Mothers’ command: phenotypes changes resulting from reciprocal interploidy crosses

Peer Review #2 of "In vitro induction and identification of polyploid Neolamarckia cadamba plants by colchicine treatment (v0.1)"

“…This characteristic is in line with several Manuscript to be reviewed other induced polyploid species, such as Anthurium andraeanum (Chen et al, 2011); Lobularia maritima (Huang et al, 2015); Lycium rhuthenicum (Rao et al, 2019); Manihot esculenta (Zhou et al, 2017); Bacopa monnieri (Inthima & Sujipuli, 2019); Plumbago auriculata (Jiang et al, 2020) and Solanum lycopersicum (de Alencar et al, 2020). Detailed histological study on S. lycopersicum leaf blade revealed that thicker leaf in the induced polyploid was the result of greater thickness in the epidermis (adaxial and abaxial) and the parenchyma (spongy and palisade) layers (de Alencar et al, 2020). In the induced polyploid of Plumbago auriculata, the histological study showed that thicker spongy tissue contributed to thicker leaf (Jiang et al, 2020).…”

Cytogenetic, chromosome count optimization and automation of Neolamarckia cadamba (Rubiaceae) root tips derived from in vitro mutagenesis

“…In classical plant systematic study, chromosome count data are used to categorize plants and to identify relatedness between species in a family (Lee, 1979;Puangsomlee and Puff, 2001). In plant breeding, chromosome count is also used to detect the occurrence of polyploidy (Martin et al, 2019), double haploids (Kim et al, 2019) and sometimes interploids (de Alencar et al, 2020). Although chromosome count protocol was developed decades ago, it is still primarily conducted manually through sample preparation and microscopic observation.…”

“…However, the vast number of diverse plants in planta kingdom are varied in terms of cell size, the composition of cytoplasm, chromosome number and size, cell wall thickness and so forth. These variations render modification in steps difficult to undertake, such as the concentration of reagents, treatment durations and squashing methods to obtain an accurate result (de Alencar et al, 2020;Jiang et al, 2020). Most reagents and stains in chromosome count studies were developed many decades ago.…”

Chromosome count improvement and digitalization of Neolamarckia cadamba

“…In classical plant systematic study, chromosome count data are used to categorize plants and to identify relatedness between species in a family [1,2]. In plant breeding, chromosome count is also used to identify the production of polyploids [3], double haploids [4] and interploids [5]. Although chromosome count protocol has been developed for a long time ago, it is still primarily conducted manually through sample preparation and microscopic observation.…”

“…However, the vast number of diverse plants in planta kingdom are varied in terms of cell size, the composition of cytoplasm, chromosome size, cell wall thickness and so forth. These variations render modi cation in steps di cult to undertake, such as the concentration of reagents, treatment durations and squashing methods to obtain an accurate result [5,17]. Most reagents and stains in chromosome count study were developed many decades ago.…”