Mosquito-specific microRNA-1890 targets the juvenile hormone-regulated serine protease JHA15 in the female mosquito gut

Lucas, Keira J.; Zhao, Bo; Roy, Sourav; Gervaise, Amanda L.; Raikhel, Alexander S.

doi:10.1080/15476286.2015.1101525

Cited by 53 publications

(51 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies illustrated that miR-275, miR-1174, miR-8, and miR-1890 are essential for the normal progression of blood meal digestion and egg development of Ae. aegypti (29)(30)(31)(32). However, our knowledge of the role of miRNAs in posttranscriptional regulation of the FB genes affecting these tissue functions is limited.…”

Section: Significancementioning

confidence: 99%

Transcriptome-wide microRNA and target dynamics in the fat body during the gonadotrophic cycle of Aedes aegypti

Zhang

Aksoy

Girke

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The mosquito Aedes aegypti is a major vector of numerous viral diseases, because it requires a blood meal to facilitate egg development. The fat body, a counterpart of mammalian liver and adipose tissues, is the metabolic center, playing a key role in reproduction. Therefore, understanding of regulatory networks controlling its functions is critical, and the role of microRNAs (miRNAs) in the process is largely unknown. We aimed to explore miRNA expression and potential targets in the female fat body of Ae. aegypti, as well as their changes postblood meal (PBM). Small RNA library analysis revealed five unique miRNA patterns sequentially expressed at five sampled time points, likely responding to, and affecting, waves of upstream hormonal signals and gene expression in the same period. To link miRNA identities with downstream targets, transcriptome-wide mRNA 3′ UTR interaction sites were experimentally determined at 72 h posteclosion and 24 h PBM through Argonaute 1 cross-linking and immunoprecipitation followed by high-throughput sequencing. Several target sites were validated by means of in vitro luciferase assays with wild-type and mutated 3′ UTRs for six miRNA families. With established transgenic lines, consistent results were observed with spatiotemporal knockdown of miR-8 and luciferase assays. We further investigated miRNAs potentially regulating various physiological processes based on Clusters of Orthologous Groups functional categories. Hence, the present work comprehensively elucidated miRNA expression and target dynamics in the female mosquito fat body, providing a solid foundation for future functional studies of miRNA regulation during the gonadotrophic cycle.Aedes aegypti | fat body | microRNA | microRNA targets | Argonaute 1

show abstract

Section: Significancementioning

confidence: 99%

Transcriptome-wide microRNA and target dynamics in the fat body during the gonadotrophic cycle of Aedes aegypti

Zhang

Aksoy

Girke

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Along the same lines, genes encoding proteins associated with metabolism of hormones, such as the juvenile hormone and ecdysone, were down-regulated on days 4 and 6. Such hormone levels change during blood digestion [26], and relevantly control the expression of midgut serine proteases [27][28][29], which are also down-regulated upon Leishmania infection on days 4 and 6. Together, these data suggest that the sand y transcription factor Forkhead/HNF-3 as well as hormone metabolic enzymes might be key targets to control Leishmania infection early on.…”

Section: Discussionmentioning

confidence: 99%

Leishmania infection induces a limited differential gene expression in the sand fly midgut

Coutinho-Abreu

Serafim

Meneses

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Sand flies are the vectors of Leishmania parasites. To develop in the sand fly midgut, Leishmania multiplies and undergoes various stage differentiations giving rise to the infective form, the metacyclic promastigotes. To determine the changes in sand fly midgut gene expression caused by the presence of Leishmania, we performed RNA-Seq of uninfected and Leishmania infantum-infected Lutzomyia longipalpis midguts from seven different libraries corresponding to time points which cover the various Leishmania developmental stages. Results: The combined transcriptomes resulted in the de novo assembly of 13,841 sand fly midgut transcripts. Importantly, only 113 sand fly transcripts, about 1%, were differentially expressed in the presence of Leishmania parasites. Further, we observed distinct differentially expressed sand fly midgut transcripts corresponding to the presence of each of the various Leishmania stages suggesting that each parasite stage influences midgut gene expression in a specific manner. Two main patterns of sand fly gene expression modulation were noted. At early time points (days 1-4), more transcripts were down-regulated by Leishmania infection at large fold changes (> 32 fold). Among the down-regulated genes, the transcription factor Forkhead/HNF-3 and hormone degradation enzymes were differentially regulated on day 2 and appear to be the upstream regulators of nutrient transport, digestive enzymes, and peritrophic matrix proteins. Conversely, at later time points (days 6 onwards), most of the differentially expressed transcripts were up-regulated by Leishmania infection with small fold changes (< 32 fold). The molecular functions of these genes have been associated with the metabolism of lipids and detoxification of xenobiotics. Conclusion: Overall, our data suggest that the presence of Leishmania produces a limited change in the midgut transcript expression profile in sand flies. Further, Leishmania modulates sand fly gene expression early on in the developmental cycle in order to overcome the barriers imposed by the midgut, yet it behaves like a commensal at later time points where a massive number of parasites in the anterior midgut results only in modest changes in midgut gene expression.

show abstract

“…Along the same lines, genes encoding proteins associated with metabolism of hormones, such as the juvenile hormone and ecdysone, were down-regulated on days 4 and 6. Such hormone levels change during blood digestion [25], and relevantly control the expression of sand fly midgut serine proteases [26–28], which are also down-regulated upon Leishmania infection on days 4 and 6. Together, these data suggest that the sand fly transcription factor Forkhead/HNF-3 as well as hormone metabolic enzymes might be key targets to control Leishmania infection early on.…”

Section: Discussionmentioning

confidence: 99%

Leishmania infection induces a limited differential gene expression in the sand fly midgut

Coutinho-Abreu

Serafim

Meneses

et al. 2019

Preprint

View full text Add to dashboard Cite

22Background: Phlebotomine sand flies are the vectors of Leishmania worldwide. To develop in 23 the sand fly midgut, Leishmania multiplies and undergoes multiple stage differentiations leading 24 to the infective form, the metacyclic promastigotes. To gain a better understanding of the 25 influence of Leishmania infection in midgut gene expression, we performed RNA-Seq 26 comparing uninfected Lutzomyia longipalpis midguts and Leishmania infantum-infected 27 Lutzomyia longipalpis midguts at seven time points which cover the various developmental 28Leishmania stages including early time points when blood digestion is taking place and late time 29 points when the parasites are undergoing metacyclogenesis. 30Results: Out of over 13,841 transcripts assembled de novo, only 113 sand fly transcripts, about 31 1%, were differentially expressed. Further, we observed a low overlap of differentially expressed 32 sand fly transcripts across different time points suggesting a specific influence of each 33Leishmania stage on midgut gene expression. Two main patterns of sand fly gene expression 34 modulation were noticed. At early time points (days 1-4), more transcripts were down-regulated 35by Leishmania infection at large fold changes (> -32 fold). Among the down-regulated genes, the 36 transcription factor Forkhead/HNF-3 and hormone degradation enzymes were differentially 37 regulated on day 4 and appear to be the upstream regulators of nutrient transport, digestive 38 enzymes, and peritrophic matrix proteins. Conversely, at later time points (days 6 onwards), 39 most of the differentially expressed transcripts were up-regulated by small fold changes (< 32 40 fold), and the molecular function of such genes are associated with the metabolism of lipids and 41 detoxification of xenobiotics (P450). 42 Conclusion:Overall, it appears that Leishmania modulates sand fly gene expression early on in 43 order to overcome the barriers imposed by the midgut, yet it behaves like a commensal at later 44 time points, when modest midgut gene expression changes correlate with a massive amount of 45 parasites in the anterior midgut. 46

show abstract

Mosquito-specific microRNA-1890 targets the juvenile hormone-regulated serine protease JHA15 in the female mosquito gut

Cited by 53 publications

References 37 publications

Transcriptome-wide microRNA and target dynamics in the fat body during the gonadotrophic cycle of Aedes aegypti

Transcriptome-wide microRNA and target dynamics in the fat body during the gonadotrophic cycle of Aedes aegypti

Leishmania infection induces a limited differential gene expression in the sand fly midgut

Leishmania infection induces a limited differential gene expression in the sand fly midgut

Contact Info

Product

Resources

About