Motion characteristics and plasma integrity evaluation of Murrah buffalo semen

Abstract

Buffalo semen collected from Murrah bull were cryopreserved and evaluated for different motility parameter, kinematics and plasma membrane integrity. Buffalo bulls were maintained uniform standard management and nutritional practices. Semen was collected regularly twice a week semen collection schedule from four (04) Murrah bull. Collected semen was immediately transported to laboratory and evaluated for different macroscopic parameter (color, volume and thickness). Fresh semen was then diluted with saline solution and evaluated for sperm concentration, motility, sperm kinematics and morphology. Semen samples that fill all the standard were selected for freezing and diluted with Tris-egg yolk citrate diluter. Diluted semen was equilibrated, cryopreserved and finally evaluated for post thaw sperm quality. Different motility parameter (total, progressive, static and slow motility) varied significantly (p<0.01) irrespective of different freezing stages. Significantly higher progressive sperm motility and viability of buffalo spermatozoa were observed at fresh semen whereas lower progressive sperm motility and viability was found at post thaw stage. Total and progressive motility reduced by 2.5 and 2.12% following equilibration, whereas following cryopreservation, total and progressive motility reduced by 35.7 and 28.51% and static motility increases accordingly (35.4%). Significantly higher plasma membrane integrity of sperm was observed at fresh semen followed by pre freeze and post thaw semen. Following freezing, integrity of plasma membrane reduces at the rate of 10.81% and 26.7% at pre freezing and post thaw stages. Significantly higher average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), straightness (STR), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) were found for fresh semen followed by pre-freeze and post-thaw semen. Frozen buffalo semen with higher progressive motility and motion characteristics may be produced if motility losses can be reduced during freezing stage as this stage results higher motility losses. Asian Australas. J. Biosci. Biotechnol. 2022, 7(2), 75-81

Assess the quality of Murrah Buffalo (Bubalus bubalis) Bull Semen regarding seasonal and geographical variability in Nepal

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Sperm quality and fertility of Murrah and Nili-Ravi bulls compared with indigenous buffalo bulls in Bangladesh

“…Collected semen from each bull was transferred to the laboratory for evaluation and further processing. Motility, morphology, concentration and kinematics were measured using a Computer Assisted Sperm Analyzer (CASA) (Hamilton Throne, IVOS II) (Miraz et al 2022). The CASA settings for analyzing sperm motility were set as; Frame rate 60Hz, Frames acquired 30, Minimum contrast 35, Minimum cell size, 5 pixels, Cell size, 9 pixels, Cell intensity 110 pixels, Path velocity (VAP) 50 μm/s, Straightness (STR) 70%, VAP cut-off, 30 μ/s and VSL cut-off 15 μ/s.…”

Age Effects on Semen Quality and Sperm Kinematics of Red Chittagong Cattle (RCC) Bull in Bangladesh

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