Mouse Crossveinless-2 is the vertebrate homolog of a Drosophila extracellular regulator of BMP signaling

Coffinier, Catherine; Ketpura, Nan I.; Tran, Uyen; Geissert, Douglas; Robertis, E. M. De

doi:10.1016/s0925-4773(03)00113-8

Cited by 44 publications

(46 citation statements)

References 19 publications

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“…These weak DV patterning defects can be explained by the presence of other anti-BMP molecules acting together with Chordin. Examples include Noggin, Follistatin, Sizzled, and Crossveinless-2 (Smith and Harland, 1992;Hemmati-Brivanlou et al, 1994;Bachiller et al, 2000;Yabe et al, 2003;Martyn and Schulte-Merker, 2003;Coffinier et al, 2002;Dal-Pra et al, 2006;Rentzsch et al, 2006). Consistent with this idea, triple knockdown of noggin, follistatin, and chordin in amphibian embryo, indeed, resulted in a drastic deletion of dorsal structures (Khokha et al, 2005), which is never obtained by a single or double depletion of these genes.…”

Section: Chordin Ut006 Shows Various Degree Of Ventralized Phenotypementioning

confidence: 78%

Phenotypic analysis of a novel chordin mutant in medaka

Takashima

Shimada

Kobayashi

et al. 2007

Developmental Dynamics

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We have isolated and characterized a ventralized mutant in medaka (the Japanese killifish; Oryzias latipes), which turned out to have a mutation in the chordin gene. The mutant exhibits ventralization of the body axis, malformation of axial bones, over-bifurcation of yolk sac blood vessels, and laterality defects in internal organs. The mutant exhibits variability of phenotypes, depending on the culture temperature, from embryos with a slightly ventralized phenotype to those without any head and trunk structures. Taking

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Section: Chordin Ut006 Shows Various Degree Of Ventralized Phenotypementioning

confidence: 78%

Phenotypic analysis of a novel chordin mutant in medaka

Takashima

Shimada

Kobayashi

et al. 2007

Developmental Dynamics

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“…and M.B.O., unpublished). Vertebrate homologs of Cv2 can bind Bmps, and act variously as agonists or antagonists of Bmp signaling in different assays (Kamimura et al, 2004;Coffinier et al, 2002;Binnerts et al, 2004). It is not clear by what mechanism Cv2 promotes Bmp signaling during PCV formation.…”

Section: Discussionmentioning

confidence: 99%

Matching catalytic activity to developmental function: Tolloid-related processes Sog in order to help specify the posterior crossvein in theDrosophilawing

et al. 2005

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The Drosophila tolloid (tld) and tolloid related(tlr) gene products belong to a family of developmentally important proteases that includes Bone Morphogenetic Protein 1 (Bmp1). Tld is required early in Drosophila development for proper patterning of dorsal embryonic structures, whereas Tlr is required later during larval and pupal stages of development. The major function of Tld is to augment the activity of Decapentaplegic (Dpp) and Screw (Scw), two members of the Bmp subgroup of the Tgfβ superfamily, by cleaving the Bmp inhibitor Short gastrulation (Sog). In this study, we provide evidence that Tlr also contributes to Sog processing. Tlr cleaves Sog in vitro in a Bmp-dependent manner at the same three major sites as does Tld. However, Tlr shows different site selection preferences and cleaves Sog with slower kinetics. To test whether these differences are important in vivo, we investigated the role of Tlr and Tld during development of the posterior crossvein (PCV) in the pupal wing. We show that tlr mutants lack the PCV as a result of too little Bmp signaling. This is probably caused by excess Sog activity, as the phenotype can be suppressed by lowering Sog levels. However, Tld cannot substitute for Tlr in the PCV; in fact, misexpressed Tld can cause loss of the PCV. Reducing levels of Sog can also cause loss of the PCV, indicating that Sog has not only an inhibitory but also a positive effect on signaling in the PCV. We propose that the specific catalytic properties of Tlr and Tld have evolved to achieve the proper balance between the inhibitory and positive activities of Sog in the PCV and early embryo, respectively. We further suggest that, as in the embryo, the positive effect of Sog upon Bmp signaling probably stems from its role in a ligand transport process.

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“…Previous studies have reported Cv2 expression in embryonic and adult mouse tissues as well as in ES cells (Coffinier et al, 2002;Moser et al, 2003).…”

Section: Temporal and Spatial Expression Of Cv2 During Embryogenesismentioning

confidence: 97%

“…At E12.5, the Cv2 -/-mice exhibit no remarkable gross defects and are found in a Mendelian ratio (see Table S1 in the supplementary material; M.I. and Y.S., unpublished), even though Cv2 is expressed in the rostral mesoderm and the caudal primitive streak during gastrulation (Coffinier et al, 2002).…”

Section: The Function Of Cv2 Is Not Essential For All Bmpdependent Emmentioning

confidence: 99%

Essential pro-Bmp roles of crossveinless 2 in mouse organogenesis

et al. 2006

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We here report essential roles of the Bmp-binding protein crossveinless 2(Cv2; Bmper) in mouse organogenesis. In the null Cv2 mutant mouse,gastrulation occurs normally, but a number of defects are found in Cv2-expressing tissues such as the skeleton. Cartilage differentiation by Bmp4 treatment is reduced in cultured Cv2-/- fibroblasts. Moreover, the defects in the vertebral column and eyes of the Cv2-/- mouse are substantially enhanced by deleting one copy of the Bmp4 gene, suggesting a pro-Bmp role of Cv2 in the development of these organs. In addition, the Cv2-/- mutant exhibits substantial defects in Bmp-dependent processes of internal organ formation, such as nephron generation in the kidney. This kidney hypoplasia is synergistically enhanced by the additional deletion of Kcp (Crim2) which encodes a pro-Bmp protein structurally related to Cv2. This study demonstrates essential pro-Bmp functions of Cv2 for locally restricted signal enhancement in multiple aspects of mammalian organogenesis.

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Mouse Crossveinless-2 is the vertebrate homolog of a Drosophila extracellular regulator of BMP signaling

Cited by 44 publications

References 19 publications

Phenotypic analysis of a novel chordin mutant in medaka

Phenotypic analysis of a novel chordin mutant in medaka

Matching catalytic activity to developmental function: Tolloid-related processes Sog in order to help specify the posterior crossvein in theDrosophilawing

Essential pro-Bmp roles of crossveinless 2 in mouse organogenesis

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