Motor Behavior Mediated by Continuously Generated Dopaminergic Neurons in the Zebrafish Hypothalamus Recovers after Cell Ablation

McPherson, Adam D.; Barrios, Joshua; Luks-Morgan, Sasha J.; Manfredi, John P.; Bonkowsky, Joshua L.; Douglass, Adam D.; Dorsky, Richard I.

doi:10.1016/j.cub.2015.11.064

Cited by 54 publications

(68 citation statements)

References 28 publications

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“…In their hypothesis, TH1 expressing cells synthesize DA while TH2 expressing cells synthesize 5-HT in zebrafish. However, later studies do not support this idea (McPherson et al, 2016;Semenova, Chen, Zhao, Rauvala, & Panula, 2014). Based on our results revealing the presence of TH2 (but not necessarily TH1) in the DA 1 CFS-c cells in three vertebrate groups, it is more likely that TH2 is able to synthesize DA in this area.…”

Section: Da/5-ht Dual Phenotype Of the Hypothalamic Csf-c Cellsmentioning

(Expert classified)

Comparative analysis of monoaminergic cerebrospinal fluid‐contacting cells in Osteichthyes (bony vertebrates)

Xavier

Fontaine

Bloch

et al. 2017

J of Comparative Neurology

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Cerebrospinal fluid-contacting (CSF-c) cells containing monoamines such as dopamine (DA) and serotonin (5-HT) occur in the periventricular zones of the hypothalamic region of most vertebrates except for placental mammals. Here we compare the organization of the CSF-c cells in chicken, Xenopus, and zebrafish, by analyzing the expression of synthetic enzymes of DA and 5-HT, respectively, tyrosine hydroxylase (TH) and tryptophan hydroxylase (TPH), and draw an evolutionary scenario for this cell population. Due to the lack of TH immunoreactivity in this region, the hypothalamic CSF-c cells have been thought to take up DA from the ventricle instead of synthesizing it. We demonstrate that a second TH gene (TH2) is expressed in the CSF-c cells of all the three species, suggesting that these cells do indeed synthetize DA. Furthermore, we found that many CSF-c cells coexpress TH2 and TPH1 and contain both DA and 5-HT, a dual neurotransmitter phenotype hitherto undescribed in the brain of any vertebrate. The similarities of CSF-c cells in chicken, Xenopus, and zebrafish suggest that these characteristics are inherited from the common ancestor of the Osteichthyes. A significant difference between tetrapods and teleosts is that teleosts possess an additional CSF-c cell population around the posterior recess (PR) that has emerged in specific groups of Actinopterygii. Our comparative analysis reveals that the hypothalamus in mammals and teleosts has evolved in a divergent manner: placental mammals have lost the monoaminergic CSF-c cells, while teleosts have increased their relative number. K E Y W O R D S

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Section: Da/5-ht Dual Phenotype Of the Hypothalamic Csf-c Cellsmentioning

(Expert classified)

Comparative analysis of monoaminergic cerebrospinal fluid‐contacting cells in Osteichthyes (bony vertebrates)

Xavier

Fontaine

Bloch

et al. 2017

J of Comparative Neurology

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“…Embryos were obtained from the following zebrafish lines: Tg(her4.3: EGFP) y83 (Yeo et al, 2007), Tg(ubi:loxP-eGFP-loxP-mCherry) cz1701 (Mosimann et al, 2011), Tg(−3her4.1:ERT2-Cre-ERT2) vu298 (Boniface et al, 2009;Mosimann et al, 2011), Et(Gal4-VP16,myl7:gfp) zc1066a , Tg(UAS-E1b:NTR-mCherry) jh17 (Davison et al, 2007;Pisharath et al, 2007), Tg(7xTCF-Xla.Siam:GFP) ia4 , Tg(hsp701:dkk1-GFP) w32 (Stoick-Cooper et al, 2007), Tg(hsp70l:wnt8a-GFP) w34 (Weidinger et al, 2005), lef1 zd11 , Tg(th2: GFP-aequorin) zd201 and Tg(th2:Gal-VP16) zd202 (McPherson et al, 2016). Embryos were staged according to Kimmel et al (1995).…”

Section: Zebrafishmentioning

confidence: 99%

Hypothalamic radial glia function as self-renewing neural progenitors in the absence of Wnt/ß-catenin signaling

et al. 2015

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The vertebrate hypothalamus contains persistent radial glia that have been proposed to function as neural progenitors. In zebrafish, a high level of postembryonic hypothalamic neurogenesis has been observed, but the role of radial glia in generating these new neurons is unclear. We have used inducible Cre-mediated lineage labeling to show that a population of hypothalamic radial glia undergoes selfrenewal and generates multiple neuronal subtypes at larval stages. Whereas Wnt/β-catenin signaling has been demonstrated to promote the expansion of other stem and progenitor cell populations, we find that Wnt/β-catenin pathway activity inhibits this process in hypothalamic radial glia and is not required for their self-renewal. By contrast, Wnt/β-catenin signaling is required for the differentiation of a specific subset of radial glial neuronal progeny residing along the ventricular surface. We also show that partial genetic ablation of hypothalamic radial glia or their progeny causes a net increase in their proliferation, which is also independent of Wnt/β-catenin signaling. Hypothalamic radial glia in the zebrafish larva thus exhibit several key characteristics of a neural stem cell population, and our data support the idea that Wnt pathway function may not be homogeneous in all stem or progenitor cells.

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“…In the previous studies, we analyzed the function of Wnt signaling on adult neurogenesis in the optic tectum of zebrafish (Shitasako et al, ), and Wnt‐related genes were expressed in the central nervous system of the zebrafish embryo (Clements, Ong, & Traver, ; Duncan, Panahi, Piotrowski, & Dorsky, ; Hashimoto et al, ; Untergasser, Martowicz, Hermann, Töchterle, & Meyer, ). Moreover, other studies also showed that Wnt signaling regulates self‐renewal and differentiation of neural stem cells in the physiological and regenerative conditions in the retina, hypothalamus, and spinal cord (McPherson et al, ; Ramachandran, Zhao, & Goldman, ; Strand et al, ). These studies suggest that Wnt signaling may play an important role not only in adult neurogenesis but also in regenerative neurogenesis.…”

Section: Introductionmentioning

confidence: 99%

Wnt signaling regulates proliferation and differentiation of radial glia in regenerative processes after stab injury in the optic tectum of adult zebrafish

Shimizu

Ueda

Ohshima

2018

Glia

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Zebrafish have superior abilities to generate new neurons in the adult brain and to regenerate brain tissue after brain injury compared with mammals. There exist two types of neural stem cells (NSCs): neuroepithelial-like stem cells (NE) and radial glia (RG) in the optic tectum. We established an optic tectum stab injury model to analyze the function of NSCs in the regenerative condition and confirmed that the injury induced the proliferation of RG, but not NE and that the proliferated RG differentiated into new neurons after the injury. We then analyzed the involvement of Wnt signaling after the injury, using a Wnt reporter line in which canonical Wnt signaling activation induced GFP expression and confirmed that GFP expression was induced specifically in RG after the injury. We also analyzed the expression level of genes related to Wnt signaling, and confirmed that endogenous Wnt antagonist dkk1b expression was significantly decreased after the injury. We observed that Wnt signal inhibitor IWR1 treatment suppressed the proliferation and differentiation of RG after the injury, suggesting that up-regulation of Wnt signaling in RG after the stab injury was required for optic tectum regeneration. We also confirmed that Wnt activation by treatment with GSK3β inhibitor BIO in uninjured zebrafish induced proliferation of RG in the optic tectum. This optic tectum stab injury model is useful for the study of the molecular mechanisms of brain regeneration and analysis of the RG functions in physiological and regenerative conditions.

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Motor Behavior Mediated by Continuously Generated Dopaminergic Neurons in the Zebrafish Hypothalamus Recovers after Cell Ablation

Cited by 54 publications

References 28 publications

Comparative analysis of monoaminergic cerebrospinal fluid‐contacting cells in Osteichthyes (bony vertebrates)

Comparative analysis of monoaminergic cerebrospinal fluid‐contacting cells in Osteichthyes (bony vertebrates)

Hypothalamic radial glia function as self-renewing neural progenitors in the absence of Wnt/ß-catenin signaling

Wnt signaling regulates proliferation and differentiation of radial glia in regenerative processes after stab injury in the optic tectum of adult zebrafish

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