Mouse gamete interactions: The zona pellucida is the site of the acrosome reaction leading to fertilization in vitro

Florman, Harvey M.; Storey, Bayard T.

doi:10.1016/0012-1606(82)90015-x

Cited by 295 publications

(151 citation statements)

References 36 publications

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“…Sperm were recovered from the cauda epididymis, diluted to a concentration of 10 6 per milliliter in Whitten's medium supplemented with NaHCO 3 (20 mM) and BSA (10 mg/ml) and incubated (37°C in air) for up to 2 h. This medium supports sperm capacitation in vitro (54). Fertilization assays were performed as described (55) to confirm that sperm from both wild-type and homozygous mutant mice capacitate in vitro. However, this assay lacks the time resolution necessary to detect differences in capacitation time between sperm from these genotypes.…”

Section: Methodsmentioning

confidence: 99%

A polycystin-1 controls postcopulatory reproductive selection in mice

Sutton

Jungnickel

Florman

2008

Proc. Natl. Acad. Sci. U.S.A.

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Pkdrej, a member of the polycystin-1 gene family, is expressed only in the male germ line. Male mice that are homozygous for a targeted mutation in the Pkdrej allele (Pkdrej tm/tm ) are fertile in unrestricted mating trials, but exhibit lower reproductive success when competing with wild-type males in sequential mating trials and in artificial insemination of mixed-sperm populations. Following mating, sperm from Pkdrej tm/tm mice require >2 h longer than those of wild-type males to be detected within the egg/cumulus complex in the oviduct. Sperm from mice of both genotypes are able to capacitate in vitro. However, one of the component processes of capacitation, the ability to undergo a zona pellucidaevoked acrosome reaction, develops more slowly in sperm from Pkdrej tm/tm animals than in sperm from wild-type males. In contrast, a second component process of capacitation, the transition to hyperactivated flagellar motility, develops with a similar time course in both genotypes. These two behavioral consequences of capacitation, exocytotic competence and altered motility, are therefore differentially regulated. These data suggest that Pkdrej controls the timing of fertilization in vivo through effects on sperm transport and exocytotic competence and is a factor in postcopulatory sexual selection.capacitation ͉ evolution ͉ fertilization ͉ polycystin ͉ sexual selection

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Section: Methodsmentioning

confidence: 99%

A polycystin-1 controls postcopulatory reproductive selection in mice

Sutton

Jungnickel

Florman

2008

Proc. Natl. Acad. Sci. U.S.A.

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“…After the acrosomal contents are released, a second kind of interaction called "secondary binding" of the sperm to the ZP is mediated by proteins on the inner acrosomal membrane. The concept that the OAM and PM are intact when the sperm encounters the ZP arose over 25 years ago from studies in the mouse (Florman and Storey, 1982. However, there is evidence that guinea pig and human sperm in the process of undergoing acrosomal exocytosis may be competent to bind to the ZP (Huang et al, 1981.…”

Section: Acrosome Transitions and Differential Release Of Acrosomal Pmentioning

confidence: 99%

The role of the acrosomal matrix in fertilization

Buffone

Foster²,

Gerton³

2008

Int. J. Dev. Biol.

109

107

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Mammalian sperm must have properly formed acrosomes to be fully functional in the process of binding and penetrating the zona pellucida (ZP), the extracellular matrix surrounding the egg. There is much evidence to raise doubts about the old "bag of enzymes" paradigm of acrosomal function, although this is the model that seems to prevail. We concur with other scientists that acrosomal exocytosis is not an all or none event where the acrosome is either "intact" or "reacted". As determined by transmission electron microscopy of human sperm undergoing acrosomal exocytosis, six stages can be identified, with the intermediate ones involving loss of acrosomal matrix material. In the mouse, there is a temporal relationship among four stages of acrosomal exocytosis. Numerous evidences suggest a more complex role for the acrosome in fertilization in which the acrosomal matrix is a scaffold for sperm-ZP interactions that self-regulates by a controlled disassembly mechanism.

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“…Mammalian spermatozoa undergo exocytosis of the acrosomal granule (the 'acrosome reaction') in response to oocytederived stimuli [1][2][3]; exocytosis can also take place after treatment with the ionophore A23187 and Ca 2+ [4,5]. Treatment with these agonists, including A23187/Ca 2+, results in a rapid generation of diacylglycerol (DAG) due to the hydrolysis of polyphosphoinositides by phosphoinositidase C [3,[6][7][8] and hydrolysis of phosphatidylcholine by a specific phospholipase C [3,8,9].…”

Section: Introductionmentioning

confidence: 99%

rab3‐Peptide stimulates exocytosis of the ram sperm acrosome via interaction with cyclic AMP and phospholipase A₂ metabolites

Garde

Roldán

1996

FEBS Letters

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Aerosomal exocytosis triggered with A23187/Ca 2+ was enhanced by rab3AL, a synthetic peptide corresponding to the effector domain of the small GTP-binding protein rab3. Exocytosis was further enhanced when spermatozoa were also exposed to dihutyryl-cAMP, but was prevented when H-89, a protein kinase A (PKA) inhibitor, was included. The action of rab3AL was not on, or upstream of, phospholipase A2 (PLA2). Inhibition of exocytosis by the PLA2 inhibitor aristolochic acid was overcome by rab3AL when it was included together with lysophosphatidylcholine; this effect was prevented by H-89. These results suggest a functional coupling between rab3 protein, metabolites generated by PLA2, and cAMP-activated PKA, in the final steps leading to membrane fusion during acrosomal exocytosis.Key words: Exocytosis; rab3; Phospholipase Az; Lysophosphatidylcholine; cAMP; Spermatozoa that regulate docking and fusion of secretory granules with the plasma membrane [16][17][18][19].Synthetic peptides corresponding to a putative effector domain of rab3 proteins have been shown to stimulate exocytosis in permeabilized [20 23] and intact cells [24], and membrane fusion in a cell-free assay system [25]. We have therefore used a sperm model system, where cells are stimulated to undergo exocytosis with A23187/Ca 2+, to explore whether rab3 might be involved in the final steps of membrane fusion during acrosomal exocytosis. We present, for the first time, evidence suggesting that rab3 protein, together with PLA2-derived metabolites and the cAMP/PKA signalling pathway, play an important role during the final steps of acrosomal exocytosis in mammalian spermatozoa. Materials and methods

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Mouse gamete interactions: The zona pellucida is the site of the acrosome reaction leading to fertilization in vitro

Cited by 295 publications

References 36 publications

A polycystin-1 controls postcopulatory reproductive selection in mice

A polycystin-1 controls postcopulatory reproductive selection in mice

The role of the acrosomal matrix in fertilization

rab3‐Peptide stimulates exocytosis of the ram sperm acrosome via interaction with cyclic AMP and phospholipase A₂ metabolites

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Mouse gamete interactions: The zona pellucida is the site of the acrosome reaction leading to fertilization in vitro

Cited by 295 publications

References 36 publications

A polycystin-1 controls postcopulatory reproductive selection in mice

A polycystin-1 controls postcopulatory reproductive selection in mice

The role of the acrosomal matrix in fertilization

rab3‐Peptide stimulates exocytosis of the ram sperm acrosome via interaction with cyclic AMP and phospholipase A2 metabolites

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Product

Resources

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rab3‐Peptide stimulates exocytosis of the ram sperm acrosome via interaction with cyclic AMP and phospholipase A₂ metabolites