Motion Analysis of Intracellular Objects: Trajectories with and without Visible Tracks

Weiss, Dieter G.; Galfe, Günther; Gulden, Josef; Seitz‐Tutter, Dieter; Langford, George M.; Strüppler, A.; Weindl, A.

doi:10.1007/978-3-642-51664-1_7

Cited by 3 publications

(2 citation statements)

References 48 publications

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“…Koles et al (14) and Weiss and co-workers (40,41) proposed a quantitative method to analyze the velocity in their attempts to classify organelle movements based on movement generation type. Their analysis procedure starts off with reconstruction of the underlying microtubule by linear regression.…”

Section: Classification Strategymentioning

confidence: 99%

Classification of organelle trajectories using region-based curve analysis

et al. 1997

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A method based on analysis of the region of movement and the functioning of the acto‐myosin cytoskeleton has been elaborated to quantify and classify patterns of organelle movement in tobacco pollen tubes. The trajectory was dilated to the region of movement, which was then reduced to give a one‐pixel‐wide skeleton, represented by a graph structure. The longest line in this skeleton was hypothesized to represent the basic track of the organelle along a single actin filament. Quantitative features were derived from the graph structure, direction of movement on the longest skeletal line, and distance between skeletal line and particle. These features corresponded to biological events like the amount of linear movement or the probability of attachment of an organelle to the actin filament. From 81 analyzed organelle trajectories, 17 had completely linear, 17 had completely non‐linear, and 47 had alternating linear and non‐linear movement. Selected features were employed for classification and ranking of the movement patterns of a representative sample of the population of organelles moving in the cell tip. The presented methods can be applied to any field where analysis and classification of particle motion are intended. Cytometry 29:136–146, 1997. © 1997 Wiley‐Liss, Inc.

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Section: Classification Strategymentioning

confidence: 99%

Classification of organelle trajectories using region-based curve analysis

et al. 1997

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“…The displacement vs time plots were quantitatively evaluated using the motion analysis and statistical software packages PARTI-MOVI and SPSS as previously described (Weiss et al, 1988(Weiss et al, , 1991.…”

Section: Motile Activity and Velocity Measurementsmentioning

confidence: 99%

Movement of axoplasmic organelles on actin filaments from skeletal muscle

Kuznetsov

Rivera

Severin

et al. 1994

Cell Motil. Cytoskeleton

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It was recently shown that, in addition to the well‐established microtubule‐dependent mechanism, fast transport of organelles in squid giant axons also occurs in the presence of actin filaments [Kuznetsov et al., 1992, Nature 356:722‐725]. The objectives of this study were to obtain direct evidence of axoplasmic organelle movement on actin filaments and to demonstrate that these organelles are able to move on skeletal muscle actin filaments. Organelles and actin filaments were visualized by video‐enhanced contrast differential interference contrast (AVEC‐DIC) microscopy and by video intensified fluorescence microscopy. Actin filaments, prepared by polymerization of monomeric actin purified from rabbit skeletal muscle, were stabilized with rhodamine‐phalloidin and adsorbed to cover slips. When axoplasm was extruded on these cover slips in the buffer containing cytochalasin B that prevents the formation of endogenous axonal actin filaments, organelles were observed to move at the fast transport rate. Also, axoplasmic organelles were observed to move on bundles of actin filaments that were of sufficient thickness to be detected directly by AVEC‐DIC microscopy. The range of average velocities of movement on the muscle actin filaments was not statistically different from that on axonal filaments. The level of motile activity (number of organelles moving/min/field) on the exogenous filaments was less than on endogenous filaments probably due to the entanglement of filaments on the cover slip surface. We also found that calmodulin (CaM) increased the level of motile activity of organelles on actin filaments. In addition, CaM stimulated the movement of elongated membranous organelles that appeared to be tubular elements of smooth endoplasmic reticulum or extensions of prelysosomes. These studies provide the first direct evidence that organelles from higher animal cells such as neurons move on biochemically defined actin filaments. © 1994 Wiley‐Liss, Inc.

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Cytoplasmic Streaming: from Flow to Track

Grolig

Pierson

2000

Actin: A Dynamic Framework for Multiple Plant Cell Functions

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Motion Analysis of Intracellular Objects: Trajectories with and without Visible Tracks

Cited by 3 publications

References 48 publications

Classification of organelle trajectories using region-based curve analysis

Classification of organelle trajectories using region-based curve analysis

Movement of axoplasmic organelles on actin filaments from skeletal muscle

Cytoplasmic Streaming: from Flow to Track

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