Mosses do express conventional, distantly B‐type‐related phytochromes phytochrome of <i>Physcomitrella patens</i> (Hedw.)

Kolukisaoglu, H. Üner; Braun, Birgit; Martin, William; Schneider‐Poetsch, Hansjörg A. W.

doi:10.1016/0014-5793(93)81689-w

Cited by 30 publications

(13 citation statements)

References 16 publications

(23 reference statements)

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“…In P. patens, because the red-light induction of chloroplast movement is canceled by far-red light irradiation, the photoreceptor for this response is probably phytochrome (Kadota et al, 2000). Because no phy3-type chimera protein was isolated from P. patens in our screening (data not shown), conventional phytochromes, four of which are registered in the GenBank/EBI/DDBJ database and a previous report (Kolukisaoglu et al, 1993), are likely the primary photoreceptor for red light-induced chloroplast movement of P. patens.…”

Section: Discussionmentioning

confidence: 99%

Phototropins Mediate Blue and Red Light-Induced Chloroplast Movements in Physcomitrella patens

et al. 2004

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Phototropin is the blue-light receptor that mediates phototropism, chloroplast movement, and stomatal opening in Arabidopsis. Blue and red light induce chloroplast movement in the moss Physcomitrella patens. To study the photoreceptors for chloroplast movement in P. patens, four phototropin genes (PHOTA1, PHOTA2, PHOTB1, and PHOTB2) were isolated by screening cDNA libraries. These genes were classified into two groups (PHOTA and PHOTB) on the basis of their deduced amino acid sequences. Then phototropin disruptants were generated by homologous recombination and used for analysis of chloroplast movement. Data revealed that blue light-induced chloroplast movement was mediated by phototropins in P. patens. Both photA and photB groups were able to mediate chloroplast avoidance, as has been reported for Arabidopsis phot2, although the photA group contributed more to the response. Red light-induced chloroplast movement was also significantly reduced in photA2photB1photB2 triple disruptants. Because the primary photoreceptor for red light-induced chloroplast movement in P. patens is phytochrome, phototropins may be downstream components of phytochromes in the signaling pathway. To our knowledge, this work is the first to show a function for the phototropin blue-light receptor in a response to wavelengths that it does not absorb.Blue light regulates a wide variety of photoresponses in plants, including chloroplast movement, inhibition of hypocotyl elongation, circadian timing, regulation of gene expression, and stomatal opening (Briggs and Huala, 1999;Christie and Briggs, 2001;Gyula et al., 2003). Three types of flavin-containing photoreceptors (phototropin, cryptochrome, and ZTL/FKF/ADO/LKP) have been identified as the blue-light receptors that mediate these responses Schultz et al., 2001;Briggs and Christie, 2002;Cashmore, 2003;Imaizumi et al., 2003;Lin and Shalitin, 2003;Liscum et al., 2003).The phototropin gene PHOT1, first isolated in Arabidopsis, was shown to control phototropism (Huala et al., 1997). Phototropin contains a Ser/Thr protein kinase domain at the C terminus and two light, oxygen, or voltage (LOV) domains (LOV1 and LOV2) at the N terminus (Huala et al., 1997). The LOV domains function as binding sites for the chromophore FMN Kasahara et al., 2002b) and belong to the PAS domain superfamily (Taylor and Zhulin, 1999). LOV1 and LOV2 are approximately 100 amino acids in length and are separated by a variable intervening sequence. Two phototropins, phot1 and phot2, function in Arabidopsis to control chloroplast movement and stomatal opening in addition to phototropism Kagawa et al., 2001;Kinoshita et al., 2001;Sakai et al., 2001;Briggs and Christie, 2002).Chloroplasts move to different locations in plant cells depending upon the intensity of light exposure. Under low-fluence-rate light conditions, chloroplasts spread over the cell surface perpendicular to the light direction, in order to harvest sufficient light and to maximize photosynthetic activity (Zurzycki, 1955). Under high-fluence-rate light conditio...

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Section: Discussionmentioning

confidence: 99%

Phototropins Mediate Blue and Red Light-Induced Chloroplast Movements in Physcomitrella patens

et al. 2004

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“…The advantages of P. patens as a plant model system are : (1) its relative simplicity, (2) shared characteristics with higher plants, e.g. phytohormones (Wang et al, 1980(Wang et al, , 1981aAshton et al, 1985), hormone uptake (Ashton et al, 1990) and hormone response mechanisms (Knight et al, 1995), phytochrome (Kolukisaoglu et al, 1993) and phytochrome-controlled photomorphogenesis (Cove et al, 1978 ;Jenkins & Cove 1983), (3) ease of axenic propagation under controllable conditions (Ashton & Cove, 1977 ;Knight et al, 1988) and (4) its amenability to study *Author for correspondence (fax j1 306 585 4894 ; e-mail ashton!uregina.ca). using genetic (Ashton et al, 1988), physiological and cytological (Doonan & Duckett, 1988) methods.…”

Section: mentioning

confidence: 99%

The bryophyte Physcomitrella patens replicates extrachromosomal transgenic elements

et al. 2000

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Physcomitrella patens, recently renamed Aphanoregma patens, has been transformed with the plasmid, pBI426. On selective medium approx. 30% of regenerants expressed the transformed phenotype transiently (transients). The remaining 70% (transformants) retained their transformed phenotype (GUS-positive and resistant to G418) indefinitely when subcultured repeatedly on selective medium. However, most lost this phenotype after one or two passages through nonselective medium (unstable transformants). Approximately 0n2% of transformants retained their transformed phenotype after numerous passages through nonselective medium (stable transformants). Using PCR methodology, it has been shown that loss of the transformed phenotype by unstable transformants is invariably accompanied by disappearance of the transgenic DNA. Southern blot analysis data argue strongly that unstable transformants cultured under selective conditions contain unintegrated pBI426 as circular concatenates consisting of 3-40 copies of the plasmid. Under selective conditions, it appears that replication and\or partitioning of these extrachromosomal concatemers might be growth rate-limiting. This is the first report of a transgenic, autonomously replicating extrachromosomal element in a photosynthetic plant. A single copy of pBI426 has been inserted into the moss genome in each of three stable transformants analysed.

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“…The phytochrome gene, which encodes the red͞far-red light receptor and is well characterized in flowering plants, has been cloned from mosses (4,5). Phototropic responses, which are implicated in the light-associated signal transduction network, have also been reported in mosses (6).…”

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confidence: 99%

Comparative genomics ofPhyscomitrella patensgametophytic transcriptome andArabidopsis thaliana: Implication for land plant evolution

Nishiyama

Fujita

Shin-I

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

324

266

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The mosses and flowering plants diverged >400 million years ago. The mosses have haploid-dominant life cycles, whereas the flowering plants are diploid-dominant. The common ancestors of land plants have been inferred to be haploid-dominant, suggesting that genes used in the diploid body of flowering plants were recruited from the genes used in the haploid body of the ancestors during the evolution of land plants. To assess this evolutionary hypothesis, we constructed an EST library of the moss Physcomitrella patens, and compared the moss transcriptome to the genome of Arabidopsis thaliana. We constructed full-length enriched cDNA libraries from auxin-treated, cytokinin-treated, and untreated gametophytes of P. patens, and sequenced both ends of >40,000 clones. These data, together with the mRNA sequences in the public databases, were assembled into 15,883 putative transcripts. Sequence comparisons of A. thaliana and P. patens showed that at least 66% of the A. thaliana genes had homologues in P. patens. Comparison of the P. patens putative transcripts with all known proteins, revealed 9,907 putative transcripts with high levels of similarity to vascular plant genes, and 850 putative transcripts with high levels of similarity to other organisms. The haploid transcriptome of P. patens appears to be quite similar to the A. thaliana genome, supporting the evolutionary hypothesis. Our study also revealed that a number of genes are moss specific and were lost in the flowering plant lineage.

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Mosses do express conventional, distantly B‐type‐related phytochromes phytochrome of Physcomitrella patens (Hedw.)

Cited by 30 publications

References 16 publications

Phototropins Mediate Blue and Red Light-Induced Chloroplast Movements in Physcomitrella patens

Phototropins Mediate Blue and Red Light-Induced Chloroplast Movements in Physcomitrella patens

The bryophyte Physcomitrella patens replicates extrachromosomal transgenic elements

Comparative genomics ofPhyscomitrella patensgametophytic transcriptome andArabidopsis thaliana: Implication for land plant evolution

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