Mouse ornithine decarboxylase gene: cloning, structure, and expression.

Abstract

We used molecular cloning to isolate a functional gene for mouse ornithine decarboxylase (OrnDCase; L-ornithine carboxy-lyase, EC 4.1

Regulation of mouse ornithine decarboxylase activity by cell growth, serum and tetradecanoyl phorbol acetate is governed primarily by sequences within the coding region of the gene

“…The fragment of the ODC gene extends 2.7 kb 5' to the TATA homology and transcription start sites and terminates at its 3' end at a Sal I site within the second exon encoding part of the untranslated leader sequence. We have shown previously that this fragment of the ODC gene is comparable in promoter activity to the Rous sarcoma virus long terminal repeat promoter and more active than the SV40 early region promoter in the transient expression assay using mouse cell recipients [9].…”

“…Induction of ODC activity and mRNA by TPA appears uniformly reduced in transfectants (2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13) fold induction of activity; fig. 3, table 2) as compared to NIH3T3 cells fold induction of activity; fig 3, table 1).…”

“…Figure 2: Structure of the ODC Gene and of Derived Recombinant Constructs. A. pOD12.7 is a genomic ODC clone whose isolation from ODC-overproducing S49 mouse lymphoma cells has been described [9]. The [14].…”

“…To better understand the nature of regulation of ODC activity, we and others have cloned mouse ODC genomic DNA and cDNA from cells in which that gene was selectively amplified [9][10][11][12][13]. We showed the cloned ODC gene to be functional by stably transfecting [9,14] ODC-deficient also produce correctly initiated and sized mouse ODC mRNA transcripts and enzymatically active ODC polypeptide [9].…”

Induction of ornithine decarboxylase by IL-3 is mediated by sequential c-Myc-independent and c-Myc-dependent pathways

“…These reporter constructs contain exon 1, intron 1 and part of exon 2 of the murine ODC gene fused to the CAT reporter gene and either 264 bp or approximately 2.5 Kbp of sequences upstream of the ODC RNA start site, respectively (Brabant et al, 1988). Transfected cells were then divided into two¯asks, and either maintained in the continued absence of IL-3 or were supplemented with puri®ed IL-3 (100 U per ml).…”