Mould Allergy

Ripe, E

doi:10.1111/j.1398-9995.1966.tb03146.x

Cited by 14 publications

(5 citation statements)

References 36 publications

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“…Allergy to house dust is well known, but not relationships: mites eating the fungal spores clearly defined (12,13,19) with the exception (easily observed in the gastrointestinal tract of the allergenic role of the house dust mites through the microscope), and moulds Dermatophagoides pteronyssinus, D. farinae and (Mucorales) with chidnolytic ability animal dander (3,15). Efforts are being decomposing the skeleton of the dead mites, made to detect the single organisms and Analyses of commercially available house components in this poorly defined dust extract (AUergologisk Laboratorium, ecosystem: house dust, consisting of textile Copenhagen) have recently been performed fibres, human dander, fungi, animal hair by means of crossed immunoelectrophoresis and dandruff, pollen, mites, baaeria and (2).…”

mentioning

confidence: 99%

Identification and Prevalence of Culturable Mesophilic Microfungi in House Dust from 100 Danish Homes

Gravesen¹

1978

Allergy

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In order to encircle possible allergen sources, fungi from house dust were cultivated and identified. Dust from vacuum cleaners was inoculated on Petri dishes containing V-8 agar with addition of penicillin and streptomycin to eliminate the bacterial flora. The number of genera identified were for the most part consistent with the genera trapped from the air. However, presumably owing to their dispersal biology it was demonstrated that members of Mucorales were much more frequently represented in the samples obtained by this method compared with gravimetric and volumetric measurements. The method is recommended as a simple way to demonstrate and identify the mould contents in house dust and as a tool for the identification of some of the real allergenic sources in house dust.

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mentioning

confidence: 99%

Identification and Prevalence of Culturable Mesophilic Microfungi in House Dust from 100 Danish Homes

Gravesen¹

1978

Allergy

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“…Ripe [19] and Nilsson and Aas [17] found that, among patients with asthma, 12 and 18°/o, respectively, were allergic to Cladosporium herbarum. In a study using RAST.…”

Section: Introductionmentioning

confidence: 99%

Crossed Radioimmunoelectrophoretic Studies of Distinct Allergens in Two Extracts of Cladosporium herbarum

Aukrust¹

1979

Int Arch Allergy Immunol

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Cladosporium herbarum was supplied from two sources and extracted identically. Antisera against the extracts were produced in rabbits and two reference patterns were established using crossed immunoelectrophoresis (CIE). Both patterns showed more than 60 precipitates but less than 50% of the detectable antigens appeared to be identical in the two extracts. The allergens were identified by means of crossed radioimmunoelectrophoresis (CRIE) using sera from 35 individuals with proven or suspected allergy to C. herbarum. Four important and 10–20 less important allergens were demonstrated. Among the allergens present, there were none reacting with all patient sera. Only 1 out of 3 rabbits immunized with a suspension of broken cells of C. herbarum showed precipitating antibodies to the statistically most important allergen, while 9 rabbits immunized with aqueous extracts of the mold did not. The composition of the two extracts with respect to allergens differed. Allergens present in one extract were not always detectable in the other. The experiments also showed how CIE/CRIE with various combinations of antigens and antisera may be combined with CRIE inhibition, radioallergosorbent test (RAST) and RAST inhibition for comparing complex allergen extracts at the molecular level.

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“…Street and Hamburger [1976] have found C. herbarum to be the dominat ing species. Ripe [1966] and Nielsson and Aas [1976) found that among patients with asthma 12 and 18n/o. respectively, were al lergic to C. herbarum.…”

Section: Introductionmentioning

confidence: 99%

Cladosporium herbarum Extract Characterized by Means of Quantitative Immunoelectrophoretic Methods with Special Attention to Immediate Type Allergy

Løwenstein¹,

Aukrust²,

Gravesen³

1977

Int Arch Allergy Immunol

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Freeze-dried extract of Cladosporium herbarum Link ex Fr. was obtained by growing, harvesting, extracting, centrifuging, dialysing and freeze-drying. Quantitative immunoelectrophoresis using rabbit antibodies revealed the extraction procedure to be reproducible and the extract to be composed of 57 antigens, none of which originated from the substrate used in the growth. The molecular weight distribution and the approximate molecular weight of some antigens of C. herbarum were obtained using gel filtration. The pI distribution and the approximate pIs of a few distinct antigens of C. herbarum were obtained by isoelectric focusing. Preliminary identification of 4 allergens from C. herbarum was performed by means of CRIE (crossed radioimmunoelectrophoresis).

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Mould Allergy

Cited by 14 publications

References 36 publications

Identification and Prevalence of Culturable Mesophilic Microfungi in House Dust from 100 Danish Homes

Identification and Prevalence of Culturable Mesophilic Microfungi in House Dust from 100 Danish Homes

Crossed Radioimmunoelectrophoretic Studies of Distinct Allergens in Two Extracts of <i>Cladosporium herbaru</i><i>m</i>

<i>Cladosporium herbarum</i> Extract Characterized by Means of Quantitative Immunoelectrophoretic Methods with Special Attention to Immediate Type Allergy

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