Mouse Taste Buds Use Serotonin as a Neurotransmitter

Huang, Yi Jen; Maruyama, Yutaka; Lu, Kuo‐Shyan; Pereira, Elizabeth; Plonsky, Ilya; Baur, John E.; Wu, Dianqing; Roper, Stephen D.

doi:10.1523/jneurosci.4446-04.2005

Cited by 159 publications

(174 citation statements)

References 38 publications

(31 reference statements)

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“…Presynaptic cells release serotonin in response to taste stimulation, and thus the synapses that they form and the postulated second pathway may be aminergic (Huang et al, 2005(Huang et al, , 2007. Presynaptic cells also express other neurotransmitters.…”

Section: Discussionmentioning

confidence: 99%

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Breadth of Tuning and Taste Coding in Mammalian Taste Buds

Tomchik¹,

Berg²,

Kim³

et al. 2007

J. Neurosci.

214

270

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A longstanding question in taste research concerns taste coding and, in particular, how broadly are individual taste bud cells tuned to taste qualities (sweet, bitter, umami, salty, and sour). Taste bud cells express G-protein-coupled receptors for sweet, bitter, or umami tastes but not in combination. However, responses to multiple taste qualities have been recorded in individual taste cells. We and others have shown previously there are two classes of taste bud cells directly involved in gustatory signaling: "receptor" (type II) cells that detect and transduce sweet, bitter, and umami compounds, and "presynaptic" (type III) cells. We hypothesize that receptor cells transmit their signals to presynaptic cells. This communication between taste cells could represent a potential convergence of taste information in the taste bud, resulting in taste cells that would respond broadly to multiple taste stimuli. We tested this hypothesis using calcium imaging in a lingual slice preparation. Here, we show that receptor cells are indeed narrowly tuned: 82% responded to only one taste stimulus. In contrast, presynaptic cells are broadly tuned: 83% responded to two or more different taste qualities. Receptor cells responded to bitter, sweet, or umami stimuli but rarely to sour or salty stimuli. Presynaptic cells responded to all taste qualities, including sour and salty. These data further elaborate functional differences between receptor cells and presynaptic cells, provide strong evidence for communication within the taste bud, and resolve the paradox of broad taste cell tuning despite mutually exclusive receptor expression.

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Section: Discussionmentioning

confidence: 99%

“…Parenthetically, presynaptic taste cells are serotonergic and release 5-HT during stimulation (Yee et al, 2001;Huang et al, 2005Huang et al, , 2007DeFazio et al, 2006). Thus, we also immunostained taste buds from GAD-GFP mice for the presence of 5-HT (Fig.…”

Section: Using Transgenic Mice To Identify Receptor Cells and Presynamentioning

confidence: 99%

Breadth of Tuning and Taste Coding in Mammalian Taste Buds

Tomchik¹,

Berg²,

Kim³

et al. 2007

J. Neurosci.

214

270

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“…Roper, 2006). It is known that 5-HT release from taste buds is not dependent on calcium influx but on store release (Huang et al, 2005). (4) We propose a serotonergic feedback from presynaptic to receptor cells where 5-HT acts via 5-HT 1A receptors (Delay et al, 1997;Herness and Chen, 1997;Herness and Chen, 2000;Kaya et al, 2004) to modulate bitter and sweet signaling.…”

Section: Effects Of Monoamine Manipulation On Taste Perceptionmentioning

confidence: 98%

“…In addition to the localization of 5-HT and its synthetic enzymes, transporters, and receptors in taste cells (Kim and Roper, 1995;Delay et al, 1997;Ren et al, 1999;DeFazio et al, 2006) and its release on stimulation of taste cells (Huang et al, 2005), functional work using zinc-deficient mice (which inhibits the formation of 5-HT in taste cells) showed that the animals had decreased sensitivity to bitter tastes (Kondo et al, 1987). This suggests that 5-HT signaling in taste buds is fundamental to normal taste.…”

Section: -Ht Na and Tastementioning

confidence: 99%

“…This modulation of monoamine function may have an impact on taste perception, and this may be the basis for altered taste in affective disorders. The strongest evidence for an involvement of 5-HT and NA in taste signaling is at the level of the taste bud: taste cells from many species express 5-HT (Nada and Hirata, 1975;Kim and Roper, 1995;Clapp et al, 2004), its synthetic enzymes (DeFazio et al, 2006), and its receptors (Delay et al, 1997), and both take up 5-HT (Nagai et al, 1998) and release it in response to stimulation with tastants (Huang et al, 2005). 5-HT and NA affect taste cell excitability by altering ion channel function (Herness and Chen, 1997;Herness and Chen, 2000;Herness et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Human Taste Thresholds Are Modulated by Serotonin and Noradrenaline

Heath

Melichar²,

Nutt³

et al. 2006

J. Neurosci.

172

151

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Circumstances in which serotonin (5-HT) and noradrenaline (NA) are altered, such as in anxiety or depression, are associated with taste disturbances, indicating the importance of these transmitters in the determination of taste thresholds in health and disease. In this study, we show for the first time that human taste thresholds are plastic and are lowered by modulation of systemic monoamines. Measurement of taste function in healthy humans before and after a 5-HT reuptake inhibitor, NA reuptake inhibitor, or placebo showed that enhancing 5-HT significantly reduced the sucrose taste threshold by 27% and the quinine taste threshold by 53%. In contrast, enhancing NA significantly reduced bitter taste threshold by 39% and sour threshold by 22%. In addition, the anxiety level was positively correlated with bitter and salt taste thresholds. We show that 5-HT and NA participate in setting taste thresholds, that human taste in normal healthy subjects is plastic, and that modulation of these neurotransmitters has distinct effects on different taste modalities. We present a model to explain these findings. In addition, we show that the general anxiety level is directly related to taste perception, suggesting that altered taste and appetite seen in affective disorders may reflect an actual change in the gustatory system.

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Anatomy of the Tongue and Taste Buds

Witt

Reutter

2015

Handbook of Olfaction and Gustation

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Mouse Taste Buds Use Serotonin as a Neurotransmitter

Cited by 159 publications

References 38 publications

Breadth of Tuning and Taste Coding in Mammalian Taste Buds

Breadth of Tuning and Taste Coding in Mammalian Taste Buds

Human Taste Thresholds Are Modulated by Serotonin and Noradrenaline

Anatomy of the Tongue and Taste Buds

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