Mosquito pupicidal toxin production by Bacillus subtilis subsp. subtilis

Geetha, I.; Manonmani, A.M.

doi:10.1016/j.biocontrol.2007.10.007

Cited by 29 publications

(18 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The LC 50 values of L. auriculata (LC 50 of 8.6 Ϯ 0.6 g/ml) and P. moniliformis (LC 50 of 12.8 Ϯ 0.6 g/ml) extracts against SS Ae. aegypti pupae are comparable with those reported by Geetha and Manonmani (2008) for the crude mosquito pupicidal toxins of Bacillus subtilis (LC 50 of 11.8 Ϯ 0.6 g/ml).…”

Section: Resultssupporting

confidence: 88%

Toxicity of Brazilian Plant Seed Extracts to Two Strains of Aedes aegypti (Diptera: Culicidae) and Nontarget Animals

et al. 2011

View full text Add to dashboard Cite

Seed ethanolic extracts of 21 Brazilian plants were evaluated for ovicidal, larvicidal, and pupicidal activities against insecticide-susceptible (SS) and field-collected (FC) strains of Aedes aegypti (L.) (Diptera: Culicidae), as well as for their effects on nontarget organisms. Myracrodruon urundeuva Fr. Allemao extract was highly toxic to both mosquito strains. Schinopsis brasiliensis Engler extract showed low toxicity and was 38-68 times less toxic to Ae. aegypti larvae than was M. urundeuva extract. The pupicidal activity (LC50) of 14 plant seed extracts ranged between 9 and 433/g/ml, and toxicities were comparable to both mosquito strains. Piptadenia moniliformis Benth. and Luetzelburgia auriculata (Allemao) Ducke extracts showed the highest activities against pupae of FC and SS strains. None of the extracts showed 100% ovicidal activity. In addition, the active extracts did not show high acute toxicity to mice (LD50 > 1.5 g/kg), except that of Enterolobium contortisiliquum (Vell.) Morong. Most of the active extracts exhibited low toxicity against brine shrimp (Artemia sp.) nauplii. The extracts of M. urundeuva, P. moniliformis, and L. auriculata are promising sources of recognized classes of insecticidal compounds with good selectivity against immature stages of Ae. aegypti.

show abstract

Section: Resultssupporting

confidence: 88%

Toxicity of Brazilian Plant Seed Extracts to Two Strains of Aedes aegypti (Diptera: Culicidae) and Nontarget Animals

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Gupta and Vyas (1989) reported a strain of B. subtilis capable of causing mortality of larvae of Anopheles culicifacies, the primary vector of malaria in India. Moreover, effective formulation consisting of B. subtilis lipopeptide biosurfactant was also used for control of Drosophila melanogaster (Assie et al, 2002), Culex quinquefasciatus (Das and Mukherjee, 2006), Anopheles stephensi (Geetha and Manonmani, 2008) and Aedes aegypti (Geetha et al, 2010). Interestingly, B. subtilis has been reported as a biological control agent against Spodoptera littoralis (Abd El-Salam et al, 2011).…”

Section: Introductionmentioning

confidence: 97%

The impact of the Bacillus subtilis SPB1 biosurfactant on the midgut histology of Spodoptera littoralis (Lepidoptera: Noctuidae) and determination of its putative receptor

Ghribi

Abdelkefi-Mesrati

Boukedi

et al. 2012

Journal of Invertebrate Pathology

View full text Add to dashboard Cite

“…Mosquitocidal activity of B. subtilis is associated with the exotoxin unlike the other biolarvicides where the toxicity is associated with endotoxins. Further, mosquitocidal toxin production was found to be associated with vegetative growth of the organism rather than with sporulation (Geetha and Manonmani 2008). The mosquito pupicidal toxin produced by B. subtilis ssp.…”

Section: Introductionmentioning

confidence: 99%

Surfactin: a novel mosquitocidal biosurfactant produced by Bacillus subtilis ssp. subtilis (VCRC B471) and influence of abiotic factors on its pupicidal efficacy

Geetha¹,

Manonmani²

2010

Letters in Applied Microbiology

Self Cite

View full text Add to dashboard Cite

Aim: The rpoB gene of the mosquito pupicidal isolate Bacillus subtilis (VCRC B471) was amplified to confirm the subspecies as subtilis. The mosquito pupicidal activity expressed by the biosurfactant surfactin is novel, and hence, the influence of abiotic factors like pH, temperature of water and sunlight on its efficacy was studied under laboratory conditions. Methods and Results: The rpoB gene amplicon of the bacterium (c. 570 bp of) was sequenced (accession number: ). The relatedness of the bacterium to other members of the genus Bacillus was studied by tree construction, and the identity of VCRC B471 was confirmed as B. subtilis ssp. subtilis. The mosquito pupicidal activity exhibited by surfactin was found to be unaffected between pH 3–9, temperatures 25 and 37°C and exposure to sunlight/UV radiation. Further, the pupicidal activity of surfactin was not diminished after exposure to 121°C for 15 min, indicating its thermostable nature. Conclusions: VCRC B471 is confirmed as a strain of B. subtilis ssp. subtilis. The mosquitocidal toxin, surfactin produced by this bacterium being stable to UV and varied temperature, active at acidic and basic pH and temperatures between 25 and 42°C renders this molecule an interesting lead to be developed as a mosquitocidal agent. Significance and Impact of the Study: The mosquitocidal toxin, surfactin produced by B. subtilis ssp. subtilis (VCRC B471), being a biodegradable biosurfactant, exhibiting high stability to varied environmental conditions, can be used year round in breeding habitats and will be a prospective microbial toxin for use against mosquitoes.

show abstract

Mosquito pupicidal toxin production by Bacillus subtilis subsp. subtilis

Cited by 29 publications

References 8 publications

Toxicity of Brazilian Plant Seed Extracts to Two Strains of Aedes aegypti (Diptera: Culicidae) and Nontarget Animals

Toxicity of Brazilian Plant Seed Extracts to Two Strains of Aedes aegypti (Diptera: Culicidae) and Nontarget Animals

The impact of the Bacillus subtilis SPB1 biosurfactant on the midgut histology of Spodoptera littoralis (Lepidoptera: Noctuidae) and determination of its putative receptor

Surfactin: a novel mosquitocidal biosurfactant produced by Bacillus subtilis ssp. subtilis (VCRC B471) and influence of abiotic factors on its pupicidal efficacy

Contact Info

Product

Resources

About