Mouse splenic and bone marrow cell populations that express high-affinity Fc epsilon receptors and produce interleukin 4 are highly enriched in basophils.

Seder, Robert A.; Paul, William E.; Dvorak, Ann M.; Sharkis, Saul J.; Kagey‐Sobotka, Anne; Niv, Yaffa; Finkelman, Fred D.; Barbieri, Sebastiano; Galli, Stephen J.; Plaut, Marshall

doi:10.1073/pnas.88.7.2835

Cited by 234 publications

(141 citation statements)

References 38 publications

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“…IL-4-induced IFN-␥ production in PDCs was also observed in C57BL/6 mice (data not shown), suggesting that this phenomenon is a general one observed beyond strain differences. Because IL-4 is produced in an early phase in immune responses by NK T cells (44) and/or basophils (45,46), the IL-4-induced IFN-␥ production by PDCs may function in the negativefeedback regulation against a Th2-type deviation in an early phase of immune responses. In this regard, de Heer et al (14) have demonstrated recently that PDCs inhibit typical Th2 responses such as IgE production and allergic airway inflammation.…”

Section: Discussionmentioning

confidence: 99%

Murine Plasmacytoid Dendritic Cells Produce IFN-γ upon IL-4 Stimulation

Suto

Nakajima

Tokumasa

et al. 2005

The Journal of Immunology

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IL-4 plays a key role in inducing IL-4 production in CD4+ T cells, functioning as an important determinant for Th2 cell differentiation. We show here that IL-4 induces IFN-γ production in B220+ plasmacytoid dendritic cells (PDCs). By searching for cell populations that produce IFN-γ upon IL-4 stimulation, we found that PDCs were a major IFN-γ-producing cell upon IL-4 stimulation in wild-type and Rag-2−/− splenocytes. Isolated PDCs, but not CD11b+ DCs or CD8+ DCs, produced IFN-γ upon IL-4 stimulation. In vivo, the depletion of PDCs by anti-Ly6G/C Ab prevented IFN-γ production induced by IL-4 administration. We also found that IL-4 induced IFN-γ production, but not IL-12 or IFN-α production, in PDCs and also strongly enhanced CpG oligodeoxynucleotide-induced IFN-γ production, but not CpG oligodeoxynucleotide-induced IL-12 or IFN-α production. However, IL-4 did not induce IFN-γ production in Stat6−/− PDCs. Moreover, IL-4 induced Stat4 expression in PDCs through a Stat6-dependent mechanism, and only the Stat4-expressing PDCs produced IFN-γ. Furthermore, IL-4 did not induce IFN-γ production in Stat4−/− PDCs. These results indicate that PDCs preferentially produce IFN-γ upon IL-4 stimulation by Stat6- and Stat4-dependent mechanisms.

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Section: Discussionmentioning

confidence: 99%

Murine Plasmacytoid Dendritic Cells Produce IFN-γ upon IL-4 Stimulation

Suto

Nakajima

Tokumasa

et al. 2005

The Journal of Immunology

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“…Upon receptor cross-linking, activated basophils rapidly release effector molecules including histamine and proteases from cytoplasmic granules. They further produce various proinflammatory lipid mediators, chemokines, and the Th2-associated cytokines IL-4, IL-5, and IL-13 (9)(10)(11). Over the past years several genetic mouse models have been used to elucidate basophil functions in vivo.…”

mentioning

confidence: 99%

Basophil-mediated protection against gastrointestinal helminths requires IgE-induced cytokine secretion

Schwartz

Turqueti-Neves

Hartmann

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Significance Gastrointestinal worms (helminths) infect more than 2 billion people, and vaccines are not yet available. Helminths elicit a type 2 immune response characterized by high serum IgE levels and increased numbers of IL-4– or IL-13–secreting effector cells including Th2 cells, eosinophils, basophils, and type 2 innate lymphoid cells. We determined the mechanism by which basophils contribute to protection against secondary infections with gastrointestinal helminths. Here we demonstrate that basophils are recruited into the small intestine of infected mice and orchestrate the local type 2 immune response in this tissue. Basophil-mediated protection required the presence of IgE and the expression of activating Fc receptors and IL-4/IL-13 in basophils. These findings could help the development of new vaccination strategies against helminths.

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“…Recent studies indicated that basophils regulate Th1/Th2 homeostasis (5,6) and humoral immunity (7). Basophils produce large amounts of IL-4, a key cytokine in Th2 skewing, in response to various stimuli, including IL-3, the protease allergen papain, and cross-linking of surfacebound IgE molecules (8,9). In addition, basophils have been shown to express MHC class II and costimulatory molecules and to function as APCs (10)(11)(12), although the role of basophils as APCs in vivo is still controversial (13)(14)(15)(16).…”

mentioning

confidence: 99%

Identification of Semaphorin 4B as a Negative Regulator of Basophil-Mediated Immune Responses

Nakagawa

Takamatsu

Okuno

et al. 2011

The Journal of Immunology

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Basophils are strong mediators of Th2 responses during helminthic infections. Recently, basophils were shown to function as APCs and promote both Th2 skewing and humoral memory responses. However, the mechanisms that regulate basophils are still unclear. In this article, we show that a class IV semaphorin, Sema4B, negatively regulates basophil functions through T cell–basophil contacts. In a screen to identify semaphorins that function in the immune system, we determined that Sema4B is expressed in T and B cells. Interestingly, Sema4B−/− mice had considerably increased serum IgE levels despite normal lymphocyte and dendritic cell functions. Recombinant Sema4B significantly inhibited IL-4 and IL-6 production from basophils in response to various stimuli, including IL-3, papain, and FcεRI cross-linking. In addition, T cell-derived Sema4B, which accumulated at contact sites between basophils and CD4+ T cells, suppressed basophil-mediated Th2 skewing, suggesting that Sema4B regulates basophil responses through cognate cell–cell contacts. Furthermore, Sema4B−/− mice had enhanced basophil-mediated memory IgE production, which was abolished by treating with an anti-FcεRIα Ab. Collectively, these results indicate that Sema4B negatively regulates basophil-mediated Th2 and humoral memory responses.

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Mouse splenic and bone marrow cell populations that express high-affinity Fc epsilon receptors and produce interleukin 4 are highly enriched in basophils.

Abstract: Splenic and bone marrow cells from normal mice, and from mice that have been polyconally activated by injection of anti-IgD antibody, contain cells that produce interleukin 4 (IL4) in response to crosslinkage of Fc, receptors

Cited by 234 publications

References 38 publications

Murine Plasmacytoid Dendritic Cells Produce IFN-γ upon IL-4 Stimulation

Murine Plasmacytoid Dendritic Cells Produce IFN-γ upon IL-4 Stimulation

Basophil-mediated protection against gastrointestinal helminths requires IgE-induced cytokine secretion

Identification of Semaphorin 4B as a Negative Regulator of Basophil-Mediated Immune Responses

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