Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures

Sieber‐Blum, Maya; Hu, Yaofei

doi:10.3791/772

Cited by 11 publications

(7 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Adult neural crest-derived cells have been shown to retain stem cell properties [ 33 ]. Studies have consistently demonstrated that such neural crest stem cell (NCSC) populations often mimic transcription expression profiles of both embryonic stem cells and early neural crest cells [ 34 - 36 ].…”

Section: Introductionmentioning

confidence: 99%

Metastatic neuroblastoma cancer stem cells exhibit flexible plasticity and adaptive stemness signaling

et al. 2015

View full text Add to dashboard Cite

IntroductionHigh-risk neuroblastoma (HR-NB) presenting with hematogenous metastasis is one of the most difficult cancers to cure. Patient survival is poor. Aggressive tumors contain populations of rapidly proliferating clonogens that exhibit stem cell properties, cancer stem cells (CSCs). Conceptually, CSCs that evade intensive multimodal therapy dictate tumor progression, relapse/recurrence, and poor clinical outcomes. Herein, we investigated the plasticity and stem-cell related molecular response of aggressive metastatic neuroblastoma cells that fit the CSC model.MethodsWell-characterized clones of metastatic site-derived aggressive cells (MSDACs) from a manifold of metastatic tumors of clinically translatable HR-NB were characterized for their CSC fit by examining epithelial-to-mesenchymal transition (EMT) (E-cadherin, N-Cadherin), survival (NFκB P65, p50, IκB and pIκB) and drug resistance (ABCG2) by immunoblotting; pluripotency maintenance (Nanog, SOX2) by immunofluorescence; and EMT and stemness related transcription of 93 genes by QPCR profiling. Plasticity of MSDACs under sequential alternation of culture conditions with serum and serum-free stem-cell conditions was assessed by clonal expansion (BrdU incorporation), tumorosphere formation (anchorage independent growth), EMT and stemness related transcriptome (QPCR profiling) and validated with MYC, SOX2, EGFR, NOTCH1 and CXCL2 immunoblotting.ResultsHR-NB MSDACs maintained in alternated culture conditions, serum-free stem cell medium to growth medium with serum and vice versa identified its flexible revocable plasticity characteristics. We observed signatures of stem cell-related molecular responses consistent with phenotypic conversions. Successive reintroduction to the favorable niche not only regained identical EMT, self-renewal capacity, pluripotency maintenance, and other stem cell-related signaling events, but also instigated additional events depicting aggressive adaptive plasticity.ConclusionsTogether, these results demonstrated the flexible plasticity of HR-NB MSDACs that typically fit the CSC model, and further identified the intrinsic adaptiveness of the successive phenotype switching that clarifies the heterogeneity of HR-NB. Moreover, the continuous ongoing acquisition of stem cell-related molecular rearrangements may hold the key to the switch from favorable disease to HR-NB.Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-015-0002-8) contains supplementary material, which is available to authorized users.

show abstract

Section: Introductionmentioning

confidence: 99%

Metastatic neuroblastoma cancer stem cells exhibit flexible plasticity and adaptive stemness signaling

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Whisker follicle stem cell culture, substratum coating and immunocytochemistry Whisker follicle stem cells were cultured using an adaptation of published methods (Sieber-Blum and Hu, 2008). In brief, the dissected whisker fragment containing the stem cells was placed onto a plastic dish or coverslip coated previously with extracellular matrix (see below) with a small amount of medium and allowed to adhere for 1-2 hours.…”

Section: Histology and Immunohistochemistrymentioning

confidence: 99%

“…It has been proposed that the CD34-positive stem cells migrate along the glassy membrane of the outer root sheath to the dermal papilla, where they contribute to the development of the whisker shaft (Amoh et al, 2010). These cells can be isolated and studied in culture by explanting the whisker bulge and associated trabeculae (Sieber-Blum et al, 2004;Sieber-Blum and Hu, 2008), and have the potential to differentiate into neurons and nonneuronal cells in vitro (Sieber-Blum et al, 2004;Wong et al, 2006;Amoh et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Tenascin-C and tenascin-W in whisker follicle stem cell niches: possible roles in regulating stem cell proliferation and migration

Tucker

Ferralli

Schittny

et al. 2013

Journal of Cell Science

View full text Add to dashboard Cite

SummaryThe whisker follicle has CD34-positive stem cells that migrate from their niche near the bulge along the glassy membrane to the whisker bulb, where they participate in the formation of the whisker shaft. Using immunohistochemistry, we found the glycoprotein tenascin-C in the fibrous capsule of mouse whisker follicles, along the glassy membrane and in the trabecular region surrounding keratin-15-negative, CD34-positive stem cells. The related glycoprotein tenascin-W is found in the CD34-positive stem cell niche, in nearby trabeculae and along the glassy membrane. Tenascin-W is also found in the neural stem cell niche of nearby hair follicles. The formation of stress fibers and focal adhesion complexes in CD34-positive whisker-derived stem cells cultured on fibronectin was inhibited by both tenascin-C and tenascin-W, which is consistent with a role for these glycoproteins in promoting the migration of these cells from the niche to the whisker bulb. Tenascin-C, but not tenascin-W, increased the proliferation of whisker follicle stem cells in vitro. Thus, the CD34-positive whisker follicle stem cell niche contains both tenascin-C and tenascin-W, and these glycoproteins might play a role in directing the migration and proliferation of these stem cells.

show abstract

“…In the adult organism, progenitor cells of the neural crest are found in many types of tissues, including skin [ 10 , 11 ], dorsal root ganglia [ 12 ], adrenal medulla [ 13 ], bone marrow [ 14 ], and a number of other tissues [ 15 ]. It has been shown that adult neural crest-derived cells retain the properties of stem cells [ 16 ]. Several studies have demonstrated that such neural crest stem cell populations often mimic the transcriptional expression profiles of both embryonic stem cells and neural crest progenitor cells [ 17 ].…”

Section: Origin Of Neuroblastomamentioning

confidence: 99%

Neuroblastoma Origin and Therapeutic Targets for Immunotherapy

Холоденко

Kalinovsky

Doronin

et al. 2018

Journal of Immunology Research

127

106

View full text Add to dashboard Cite

Neuroblastoma is a pediatric solid cancer of heterogeneous clinical behavior. The unique features of this type of cancer frequently hamper the process of determining clinical presentation and predicting therapy effectiveness. The tumor can spontaneously regress without treatment or actively develop and give rise to metastases despite aggressive multimodal therapy. In recent years, immunotherapy has become one of the most promising approaches to the treatment of neuroblastoma. Still, only one drug for targeted immunotherapy of neuroblastoma, chimeric monoclonal GD2-specific antibodies, is used in the clinic today, and its application has significant limitations. In this regard, the development of effective and safe GD2-targeted immunotherapies and analysis of other potential molecular targets for the treatment of neuroblastoma represents an important and topical task. The review summarizes biological characteristics of the origin and development of neuroblastoma and outlines molecular markers of neuroblastoma and modern immunotherapy approaches directed towards these markers.

show abstract

Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures

Cited by 11 publications

References 4 publications

Metastatic neuroblastoma cancer stem cells exhibit flexible plasticity and adaptive stemness signaling

Metastatic neuroblastoma cancer stem cells exhibit flexible plasticity and adaptive stemness signaling

Tenascin-C and tenascin-W in whisker follicle stem cell niches: possible roles in regulating stem cell proliferation and migration

Neuroblastoma Origin and Therapeutic Targets for Immunotherapy

Contact Info

Product

Resources

About