Mouse gastric mucin: cloning and chromosomal localization

Shekels, Laurie L.; Lyftogt, Carolyn; Kieliszewski, Marcia J.; Filie, Jane D.; Kozak, Christine A.; Ho, Samuel B.

doi:10.1042/bj3110775

Cited by 72 publications

(50 citation statements)

References 55 publications

(47 reference statements)

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“…Muc2, a large secreted mucin, is a goblet cell product that is found mainly in the small intestine and colon (17). Muc3 is another intestinal mucin that has been cloned partially by two different groups of investigators (18,19). Probes for M2 and for rMuc176 both hybridize to a 9-kb RNA from small intestine and colon.…”

Section: Methodsmentioning

confidence: 99%

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Cystic fibrosis mice lacking Muc1 have reduced amounts of intestinal mucus.

Parmley¹,

Gendler²

1998

J. Clin. Invest.

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Normally a thin layer of mucus covers the surface of the gastrointestinal tract protecting the epithelial cells from their environment. In cystic fibrosis (CF), mucus accumulation is abnormally high, resulting in severe intestinal obstruction. The major structural components of mucus are large mucin glycoproteins. We determined specific mucin RNA and protein expression in the gastrointestinal tract of inbred CF transmembrane conductance regulator (CFTR) knockout (

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Section: Methodsmentioning

confidence: 99%

“…In the small intestine and colon, Muc1 is produced at very low levels by enterocytes. Muc2 and Muc5ac are secreted glycoproteins, whereas Muc3 has been described recently as a transmembrane mucin (16)(17)(18)(19). In addition to numerous tandem repeats, these mucins have cysteinerich domains that are responsible for self-dimerization.…”

Section: Introductionmentioning

confidence: 99%

Cystic fibrosis mice lacking Muc1 have reduced amounts of intestinal mucus.

Parmley¹,

Gendler²

1998

J. Clin. Invest.

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“…Rabbit anti-murine gob-5 and chicken anti-murine muc5ac were generated as previously described (41,42). Staining for gob-5 was done as previously described.…”

Section: Immunohistochemistrymentioning

confidence: 99%

CXCR2 Regulates Respiratory Syncytial Virus-Induced Airway Hyperreactivity and Mucus Overproduction

Miller

Strieter

Gruber³

et al. 2003

The Journal of Immunology

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Severe inflammation and mucus overproduction are partially responsible for respiratory syncytial virus (RSV)-induced disease in infants. Using a murine model, we characterized the virally induced chemokine receptors responsible for mediating the pathophysiological response to RSV infection, we found that CXCR2 mRNA was induced at 4 days after RSV infection. Immunohistochemical staining demonstrated that CXCR2 protein was expressed on alveolar macrophages. Immunoneutralization of CXCR2 resulted in decreased airway hyperreactivity relative to the RSV-infected controls. In addition, there was decreased mucus in the bronchoalveolar lavage fluid, decreased periodic-acid Schiff staining, and significantly less mucus-associated gob-5 mRNA and protein in anti-CXCR2-treated mice. The effects of anti-CXCR2 treatment were not a result of differences in viral clearance or neutrophil influx, as these parameters were comparable in both groups of animals. To confirm our immunoneutralization studies, we performed experiments in CXCR2−/− mice. Results in CXCR2−/− mice recapitulated results from our immunoneutralization studies. However, CXCR2−/− mice also showed a statistically significant decrease in muc5ac, relative to RSV-infected wild-type animals. Thus, CXCR2 may be a relevant target in the pathogenesis of RSV bronchiolitis, since it contributes to mucus production and airway hyperreactivity in our model of RSV infection.

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“…Gastric mucin and cathepsin E are produced by pit cells (Shekels et al, 1995;Fukamachi et al, 1994), and gastric H + ,K + -ATPase and pepsinogen are expressed in parietal (Shull and Lingrel, 1986;Smolka et al, 1983) and zymogenic cells (Fukamachi et al, 1994), respectively. The cells were tested for the mucosal cell-specific mRNA expression using a RT-PCR.…”

Section: Expression Of Cell Specific Mrnas and The Effects Of Temperamentioning

confidence: 99%