Mouse Skin Graft Prolongation With Donor Strain Bone Marrow and Antilymphocyte Serum

Potent Skin Allograft Survival Prolongation Using a Committed Progenitor Fraction of Bone Marrow in Mice

“…De Fazio et al (26) performed studies evaluating skin graft survival in mice given fractionated BM by using discontinuous Percoll gradient centrifugation. They isolated a light-density BM fraction that was as effective as whole BM in prolonging skin graft survival in ALS-conditioned mice and dogs (27,28).…”

“…When a dose of 150ϫ10 6 donor BM cells was used, the median skin graft survival in mice treated with ALS alone, Sir alone, BM alone, ALS and BM, Sir and BM, and ALS and Sir was 21 (17)(18)(19)(20)(21)(22)(23), 18 (16 -22), 9 (8 -11), 17 (10 -23), 20 (18 -22), and 26 (23)(24)(25)(26)(27)(28)(29)(30) days, respectively. The median graft survival in mice treated with ALS, Sir, and BM (25 M) and ALS, Sir, and BM (150 M) was 61 (57-72) and 190 (158 -456) days, respectively (Fig.…”

Characterization of in vitro antimurine thymocyte globulin–induced regulatory T cells that inhibit graft-versus-host disease in vivo

“…Thymoglobulin has been shown to recognize a variety of T-cell as well as non-T-cell surface antigens, [3][4][5][6][7] but it is also likely to recognize additional surface antigens not yet examined or known. Murine counterpart reagents such as murine antithymocyte or antilymphocyte reagents also deplete T cells in mice and show protective effects in diabetes 8,9 and graft-versus-host disease 10 models and prevent rejection of pancreas allografts, 11 islet xenografts, 12 skin allografts cotransplanted with donor bone marrow, 13 and allogeneic heart transplants (S.R.N., J.M.W., and Lan Gao, unpublished data, September 2004). Depletion of T cells has been thought to be the primary mechanism of the protection observed in both human transplant patients and animal models 14 ; however, given the polyclonal nature of antithymocyte globulins, additional mechanisms of immunosuppression have been suggested.…”

Alloantigen persistence in induction and maintenance of transplantation tolerance.

“…Infusion of allogeneic lymphohematopoietic cells into immunologically immature or immunosuppressed recipients may under certain circumstances lead to the induction of permanent tolerance to allografts, as opposed to the mere prolongation of allograft survival obtained in unreconstituted hosts (1)(2)(3)(4). We have studied the mechanism underlying transplantation tolerance induced by total lymphoid irradiation (TLI).…”