Motor dysfunction in Drosophila melanogaster as a biomarker for developmental neurotoxicity

Cabrita, A.; Medeiros, Alexandra M.; Pereira, Telmo; Rodrigues, Ana Margarida; Kranendonk, Michel; Mendes, César S.

doi:10.1016/j.isci.2022.104541

Cited by 9 publications

(3 citation statements)

References 112 publications

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“…7h, j). The same shift was observed in animals exposed to excessive weight bearing 27 , or during ageing 29 .…”

Section: Genipin Increases Lifespan and Motor Performance In Drosophi...supporting

confidence: 70%

“…Tripod and tetrapod indexes correspond to the fraction of frames the animal display tripod or tetrapod configurations, respectively. Since many of the measured gait parameters are speeddependent 26,29 , we analysed the data for these parameters by firstly determining the best-fit regression model for the control experiment. Subsequently the residual values for each experimental group in relation to this regression model were determined, using Rstudio 1.1.442 27 .…”

Section: Kinematic Analysismentioning

confidence: 99%

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Genipin prevents alpha-synuclein aggregation and toxicity by affecting endocytosis, metabolism and lipid storage

et al. 2023

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Parkinson’s Disease (PD) is a common neurodegenerative disorder affecting millions of people worldwide for which there are only symptomatic therapies. Small molecules able to target key pathological processes in PD have emerged as interesting options for modifying disease progression. We have previously shown that a (poly)phenol-enriched fraction (PEF) of Corema album L. leaf extract modulates central events in PD pathogenesis, namely α-synuclein (αSyn) toxicity, aggregation and clearance. PEF was now subjected to a bio-guided fractionation with the aim of identifying the critical bioactive compound. We identified genipin, an iridoid, which relieves αSyn toxicity and aggregation. Furthermore, genipin promotes metabolic alterations and modulates lipid storage and endocytosis. Importantly, genipin was able to prevent the motor deficits caused by the overexpression of αSyn in a Drosophila melanogaster model of PD. These findings widens the possibility for the exploitation of genipin for PD therapeutics.

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“…7h, j). The same shift was observed in animals exposed to excessive weight bearing 27 , or during ageing 29 .…”

Section: Genipin Increases Lifespan and Motor Performance In Drosophi...supporting

confidence: 70%

Section: Kinematic Analysismentioning

confidence: 99%

Genipin prevents alpha-synuclein aggregation and toxicity by affecting endocytosis, metabolism and lipid storage

et al. 2023

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“…Chronic dietary exposure to BMAA has been found to reduce lifespan, fertility, and motor function in Drosophila in a dosedependent manner (Zhou et al, 2009(Zhou et al, , 2010Nguyen et al, 2020;Cabrita et al, 2022). Chronic dietary exposure to BMAA has also been found to trigger neuropathology in non-human primates, including neurofibrillary tangles and sparse amyloid plaques in the brain, as well as TDP-43 +, FUS, and Ubiquitin proteinopathies in the spine (Cox et al, 2016a;Davis et al, 2020), whereas acute exposures do not produce such neuropathologies (Cox et al, 2016b).…”

Section: Figurementioning

confidence: 99%

BMAA in cycad-feeding Lepidoptera: defensive sequestration or bioaccumulation?

et al. 2023

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Many herbivorous Lepidoptera accumulate plant toxins within their own tissues as a defensive strategy. Pioneering research in this area was conducted by Miriam Rothschild and Deane Bowers, who showed that the cycad-feeding butterfly Eumaeus atala sequester the toxic plant compound cycasin and thereby deter vertebrate and invertebrate predators. The current study focuses on another cycad compound, β-methylamino-L-alanine (BMAA), that is known to accumulate in the tissues of insects and other herbivores, and which has been shown to have neurotoxic effects in humans. Chemical analyses revealed that BMAA accumulates in both immature and adult tissues of E. atala, as well as adult tissues of another cycad-feeding lepidopteran, Seirarctia echo. However, the distribution of BMAA across life stages and tissues did not conform to patterns predicted for defensive sequestration, and subsequent behavioral experiments with ants showed that these invertebrate predators were not deterred by BMAA. Our results suggest that high levels of BMAA in the tissues of cycad-feeding insects likely reflect passive bioaccumulation rather than defensive sequestration. Combined with the previous work by Rothschild and Bowers, these results provide an example in which two different plant toxins accumulate within the tissues of a single herbivore species via different mechanisms and with different implications for ecology and evolution. They thereby lay the groundwork for further investigation into the processes underlying active sequestration and non-adaptive bioaccumulation.

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Mechanosensory bristles mediate avoidance behavior by triggering sustained local motor activity in Drosophila melanogaster

Medeiros,

Hobbiss,

Borges

et al. 2024

Current Biology

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Motor dysfunction in Drosophila melanogaster as a biomarker for developmental neurotoxicity

Cited by 9 publications

References 112 publications

Genipin prevents alpha-synuclein aggregation and toxicity by affecting endocytosis, metabolism and lipid storage

Genipin prevents alpha-synuclein aggregation and toxicity by affecting endocytosis, metabolism and lipid storage

BMAA in cycad-feeding Lepidoptera: defensive sequestration or bioaccumulation?

Mechanosensory bristles mediate avoidance behavior by triggering sustained local motor activity in Drosophila melanogaster

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