Motilimonas cestriensis sp. nov., isolated from an inland brine spring in Northern England

Abstract

A novel slightly halophilic Gram-stain-negative bacterial strain (MKS20T) was isolated from a brine sample collected from one of the Anderton brine springs in the Cheshire salt district, located in Northern England. Phylogenetic analysis of the 16S rRNA gene sequence revealed a close proximity to Motilimonas eburnea (98.30 %), followed by Motilimonas pumila (96.62 %), the two currently described species within the genus Motilimonas . Strain MKS20T forms white-beige-pigmented colonies and grows optimally at 28–30 °C, in 1–3 % (w/v) NaCl and at pH 7–7.5. The strain was facultatively anaerobic and showed a broader range of carbohydrate use than other species in the genus Motilimonas . Q-8 was the sole respiratory quinone and the major fatty acids (>10 %) were summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and C16 : 0. The polar lipid profile included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidyglycerol and several unidentified lipids. The G+C content of the genomic DNA was 44.2 mol%. Average nucleotide identity and DNA–DNA hybridization data were consistent with assignment to a separate species. Based on the phylogenetic and genomic-based analyses, as well as physiological and biochemical characteristics, we propose that strain MKS20T (=DSM 109936T, MCCC 1K04071T) represents a new species of the genus Motilimonas , with the name Motilimonas cestriensis sp. nov.

Eco‐phylogenetic analyses reveal divergent evolution of vitamin B12 metabolism in the marine bacterial family ‘Psychromonadaceae’

“…pumila PLHSC7‐2 (95.3% identity) (Wang et al ., 2019), along with three unidentified bacterial strains (B01, D12 and A11) isolated from the coastal marine sediment (Jinjun et al ., 2013). In line with previous results of 16S rRNA tree placements (Ling et al ., 2017; Kelbrick et al ., 2021), our Motilimonas clade further formed a cluster proximal to the Psychromonas clade (bootstrap 1.0; average identity <93%) (Fig. 1A).…”

“…Our results showed that the 16S rRNA based Maximum‐likelihood (ML) tree firmly place the strain E26 into a clade formed by the three validly published Motilimonas species (bootstrap >0.7) including M . cestriensis MKS20 (98.8% identity) (Kelbrick et al ., 2021), M . eburnea YH6 (97.0% identity) (Ling et al ., 2017) and M .…”

“…cestriensis MKS20 (PRJEB39676) and M . eburnean YH6 (PRJEB39823) (Kelbrick et al ., 2021) were respectively trimmed by Trimmomatic v0.36 and assembled using SPAdes v3.13 (Bankevich et al ., 2012).…”

“…pumila (Wang et al ., 2019) and M . cestriensis (Kelbrick et al ., 2021) that were isolated from the gut of sea cucumber and inland brine spring respectively. Phylogenetic inference by 16S rRNA tree placements had suggested that Motilimonas is closely related to Psychromonas (Ling et al ., 2017; Kelbrick et al ., 2021), indicating the phylogenetic position and taxonomic status of these two genera remain somewhat cryptic.…”

“…cestriensis (Kelbrick et al ., 2021) that were isolated from the gut of sea cucumber and inland brine spring respectively. Phylogenetic inference by 16S rRNA tree placements had suggested that Motilimonas is closely related to Psychromonas (Ling et al ., 2017; Kelbrick et al ., 2021), indicating the phylogenetic position and taxonomic status of these two genera remain somewhat cryptic. Although disparate phylogenetic conclusions may be evident from different approaches, genome‐level comparisons are likely to be more robust if only because the entirety of genetic data that are considered.…”