Mouse and Human Mesoangioblasts: Isolation and Characterization from Adult Skeletal Muscles

Quattrocelli, Mattia; Palazzolo, Giacomo; Perini, Ilaria; Crippa, Stefania; Cassano, Marco; Sampaolesi, Maurilio

doi:10.1007/978-1-61779-343-1_4

Cited by 41 publications

(47 citation statements)

References 15 publications

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“…Pasquinelli et al (45) have shown that fresh aorta segments, harvested from heart-beating multiorgan donors, are highly suitable for obtaining MSCs confirming that the artery wall contains different populations of precursor cells (26,29,30,31,58). Furthermore, several studies have reported that a common ancestor for endothelial and mesodermal precursors, the socalled "mesoangioblast," could be isolated from the embryonic dorsal aorta (15,41,47) and also from postnatal tissue (48,59). Moreover, in 2010, Juchem et al (33) described a method for the isolation of pericyte-like cells from the intima layer.…”

mentioning

confidence: 97%

Cells derived from porcine aorta tunica media show mesenchymal stromal-like cell properties in in vitro culture

Zaniboni

Bernardini

Alessandri

et al. 2014

American Journal of Physiology-Cell Physiology

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Several studies have already described the presence of specialized niches of precursor cells in vasculature wall, and it has been shown that these populations share several features with mesenchymal stromal cells (MSCs). Considering the relevance of MSCs in the cardiovascular physiopathology and regenerative medicine, and the usefulness of the pig animal model in this field, we reported a new method for MSC-like cell isolation from pig aorta. Filling the vessel with a collagenase solution for 40 min, all endothelial cells were detached and discarded and then collagenase treatment was repeated for 4 h to digest approximately one-third of the tunica media. The ability of our method to select a population of MSC-like cells from tunica media could be ascribed in part to the elimination of contaminant cells from the intimal layer and in part to the overnight culture in the high antibiotic/antimycotic condition and to the starvation step. Aortic-derived cells show an elongated, spindle shape, fibroblast-like morphology, as reported for MSCs, stain positively for CD44, CD56, CD90, and CD105; stain negatively for CD34 and CD45; and express CD73 mRNA. Moreover, these cells show the classical mesenchymal trilineage differentiation potential. Under our in vitro culture conditions, aortic-derived cells share some phenotypical features with pericytes and are able to take part in the formation of network-like structures if cocultured with human umbilical vein endothelial cells. In conclusion, our work reports a simple and highly suitable method for obtaining large numbers of precursor MSC-like cells derived from the porcine aortic wall.

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mentioning

confidence: 97%

Cells derived from porcine aorta tunica media show mesenchymal stromal-like cell properties in in vitro culture

Zaniboni

Bernardini

Alessandri

et al. 2014

American Journal of Physiology-Cell Physiology

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“…1A). To further confirm that isolated cells expressed microvascular PCs' markers, expression of PDGFRB and ALP, two markers expressed by vesselassociated PCs [35], was verified by reverse transcriptionpolymerase chain reaction (RT-PCR) in all sk-hPCs and smhPCs (Fig. 1C).…”

Section: Resultsmentioning

confidence: 99%

Tissue-Specific Cultured Human Pericytes: Perivascular Cells from Smooth Muscle Tissue Have Restricted Mesodermal Differentiation Ability

Pierantozzi

Vezzani

Badin

et al. 2016

Stem Cells and Development

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Microvascular pericytes (PCs) are considered the adult counterpart of the embryonic mesoangioblasts, which represent a multipotent cell population that resides in the dorsal aorta of the developing embryo. Although PCs have been isolated from several adult organs and tissues, it is still controversial whether PCs from different tissues exhibit distinct differentiation potentials. To address this point, we investigated the differentiation potentials of isogenic human cultured PCs isolated from skeletal (sk-hPCs) and smooth muscle tissues (smhPCs). We found that both sk-hPCs and sm-hPCs expressed known pericytic markers and did not express endothelial, hematopoietic, and myogenic markers. Both sk-hPCs and sm-hPCs were able to differentiate into smooth muscle cells. In contrast, sk-hPCs, but not sm-hPCs, differentiated in skeletal muscle cells and osteocytes. Given the reported ability of the Notch pathway to regulate skeletal muscle and osteogenic differentiation, skhPCs and sm-hPCs were treated with N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT), a known inhibitor of Notch signaling. DAPT treatment, as assessed by histological and molecular analysis, enhanced myogenic differentiation and abolished osteogenic potential of sk-hPCs. In contrast, DAPT treatment did not affect either myogenic or osteogenic differentiation of sm-hPCs. In summary, these results indicate that, despite being isolated from the same anatomical niche, cultured PCs from skeletal muscle and smooth muscle tissues display distinct differentiation abilities.

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“…Murine dystrophic cardiac mesoangioblasts presented the aberrant propensity to differentiate into skeletal muscle fibers, which were functionally uncoupled from the surrounding myocardium and contributed to progressive exacerbation of the dilated cardiomyopathy [137,138]. The pathological myogenic switch has been imputed to miR-669a/q.…”

Section: Mirroring Skeletal Muscle Diseases and Fate Switchmentioning

confidence: 98%

The mesmiRizing complexity of microRNAs for striated muscle tissue engineering

Quattrocelli

Sampaolesi

2015

Advanced Drug Delivery Reviews

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microRNAs (miRs) are small non-protein-coding RNAs, able to post-transcriptionally regulate many genes and exert pleiotropic effects. Alteration of miR levels in tissues and in the circulation has been associated with various pathological and regenerative conditions. In this regard, tissue engineering of cardiac and skeletal muscles is a fascinating context for harnessing the complexity of miR-based circuitries and signals. In this review, we will focus on miR-driven regulation of cardiac and skeletal myogenic routes in homeostatic and challenging states. Furthermore, we will survey the intriguing perspective of exosomal and circulating miRs as novel paracrine players, potentially useful for current and future approaches of regenerative medicine for the striated muscles.

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Mouse and Human Mesoangioblasts: Isolation and Characterization from Adult Skeletal Muscles

Cited by 41 publications

References 15 publications

Cells derived from porcine aorta tunica media show mesenchymal stromal-like cell properties in in vitro culture

Cells derived from porcine aorta tunica media show mesenchymal stromal-like cell properties in in vitro culture

Tissue-Specific Cultured Human Pericytes: Perivascular Cells from Smooth Muscle Tissue Have Restricted Mesodermal Differentiation Ability

The mesmiRizing complexity of microRNAs for striated muscle tissue engineering

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