Mouse Hepatic Cells Support Assembly of Infectious Hepatitis C Virus Particles

Long, Gang; Hiet, Marie–Sophie; Windisch, Marc P.; Lee, Ji–Young; Lohmann, Volker; Bartenschlager, Ralf

doi:10.1053/j.gastro.2011.06.010

Cited by 100 publications

(106 citation statements)

References 37 publications

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“…The nature of the attachment factor for HCV particles of low density was not identified in this study but is likely a molecule that binds VLDL, as deduced from VLDL and apoE competition in infection assays. Thus, in agreement with several previous studies (17,22,84), lipoprotein components provide the viral particle mimics of host-derived ligands for important capture molecules such as, e.g. LDL receptor and SR-BI.…”

Section: Journal Of Biological Chemistrysupporting

confidence: 92%

Characterization of Hepatitis C Virus Particle Subpopulations Reveals Multiple Usage of the Scavenger Receptor BI for Entry Steps

Thi

Granier

Zeisel

et al. 2012

Journal of Biological Chemistry

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Section: Journal Of Biological Chemistrysupporting

confidence: 92%

Characterization of Hepatitis C Virus Particle Subpopulations Reveals Multiple Usage of the Scavenger Receptor BI for Entry Steps

Thi

Granier

Zeisel

et al. 2012

Journal of Biological Chemistry

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109

111

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“…Moreover, several human cells of non-liver origin are well established . Finally, a spectrum of non-human cells have been reported that sustain HCV replication (Zhu, Guo et al 2003;Chang, Cai et al 2006;Uprichard, Chung et al 2006;Long, Hiet et al 2011) (Figure 2). However, it is important to note that RNA-replication is in general lower in these cells, particularly in transient replication assays, com pared to Huh7-derived cell clones.…”

Section: Permissive Host Cellsmentioning

confidence: 99%

Cell Culture Systems for Hepatitis C Virus

Steinmann

Pietschmann

2013

Current Topics in Microbiology and Immunology

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Due to the obligatory intracellular life style of viruses, cell culture systems for efficient viral propagation are crucial to obtain a detailed understanding of the virus host cell interaction. For hepatitis C virus (HCV) the development of permissive and authentic culture models has been and continues to be a challenging task. The first ef forts to culture HCV had limited success and range back to before the virus was molecularly cloned in 1989. Since then several major breakthroughs have gradually overcome limitations in culturing the virus and sequentially permitted analysis of viral RNA replication, cell entry and ultimately the complete replication cycle in cultured cells in 2005. Until today, basic and applied HCV research greatly benefit from these tremendous efforts which spurred multiple com plementary cell based model systems for distinct steps of the HCV replication cycle. When used in combination they now permit deep insights into the fascinating biology of HCV and its interplay with the host cell. In fact drug development has been much facilitated and our understanding of the molecular determinants of HCV replication has grown in parallel to these advances. Building on this ground work and further refining our cellular models to better mimic the ar chitecture, polarization and differentiation of natural hepatocytes should reveal novel unique aspects of HCV replication. Ultimately, models to culture primary HCV isolates across all genotypes may teach us important new lessons about viral functional adaptations that have evolved in exchange with its human host and that may ex plain the variable natural course of hepatitis C.

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“…Additional relevant factors enhancing viral production may be ApoB (27), DGAT1 (13), or microsomal triglyceride transfer protein (MTP) (12,14). Notably, ApoE has recently been demonstrated to be essential for virus production; ApoE-deficient mouse hepatocytes with trans expression of HCV RNA and proteins along with ApoE are able to produce high levels of infectious virions (23).…”

mentioning

confidence: 99%

Reconstitution of the Entire Hepatitis C Virus Life Cycle in Nonhepatic Cells

Costa

Turek

Felmlee

et al. 2012

J Virol

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e Hepatitis C virus (HCV) is a human hepatotropic virus, but the relevant host factors restricting HCV infection to hepatocytes are only partially understood. We demonstrate that exogenous expression of defined host factors reconstituted the entire HCV life cycle in human nonhepatic 293T cells. This study shows robust HCV entry, RNA replication, and production of infectious virus in human nonhepatic cells and highlights key host factors required for liver tropism of HCV.

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Mouse Hepatic Cells Support Assembly of Infectious Hepatitis C Virus Particles

Cited by 100 publications

References 37 publications

Characterization of Hepatitis C Virus Particle Subpopulations Reveals Multiple Usage of the Scavenger Receptor BI for Entry Steps

Characterization of Hepatitis C Virus Particle Subpopulations Reveals Multiple Usage of the Scavenger Receptor BI for Entry Steps

Cell Culture Systems for Hepatitis C Virus

Reconstitution of the Entire Hepatitis C Virus Life Cycle in Nonhepatic Cells

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