Mouse oocytes penetrated by sperm at GV or GVBD stage lose the ablity to fuse with additional spermatozoa

Pyrzyńska, Beata; Maleszewski, Marek; Maluchnik, Darek

doi:10.1017/s0967199400002999

Cited by 18 publications

(11 citation statements)

References 22 publications

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“…Under experimental conditions spermatozoa may also penetrate maturing oocytes, i.e., oocytes that have not yet reached metaphase II (Clarke & Masui 1986, 1987, Pyrzynska et al 1996, Ajduk & Maleszewski 2004). Although it is well known that maturing mouse oocytes react to experimental fertilization or sperm factor injection with attenuated Ca 2C oscillations (lower amplitude and/or number of Ca 2C transients; Carroll et al 1994, Jones et al 1995, Cheung et al 2000, reviewed in Ajduk et al 2008, the downstream effect of such premature fertilization has not been examined.…”

Section: Introductionmentioning

confidence: 99%

Fertilization differently affects the levels of cyclin B1 and M-phase promoting factor activity in maturing and metaphase II mouse oocytes

Ajduk¹,

Ciemerych²,

Nixon³

et al. 2008

Reproduction

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Fertilization affects levels of cyclin B1 and M-phase promoting factor (MPF) activity in maturing and metaphase II mouse oocytes in two distinct ways. In metaphase II oocytes, it leads to a Ca 2C -dependent, continuous degradation of cyclin B1 and inactivation of cyclin dependent kinase (CDC2A)-cyclin B1 complex (MPF). In this paper, we show that neither mono-nor polyspermic fertilization of prometaphase I and metaphase I oocytes triggered degradation of cyclin B1. However, polyspermic fertilization of prometaphase I oocytes led to a transient decrease in MPF activity that lasted for 2 h. The inactivation of MPF in polyspermic prometaphase I oocytes did not depend on the fertilization-induced increase in the cytoplasmic concentration of free Ca 2C ions, but was caused, at least in part, by dephosphorylation of CDC2A at threonine 161 (Thr161). We found that polyspermic fertilization did not affect glutathione levels in prometaphase I oocytes, and concluded that the decrease in MPF activity and dephosphorylation of CDC2A at Thr161 in polyspermic prometaphase I oocytes were not caused by a change in the redox status of the cell induced by an introduction of excessive amount of sperm protamines. Instead, we propose that inactivation of MPF activity in polyspermic maturing oocytes is caused by a change in nucleocytoplasmic ratio that leads to a 'titration' of kinases and phosphatases responsible for keeping MPF in an active state. This idea is supported by the finding that oocytes fused with thymocytes rather than spermatozoa also showed a transient decrease in MPF activity.Reproduction (2008) 136 741-752

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Section: Introductionmentioning

confidence: 99%

Fertilization differently affects the levels of cyclin B1 and M-phase promoting factor activity in maturing and metaphase II mouse oocytes

Ajduk¹,

Ciemerych²,

Nixon³

et al. 2008

Reproduction

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“…However, it is interesting to note that the oocyte plasma membrane, which does not mingle with sperm plasma membrane in ICSI, remains fusible with spermatozoa from the outside for many hours . Perhaps, during normal fertilization, sperm plasma membrane components mingling with the oocyte plasma membrane renders the oocyte non-fusible with additional spermatozoa (Pyrzynska et al 1996;Krukowska et al 1998).…”

Section: How Does Icsi Work?mentioning

confidence: 99%

Gamete manipulation for development: new methods for conception

Yanagimachi

2001

Reprod. Fertil. Dev.

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Mammalian oocytes microsurgically injected with spermatozoa can develop into normal offspring. Apparently the oocyte has the ability to decompose or eliminate such sperm components as the plasma membrane and acrosomal contents, which normally do not enter its cytoplasm. Species in which normal offspring were obtained by direct sperm injection include: human, mouse, rabbit, horse, sheep, cattle, pig, and monkey. In the mouse, normal offspring can also be obtained routinely by the injection of round spermatid nuclei into oocytes. This suggests that all post-meiotic modifications of spermatozoa (spermiogenesis, sperm maturation, capacitation and the acrosome reaction) evolved as processes solely dedicated to delivering male genomes into female gametes. Birth of normal offspring after injection of spermatocytes into maturing or mature oocytes suggests that the mechanisms controlling meiosis of male and female germ cells are similar, if not the same. Spermatozoa do not need to be morphologically normal or alive in the conventional sense to participate in embryo development; as long as they have intact genomes, they are able to produce normal offspring. Chromosomes within the first and second polar bodies can be used as substitutes for female pronuclei for the production of normal offspring. The nuclei of adult somatic cells can be used for production of animals. This procedure, involving introduction of cell nuclei into enucleated oocytes (genomic cloning), is rather inefficient at present. Many obstacles must be overcome before it is accepted as a safe, novel method to reproduce scientifically, medically or economically valuable animals. For human cloning the prime consideration must be the welfare of the child.

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“…Germinal vesicle (GV) stage oocytes have no ability to participate in the early steps of fertilization. Although sperm penetration has been reported in GV oocytes [ 3 ], sperm head remodelling does not progress beyond slight swelling. In many species, an increase in sperm remodelling capability is acquired following a period of in-vitro maturation [ 4 - 8 ].…”

Section: Introductionmentioning

confidence: 99%

Reduced developmental competence of immature, in-vitro matured and postovulatory aged mouse oocytes following IVF and ICSI

Lacham-Kaplan

Trounson

2008

Reprod Biol Endocrinol

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Background: The present study highlights basic physiological differences associated with oocyte maturation and ageing. The study explores the fertilizing capacity and resistance to injury of mouse oocytes at different stages of maturation and ageing following IVF and ICSI. Also, the study examines the developmental competence of embryos obtained from these oocytes. The outcome of the study supports views that the mouse can be a model for human IVF suggesting that utilizing in-vitro matured and failed fertilized oocytes to produce embryos mainly when limited number of oocytes is retrieved in a specific cycle, should be carefully considered.

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Mouse oocytes penetrated by sperm at GV or GVBD stage lose the ablity to fuse with additional spermatozoa

Cited by 18 publications

References 22 publications

Fertilization differently affects the levels of cyclin B1 and M-phase promoting factor activity in maturing and metaphase II mouse oocytes

Fertilization differently affects the levels of cyclin B1 and M-phase promoting factor activity in maturing and metaphase II mouse oocytes

Gamete manipulation for development: new methods for conception

Reduced developmental competence of immature, in-vitro matured and postovulatory aged mouse oocytes following IVF and ICSI

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