Mosquito Larvicidal and Pupicidal Activity of Tephrosia purpurea Linn. (Family: Fabaceae) and Bacillus sphaericus against, Dengue Vector, Aedes aegypti

Abstract

Objective:The bio-efficacy of Tephrosia purpurea leaf extract and bacterial insecticide, Bacillus sphaericus larvicidal activity was assessed against the first to fourth instars larvae of Aedes aegypti, under the laboratory conditions. Methods: The plant material was shade dried at room temperature and powdered coarsely. T. purpurea and B. sphaericus show the various concentrations of larvicidal and pupicidal activity against various instars larvae of A. aegypti. Results: The LC 50 of T. purpurea against the first to fourth instars larvae were 139. 24, 176.24, 219.28, 256.27, and 326.29 A. aegypti in all the tested concentrations than the individuals and clearly established that there is a substantial amount of synergist act. Conclusion: The present study reported that both T. purpurea and B. sphaericus materials could serve as a potential larvicidal agent. Since, A. aegypti is a container breeder vector mosquito this user and eco-friendly and low-cost vector control strategy could be a viable solution to the existing dengue disease burden. Therefore, this study provides first report on the mosquito larvicidal activity the combined effect of T. purpurea leaf extract and B. sphaericus against as target species of A. aegypti.

Larvicidal and Pupicidal activity of Clerodendrum philippinum Schauer Leaf Extracts against Anopheles stephensi and Aedes aegypti

“…Due to aquatic condition, they cause adverse effects on the environment and human health 33 and hence, this finding not only helps to control the spreading of mosquito but also biodegradable and easily available in low cost. 34 15 The results so obtained also having similarities to Amir et al who have taken the direct leaf and stem extracts of Parthenium hysterophorus against A. aegypti and confirmed as potential natural larvicidal agent. 12 In our present study, the ethanol and methanol extracts of C. philippinum possessed higher larvicidal and pupicidal activities against An.…”

“…They possess rich source of secondary metabolites which having insecticidal activity (antibacterial, antifungal and larvicidal potential) and is possible due to presence of active phenols, alkaloids, terpenoids, coumarins, polysaccharides and flavonoids etc. [12][13] In fact, many studies have been conducted to find out the effectiveness of different plant extracts against larvicidal and pupicidal activity of mosquito, 4,12,[14][15][16][17] but there is no such report against Clerodendrum philippinum.…”

Plants in the Genus Tephrosia: Valuable Resources for Botanical Insecticides

“…The insecticidal efficacy was attributed to the presence of compounds like rotenone and hexadecanoic acid. T. purpurea leaf extract was able to control first to fourth instars larvae and pupae of A. aegypti [ 82 ]. Furthermore, the whole plant extract of T. purpurea was tested for its larvicidal activity against the larvae of Culex quinquefasciatus .…”

Studi Toksisitas: Ekstrak Metanol Bonggol Pisang Ambon (Musa acuminata L. cv. Gros Michel) terhadap Aedes aegypti (Diptera: Culcidae)

“…Efek toksik ekstrak metanol bonggol pisang ambon pada pupa lebih rendah, dibandingkan telur dan larva, yaitu hanya mampu menyebabkan kematian sebesar 46,67% pupa dan memberikan nilai LC50 yang lebih besar yaitu 1075,296 ppm. Berbeda dengan studi terhadap pupa dari tanaman lain pada umumnya, diantaranya temuan Venkadachalam, et al 50 Beberapa penelitian sebelumnya mengungkapkan bahwa dari semua tahap perkembangan nyamuk pradewasa, pupa merupakan fase yang paling tahan terhadap insektisida nabati, yang ditunjukkan dengan nilai LC50 yang besar 43,44 . Ketahanan tersebut mungkin disebabkan karena pupa merupakan tahap pradewasa yang tidak makan, sehingga mengurangi peluang mereka menelan senyawa toksik ke dalam sistem tubuh, terutama sistem pencernaan 52,53 .…”