Mouse strain differences in the induction of micronuclei by polycyclic aromatic hydrocarbons

Sato, Seiichi; Kitajima, Hirono; Konishi, Sumie; Takizawa, Haruo; Inui, Naomichi

doi:10.1016/0165-7992(87)90054-6

Cited by 22 publications

(7 citation statements)

References 11 publications

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“…When compared to the outbred OF1 and NMRI mice, and the inbred AKR, C57B116, and BALB/c mice, the MS/Ae mouse was again clearly the most responsive [Aeschbacher, 19861. In the Aeschbacher [ 19861 study, BALB/c was the second most responsive mouse stock. In comparison studies with BALB/c where the MS/Ae mouse was absent, BALB/c generally exhibited the highest sensitivity, although not for all chemicals tested [Sato et al, 1987[Sato et al, , 1990. Sat0 et al…”

Section: Swiss Webstermentioning

confidence: 99%

Bone marrow micronucleus assay: A review of the mouse stocks used and their published mean spontaneous micronucleus frequencies

Salamone

Mavournin

1994

Environ and Mol Mutagen

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We have examined published negative control data from 581 papers on micronucleated bone marrow polychromatic erythrocytes (mnPCE) for differences in mean frequency and the frequency distribution profile among the mouse stocks used with the bone marrow micronucleus assay. For the 55 mouse stocks with published micronucleus assay data, the overall mean frequency is 1.95 mnPCE/1,000 PCE (1.95 mnPCE/1,000); for the 13 stocks most commonly used in the assay, it is 1.88 mnPCE/1,000. During the last 5 years, the mnPCE rate for these 13 major stocks has been 1.74 mnPCE/1,000. This current mean frequency is a substantial decrease from the mean of 3.07 mnPCE/1,000 observed for these 13 stocks for data published prior to 1981. Of the major stocks, the highest mean mnPCE negative control frequencies were observed for MS/Ae > BALB/c > C57Bl/6, and the lowest for CD-1 < Swiss Webster. We note that hybrid mouse stocks appear to have lower and less variable negative control frequencies than either of their parent strains and that the negative control frequency for some progeny stocks have diverged significantly from that of the parent stocks. Overall mean negative control frequencies appear to be correlated with breadth of the frequency distribution profile of published mean negative control values. Furthermore, a possible correlation between negative control frequency in the micronucleus assay and sensitivity to clastogens of different mouse strains may be indicated. The databases generated here allow us to define a range of norms for both the historical mean frequency and individual experimental mean frequencies for most stocks, but in particular, for the more commonly used mouse stocks. Our analysis, for the most part, bears out the recommendation of the first Gene-Tox Report on the micronucleus assay that the historical negative control frequency for a mouse stock should fall between 1 and 3 mnPCE/1,000. Eighty-six percent of the most commonly used mouse stocks have historical mean frequencies within this range. Though individual experimental mean values would not necessarily be expected to fall within the 1-3.00 mnPCE/1,000 range, 65.3% of the 2,327 published negative control values do, and 83.5% are < 3 mnPCE/1,000. The frequency with which an individual experimental mean value lies outside the 1.00 to 3.00 mnPCE/1,000 range differs among stocks and appears related to the mouse mean frequency. We suggest that the recommended range for historical mean frequency be extended slightly, to approximately 3.4 mnPCE/1,000, to accommodate some commonly used strains with overall mean negative control frequencies just above 3.00 mnPCE/1,000.(ABSTRACT TRUNCATED AT 400 WORDS)

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Section: Swiss Webstermentioning

confidence: 99%

Bone marrow micronucleus assay: A review of the mouse stocks used and their published mean spontaneous micronucleus frequencies

Salamone

Mavournin

1994

Environ and Mol Mutagen

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“…PAHs are formed during the incomplete burning of fossil fuel garbage and other organic substances such as tobacco or charbroiled meat. Polycyclic aromatic hydrocarbons are chemically inert and may require activation by metabolic enzymes to form active metabolites that interact with deoxyribonucleic acid (DNA) to initiate cell transformation (Mane, Purnell, & Hsu, 1990; Sato, Kitajima, Konishi, Takizawa, & Inui, 1987). This multi‐step activation cascade suggests that a number of intervening conditions or predispositions may affect the extent to which environmental carcinogens cause abnormal cell growth.…”

Section: Introductionmentioning

confidence: 99%

Psychological stress accelerates the onset of tumour formation and alters the type and location of tumours in a DMBA mouse carcinogenesis model

et al. 2011

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Psychological stress is recognized as a factor that contributes to increased susceptibility to a number of diseases, including cancer. Psychological stress, via release of chemical mediators, can induce long‐term changes in the organism resulting in an altered responsiveness of the organism to external carcinogens. The present investigation sought to evaluate the impact of stress on polycyclic aromatic hydrocarbon (PAH)‐induced carcinogenesis. We utilized a repetitive restraint stress mouse model and the model PAH, 7,12‐dimethylbenz[a]anthracene (DMBA). Restraint stress was applied three times a week for 6 weeks and DMBA was administered intra‐gastrically once a week for 6 weeks and formation of skin, mammary and ovarian tumours was examined at 26 weeks by pathological analyses. The results indicate that stress accelerates the onset of tumour formation produced in response to DMBA and significantly influences the type and location of tumours. Taken together, these results demonstrate that stress enhances the carcinogenicity of DMBA. These results clearly indicate the need for further characterization of the impact of stress on carcinogen metabolism in oncology. Copyright © 2010 John Wiley & Sons, Ltd.

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“…The incidences of the development of micronuclei, DNA-damaged cells and skin tumors were markedly increased in the AHH-inducible mice (BALB/c and C57BL/6), as compared with the AHH-noninducible mice (DBA/2). 22,23) These results suggest that carcinogenicity induced by DMBA in mice correlates well with the induction of a micronucleus.…”

Section: The Induction Of a Micronucleus By Carcinogenic Substancesmentioning

confidence: 56%

Short-Term Screening Method for the Prediction of Carcinogenicity of Chemical Substances. Current Status and Problems of an in vivo Rodent Micronucleus Assay.

Sato

Tomita

2001

JOURNAL OF HEALTH SCIENCE

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Various short-term screening methods have been developed to detect mutagenic/carcinogenic substances. They have played important roles not only in screening suspected chemicals but in studying the mechanisms of mutagenesis/carcinogenesis, and have provided useful information for assessing the genetic effects of chemicals on humans. The micronucleus assay is an in vivo mammalian methods, which has been widely used for screening the genotoxic potency of chemical substances. By this assay, the genotoxicity of chemicals, including clastogens and spindle poisons, have been evaluated using immature bone marrow erythrocytes in mice. The use of immature erythrocytes in circulating peripheral blood instead of in bone marrow of mice has been developed in recent years and it has been determined that it gives results as relevant as those of bone marrow cells in mice. Using the peripheral blood of mice has made the micronucleus assay method more practical and useful. Recently, rats have been identified as an acceptable species for this assay, and assays using the peripheral blood of rats are often carried out concomitantly with general toxicity or carcinogenic studies. However, it must be noted that the micronucleus assays have some limitations. The activity of substances which are metabolized rapidly in mice or rats, for example, may hardly be detected by the micronucleus assay; metabolic features of the substances may affect the assay results greatly. In this review, the current status of the micronucleus assay is discussed as a short-term screening method in the context of its usefulness and its limitations.

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Mouse strain differences in the induction of micronuclei by polycyclic aromatic hydrocarbons

Cited by 22 publications

References 11 publications

Bone marrow micronucleus assay: A review of the mouse stocks used and their published mean spontaneous micronucleus frequencies

Bone marrow micronucleus assay: A review of the mouse stocks used and their published mean spontaneous micronucleus frequencies

Psychological stress accelerates the onset of tumour formation and alters the type and location of tumours in a DMBA mouse carcinogenesis model

Short-Term Screening Method for the Prediction of Carcinogenicity of Chemical Substances. Current Status and Problems of an in vivo Rodent Micronucleus Assay.

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