Mouse embryo cryopreservation utilizing a novel high-capacity vitrification spatula

Tsang, Wai Hung; Chow, King Lau

doi:10.2144/000113125

Cited by 20 publications

(14 citation statements)

References 20 publications

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“…The surface techniques ( Fig. 1) include EM grid (Steponkus et al 1990, Martino et al 1996, minimum drop size (MDS; Arav 1992, Arav & Zeron 1997, Cryotop (Hamawaki et al 1999, Kuwayama & Kato 2000, Cryoloop (Lane et al 1999a(Lane et al , 1999b, Hemi-straw (Vanderzwalmen et al 2000), solid surface (Dinnyes et al 2000), nylon mesh (Matsumoto et al 2001), Cryoleaf (Chian et al 2005), direct cover vitrification (Chen et al 2006), fiber plug (Muthukumar et al 2008), vitrification spatula (Tsang & Chow 2009), Cryo-E (Petyim et al 2009), plastic blade (Sugiyama et al 2010), and Vitri-Inga (Almodin et al 2010). To the tubing techniques (Fig.…”

Section: Germplasm Cryopreservationmentioning

confidence: 99%

Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification

2011

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Preservation of female genetics is currently done primarily by means of oocyte and embryo cryopreservation. The field has seen much progress during its four-decade history, progress driven predominantly by research in humans, cows, and mice. Two basic cryopreservation techniques rule the field -controlled-rate freezing, the first to be developed, and vitrification, which, in recent years, has gained a foothold. While much progress has been achieved in human medicine, the cattle industry, and in laboratory animals, this is far from being the case for most other mammals and even less so for other vertebrates. The major strides and obstacles in human and other vertebrate oocyte and embryo cryopreservation will be reviewed here.

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Section: Germplasm Cryopreservationmentioning

confidence: 99%

Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification

2011

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“…We have conducted a series of experiments with ovine embryos to improve survival rate after cryopreservation by using vitrification with minimum volume methods (Cryotop and Spatula MVD). These two vitrification methods have been previously reported for human (Kuwayama, 2007) and mice embryos (Tsang and Chow, 2009), respectively. In a recent study, we found that ovine IVP embryos vitrified with Cryotop and Spatula MVD showed acceptable in vitro survival and development rate (dos Santos Neto et al, 2015).…”

Section: Are We Ready To Skip the Fresh Embryo Transfer?mentioning

confidence: 99%

Advances and limitations of in vitro embryo production in sheep and goats

Menchaca¹,

Barrera²,

Santos³

et al. 2016

Anim Reprod

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This review summarizes the latest advances and main limitations for the implementation of in vitro embryo production programs in sheep and goats. We describe the laparoscopic assisted technique for oocyte retrieval and propose new insights for follicular manipulation to improve oocyte quality. Further description of the routine conducted in our laboratory for the in vitro process of oocyte maturation, fertilization and embryo culture is presented, with emphasis in the main issues for the success of the technique. Protocols for fixed time embryo transfer (FTET) are proposed and the optimal number of in vitro produced (IVP) embryos to be transferred per female is discussed. In addition, we present pregnancy outcomes and birth rates recently obtained with FTET with IVP embryos cryopreserved by vitrification with new minimum volume methods. In summary, due to important refinements for in vitro embryo production in sheep and goats achieved in the recent years, this technology is now available for its implementation in commercial programs for genetic improvement, for the production of genetically engineered sheep and goats, and for basic research in reproduction.

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“…The vitrification spatula (VS; Tsang and Chow, 2009) and plastic blade (Sugiyama et al, 2010) are similar to the cryoloop (Lane et al, 1999) in their overall topology. All of them have their sample docking domain attached to the ceiling of the cap of cryotube to facilitate tight and easy sealing.…”

Section: Tip-type Carriersmentioning

confidence: 99%

Cryopreservation of mammalian embryos: Advancement of putting life on hold

Tsang

Chow

2010

Birth Defects Research Pt C

Self Cite

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Rodent transgenesis and human-assisted reproductive programs involve multistep handling of preimplantation embryos. The efficacy of production and quality of results from conventionally scheduled programs are limited by temporal constraints other than the quality and quantities of embryos per se. The emergence of vitrification, a water ice-free cryopreservation technique, as a reliable way to arrest further growth of preimplantation embryos, provides an option to eliminate the time constraint. In this article, current and potential applications of cryopreservation to facilitate laboratory animal experiments, colony management, and human-assisted reproductive programs are reviewed. Carrier devices developed for vitrification in the last two decades are compared with an emphasis on their physical properties that infer cooling rate of samples and sterility assurance. Biological impacts of improved cryopreservation on preimplantation embryos are also discussed.

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Mouse embryo cryopreservation utilizing a novel high-capacity vitrification spatula

Cited by 20 publications

References 20 publications

Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification

Current progress in oocyte and embryo cryopreservation by slow freezing and vitrification

Advances and limitations of in vitro embryo production in sheep and goats

Cryopreservation of mammalian embryos: Advancement of putting life on hold

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