Moss as a Model System for Plant Stress Responses

Cuming, Andrew C.

doi:10.1002/9783527628964.ch2

Cited by 5 publications

(4 citation statements)

References 84 publications

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“…The distance between the smartphone and the eyepiece was 2.2 cm. 19 Because Physcomitrella is a model moss for molecular, cellular, and developmental biology, 20,21 we chose Physcomitrella as the first observation target of our field-portable microscopy. In Fig.…”

Section: Methodsmentioning

confidence: 99%

Mobile-phone-based Rheinberg microscope with a light-emitting diode array

et al. 2018

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Mobile phone technology has led to implementation of portable and inexpensive microscopes. Lightemitting diode (LED) array microscopes support various multicontrast imaging by flexible illumination patterns of the LED array that can be achieved without changing the optical components of the microscope. Here, we demonstrate a mobile-phone-based LED array microscope to realize multimodal imaging with bright-field, dark-field, differential phase-contrast, and Rheinberg illuminations using as few as 37 LED bulbs. Using this microscope, we obtained high-contrast images of living cells. Furthermore, by changing the color combinations of Rheinberg illumination, we were able to obtain images of living chromatic structures with enhanced or diminished contrast. This technique is expected to be a foundation for high-contrast microscopy used in modern field studies. © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Section: Methodsmentioning

confidence: 99%

Mobile-phone-based Rheinberg microscope with a light-emitting diode array

et al. 2018

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“…ABA serves as a phytohormone not only in angiosperms but also in bryophytes with a gametophyte-dominant life cycle. ABA induces growth secession and freezing and desiccation tolerance and also the formation of vegetative propagules called brood cells in the moss Physcomitrium patens (formerly known as Physcomitrella patens ; Cuming, 2009 ). Disruption of PP2C-A in the ppabi1a/b double mutant caused constitutive desiccation tolerance with enhanced SnRK2 activity, and disruption of SnRK2 in the ppsnrk2a/b/c/d quadruple mutant abolished ABA and osmostress responses, indicating the conservation of core signaling mechanisms in the moss ( Komatsu et al, 2013 ; Shinozawa et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Differential regulations of abscisic acid-induced desiccation tolerance and vegetative dormancy by group B3 Raf kinases in liverworts

et al. 2022

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Phytohormone abscisic acid (ABA) plays a key role in stomata closure, osmostress acclimation, and vegetative and embryonic dormancy. Group B3 Raf protein kinases (B3-Rafs) serve as positive regulators of ABA and osmostress signaling in the moss Physcomitrium patens and the angiosperm Arabidopsis thaliana. While P. patens has a single B3-Raf called ARK, specific members of B3-Rafs among six paralogs regulate ABA and osmostress signaling in A. thaliana, indicating functional diversification of B3-Rafs in angiosperms. However, we found that the liverwort Marchantia polymorpha, belonging to another class of bryophytes, has three paralogs of B3-Rafs, MpARK1, MpARK2, and MpARK3, with structural variations in the regulatory domains of the polypeptides. By reporter assays of the P. patens ark line and analysis of genome-editing lines of M. polymorpha, we found that these B3-Rafs are functionally redundant in ABA response, with respect to inhibition of growth, tolerance to desiccation and expression of stress-associated transcripts, the majority of which are under the control of the PYR/PYL/RCAR-like receptor MpPYL1. Interestingly, gemmae in gemma cups were germinating only in mutant lines associated with MpARK1, indicating that dormancy in the gametophyte is controlled by a specific B3-Raf paralog. These results indicated not only conservation of the role of B3-Rafs in ABA and osmostress response in liverworts but also functional diversification of B3-Rafs, which is likely to have occurred in the early stages of land plant evolution.

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“…These plants diverged from their sister group of vascular plants ~500 mya, and they are non-vascular, gametophyte-dominant (haploid) plants with a relatively low degree of morphological and anatomical complexity, and a low capacity of self-internal regulation due to their poikilohydric nature (Vanderpoorten & Goffinet, 2009). Since they use a variety of unique metabolic pathways to deal with environmental challenges (Cuming 2009;Glime, 2017a,b), their study could provide important insights into the evolution of these responses.…”

Section: Introductionmentioning

confidence: 99%

Patterns and mechanisms of heavy metal accumulation and tolerance in two terrestrial moss species with contrasting habitat specialization

Boquete

Lang

Weidinger³

et al. 2020

Preprint

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Phenotypic variation in natural populations is crucial for rapid adjustment to challenging environmental conditions such as exposure to heavy metal pollution. Hence, the study of variation in traits related to plant response to heavy metal stress provides valuable insight into the likelihood of a population's survival. We investigate the patterns and mechanisms of intraspecific phenotypic variation for heavy metal accumulation and tolerance in bryophytes, one of the most resilient yet relatively understudied taxa. Two terrestrial mosses exposed to Cd and Cu in the laboratory, the heavy metal specialist Scopelophila cataractae and the facultative metallophyte Ceratodon purpureus, showed intraspecific differences in tolerance to these metals.Only the heavy metal specialist showed differences in accumulation which in the case of Cu could be associated to preferential relocation towards the stem to protect their main photosynthetic organs. We also report the first evidence for sexual dimorphism for Cd tolerance in C. purpureus (females being more tolerant than males). Our results provide support for high variation in the capacity of bryophytes to respond to environmental challenge despite potentially low levels of genetic variation and lack of previous exposure to stress, as well as evidence for metal-dependent, sex-specific differences in heavy metal tolerance in bryophytes. Zachary D. Atlas and Dr. David B. Lewis for providing support and infrastructure for soil analyses at USF. SEM-EDX was performed at the Core Facility Cell Imaging & Ultrastructure Research, University of Vienna -member of the Vienna Life-Science Instruments (VLSI) and we thank Dr. Irene Lichtscheidl for her support. This research has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sk lodowska-Curie grant agreement No 704141-BryOmics.

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Moss as a Model System for Plant Stress Responses

Cited by 5 publications

References 84 publications

Mobile-phone-based Rheinberg microscope with a light-emitting diode array

Mobile-phone-based Rheinberg microscope with a light-emitting diode array

Differential regulations of abscisic acid-induced desiccation tolerance and vegetative dormancy by group B3 Raf kinases in liverworts

Patterns and mechanisms of heavy metal accumulation and tolerance in two terrestrial moss species with contrasting habitat specialization

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