor, Chris; Al‐Onaizi, Mohammed; Bartha, Robert; Gee, Tom; Saksida, Lisa M.; Bussey, Timothy J.; Strother, Stephen S.; Prado, Marco A. M.; Winters, Boyer D.

doi:10.7554/elife.49630

Cited by 40 publications

(56 citation statements)

References 76 publications

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“…ELISA for Tris-soluble and GHCl insoluble Aβ was performed as described previously [50,54,55] following manufacturer's instructions (Catalog# KHB3441, ThermoFisher).…”

Section: Human Aβ 1-42 Elisamentioning

confidence: 99%

Increased levels of Stress-inducible phosphoprotein-1 accelerates amyloid-β deposition in a mouse model of Alzheimer’s disease

Lackie

Marques-Lopes

Ostapchenko

et al. 2020

acta neuropathol commun

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Molecular chaperones and co-chaperones, which are part of the protein quality control machinery, have been shown to regulate distinct aspects of Alzheimer’s Disease (AD) pathology in multiple ways. Notably, the co-chaperone STI1, which presents increased levels in AD, can protect mammalian neurons from amyloid-β toxicity in vitro and reduced STI1 levels worsen Aβ toxicity in C. elegans. However, whether increased STI1 levels can protect neurons in vivo remains unknown. We determined that overexpression of STI1 and/or Hsp90 protected C. elegans expressing Aβ(3–42) against Aβ-mediated paralysis. Mammalian neurons were also protected by elevated levels of endogenous STI1 in vitro, and this effect was mainly due to extracellular STI1. Surprisingly, in the 5xFAD mouse model of AD, by overexpressing STI1, we find increased amyloid burden, which amplifies neurotoxicity and worsens spatial memory deficits in these mutants. Increased levels of STI1 disturbed the expression of Aβ-regulating enzymes (BACE1 and MMP-2), suggesting potential mechanisms by which amyloid burden is increased in mice. Notably, we observed that STI1 accumulates in dense-core AD plaques in both 5xFAD mice and human brain tissue. Our findings suggest that elevated levels of STI1 contribute to Aβ accumulation, and that STI1 is deposited in AD plaques in mice and humans. We conclude that despite the protective effects of STI1 in C. elegans and in mammalian cultured neurons, in vivo, the predominant effect of elevated STI1 is deleterious in AD.

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“…ELISA for Tris-soluble and GHCl insoluble Aβ was performed as described previously [50,54,55] following manufacturer's instructions (Catalog# KHB3441, ThermoFisher).…”

Section: Human Aβ 1-42 Elisamentioning

confidence: 99%

Increased levels of Stress-inducible phosphoprotein-1 accelerates amyloid-β deposition in a mouse model of Alzheimer’s disease

Lackie

Marques-Lopes

Ostapchenko

et al. 2020

acta neuropathol commun

Self Cite

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“…First, the standardized methodology, data collection and extraction of touchscreen technology data across different facilities allowed for the creation of the open science data repository https://www.mousebytes.ca/home 45 . MouseBytes allows touchscreen scientist to upload and share their raw data files, which they can choose to leave private until publication or have their data public.…”

Section: The Need For Open Science Initiatives: Building a Touchscreementioning

confidence: 99%

“…Instead of having to rerun all touchscreen experiments in different mouse models, researchers can simply download the data and reanalyze it using different statistical analyses saving time, money and animal lives. Additionally, https://www.mousebytes.ca/home 45 allows researchers to visualize graphically the data online and can easily tailor comparisons based on interests: sex, age, genotype, location of research facility, etc., all in a matter of a few computer clicks. Currently, there are 1341 mice registered in the database representing data collected from three different countries: Canada, United Kingdom and Australia.…”

Section: The Need For Open Science Initiatives: Building a Touchscreementioning

confidence: 99%

“…In addition to https://www.mousebytes.ca/home 45 and https://touchscreencognition.org/, the International Society for Neurochemistry (ISN) and BrainsCAN provided funding to host the first international touchscreen symposium, new frontiers in cognitive testing using touchscreen technology on 5, 6 June 2019 at Western University, London, ON, Canada. Western University is not only the location of world‐renown rodent cognitive touchscreen testing experts, Dr Tim Bussey and Dr Lisa Saksida, but also the home of the largest rodent touchscreen facility; therefore, Western was the ideal location for the first touchscreen symposium.…”

Section: The Need For Open Science Initiatives: Building a Touchscreementioning

confidence: 99%

“…Highlights of the symposium included: opening remarks by Dr Tim Bussey describing the history of the development of the rodent touchscreens, topics from speakers spanning various cognitive domains (from learning and memory to executive function), neurotechnology (from fiber photometry data to miniscopes) and various psychiatric and disease models (including addiction, Fragile X syndrome, autism spectrum disorder, Huntington's disease, Parkinson's disease, etc.). It also provided an ideal moment to share information about the aims of both https://www.mousebytes.ca/home 45 and https://touchscreencognition.org/, allowing attendees to log onto the website and provide feedback on the Rodent Cognition Core's open science initiatives. Many discussions and data sharing that began during the symposium were expanded on these online open science platforms.…”

Section: The Need For Open Science Initiatives: Building a Touchscreementioning

confidence: 99%

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Critical mass: The rise of a touchscreen technology community for rodent cognitive testing

Dumont

Salewski

Beraldo

2020

Genes Brain and Behavior

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The rise in the number of users and institutions utilizing the rodent touchscreen technology for cognitive testing over the past decade has prompted the need for knowledge mobilization and community building. To address the needs of the growing touchscreen community, the first international touchscreen symposium was hosted at Western University. Attendees from around the world attended talks from expert neuroscientists using touchscreens to examine a vast array of questions regarding cognition and the nervous system. In addition to the symposium, a subset of attendees was invited to partake in a hands‐on training course where they received touchscreen training covering both hardware and software components. Beyond the two touchscreen events, virtual platforms have been developed to further support touchscreen users: (a) http://Mousebytes.ca, which includes a data repository of rodent touchscreen tasks, and (b) http://touchscreencognition.org, an online community with numerous training and community resources, perhaps most notably a forum where members can ask and answer questions. The advantages of the rodent touchscreen technology for cognitive neuroscience research has allowed neuroscientists from diverse backgrounds to test specific cognitive processes using well‐validated and standardized apparatus, contributing to its rise in popularity and its relevance to modern neuroscience research. The commitment of the touchscreen community to data, task development and information sharing not only ensures an expansive future of the use of rodent touchscreen technology but additionally, quality research that will increase translation from preclinical studies to clinical successes.

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Evaluating Sequential Response Learning in the Rodent Operant Touchscreen System

et al. 2021

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Sequential and cue-directed response learning in rodents have been previously shown to depend on intact striatal signaling. In particular, these behaviors rely on striatal dopamine and acetylcholine release, with an impairment of sequential response learning evident in animal models with alterations in the two systems. Here we provide a protocol for testing sequential response/response chain learning using the rodent touchscreen system. Specifically, the present protocol is designed to implement the heterogeneous sequence task, adapted from Keeler et al. ( 2014), in the rodent touchscreen apparatus. This task has been used previously to assess complex motor learning and response selection in mice. In the following protocol, the task is performed in touchscreen-based automated chambers with five response locations using food reinforcers to maintain performance. The sequence task requires the subject to make five nose pokes to white square stimuli appearing in five different locations sequentially from left to right.

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MouseBytes, an open-access high-throughput pipeline and database for rodent touchscreen-based cognitive assessment

Cited by 40 publications

References 76 publications

Increased levels of Stress-inducible phosphoprotein-1 accelerates amyloid-β deposition in a mouse model of Alzheimer’s disease

Increased levels of Stress-inducible phosphoprotein-1 accelerates amyloid-β deposition in a mouse model of Alzheimer’s disease

Critical mass: The rise of a touchscreen technology community for rodent cognitive testing

Evaluating Sequential Response Learning in the Rodent Operant Touchscreen System

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