Mouse models for abdominal aortic aneurysm

Golledge, Jonathan; Krishna, Smriti Murali; Wang, Yutang

doi:10.1111/bph.15260

Cited by 45 publications

(46 citation statements)

References 158 publications

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“…To explore the possible application of SHEs in mechanical support, we tentatively used three crosslinked SHEs (SHE0.5, SHE1 and SHE2) to limit aneurysm expansion. We chose PPE adventitial application to establish aneurysm model which have similar pathological changes of aneurysm with human 37,38 . We wrapped the pretrimmed SHEs around the aneurysm and achieved in situ seamless sleeving (Supplementary Movie 2).…”

Section: Discussionmentioning

confidence: 99%

Self-healing polyurethane-elastomer with mechanical tunability for multiple biomedical applications in vivo

et al. 2021

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The unique properties of self-healing materials hold great potential in the field of biomedical engineering. Although previous studies have focused on the design and synthesis of self-healing materials, their application in in vivo settings remains limited. Here, we design a series of biodegradable and biocompatible self-healing elastomers (SHEs) with tunable mechanical properties, and apply them to various disease models in vivo, in order to test their reparative potential in multiple tissues and at physiological conditions. We validate the effectiveness of SHEs as promising therapies for aortic aneurysm, nerve coaptation and bone immobilization in three animal models. The data presented here support the translation potential of SHEs in diverse settings, and pave the way for the development of self-healing materials in clinical contexts.

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Section: Discussionmentioning

confidence: 99%

Self-healing polyurethane-elastomer with mechanical tunability for multiple biomedical applications in vivo

et al. 2021

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“…Similar to human AAA, the PPE model allows to create AAA in infrarenal aorta where almost clinical AAAs locate [5,8,12,26,27]. In this study, we provide the evidenced that day 14 after the surgery is sufficient for analyzing aneurysmal histopathologies in AAA biomedical and translational preclinical studies.…”

Section: Discussionmentioning

confidence: 96%

“…With implementation of endovascular aneurysm repair, it is difficult to collect clinical AAA specimens for research purpose [23][24][25]. Animal models have become critically important for studying the pathogenesis and therapies of AAAs [12,[26][27][28]. While experimental AAAs can be induced in several rodents for biomedical and therapeutical studies, the mouse is the most popular used species [12,26,27,29,30].…”

Section: Discussionmentioning

confidence: 99%

“…Animal models have become critically important for studying the pathogenesis and therapies of AAAs [12,[26][27][28]. While experimental AAAs can be induced in several rodents for biomedical and therapeutical studies, the mouse is the most popular used species [12,26,27,29,30]. A previous study, which focuses on spatiotemporal matrix remodeling, pointed out that aortic expansion was slower between 14 and 21 days than earlier, suggesting autostabilization of the AAA in rats [31].…”

Section: Discussionmentioning

confidence: 99%

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Temporal and Quantitative Analysis of Aortic Immunopathologies in Elastase-Induced Mouse Abdominal Aortic Aneurysms

Tian

Chen

Liu

et al. 2021

Journal of Immunology Research

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Objective. Elastase-induced abdominal aortic aneurysm (AAA) model is widely used for aneurysmal pathogenesis and translational research. However, temporal alternations in aneurysmal histologies remain unknown. This study is aimed at analyzing temporal immunopathologies of aneurysmal aorta following experimental AAA induction. Methods. Male C57BL/6J mice at the age of 10-14 weeks received intra-aortic infusion of elastase to induce AAAs. Aortic diameters at the baseline and indicated days after AAA induction were measured, and aortae were collected for histopathological analysis. Results. Aorta diameters increased from 0.52 mm at the baseline levels to 0.99 mm, 1.34 mm, and 1.41 mm at days 7, 14, and 28, respectively, corresponding 90%, 158%, and 171% increases over the baseline level. Average aortic diameters did not differ between days 14 and 28. Severe elastin degradation and smooth muscle cell depletion were found at days 14 and 28 as compared to the baseline and day 7. No difference in the scores of medial elastin and SMC destruction was noted between days 14 and 28. Consistent results were found for leukocyte accumulation, neoangiogenesis, and matrix metalloproteinase expression. Twenty-eight days after AAA induction, all aneurysmal pathologies showed an attenuated trend, although most histopathological parameters did no differ between days 14 and 28. Conclusion. Our data suggest that almost aneurysmal immunohistopathologies reach maximal 14 days following AAA induction. Analysis of day 14 histologies is sufficient for AAA pathogenesis and translational studies in elastase-induced mouse experimental AAAs.

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“…As this procedure is itself associated with a risk of serious perioperative complications, patients with small aneurysms are treated conservatively despite 70% of small aneurysms continuing to grow with an increased risk of rupture. There is clearly an urgent need to develop drug therapies effective at slowing abdominal aortic aneurysm growth, and the review by Golledge et al (2021) describes the commonly used mouse models for abdominal aortic aneurysm that are being used in this research. Another prevalent type of vascular injury that results in significant cardiovascular morbidity and mortality is the rupture of unstable or vulnerable atherosclerotic plaques.…”

Section: Linked Articlesmentioning

confidence: 99%