Mosquito (Diptera: Culicidae) Larvicidal and Ovicidal Properties of Extracts from<i>Streptomyces vinaceusdrappus</i>(S12-4) Isolated from Soils<sup>1</sup>

Ganesan, Pathalam; Jackson, Amalraj; David, Rajendran Host Antony; Sivanandhan, Subramaniyan; Gandhi, Munusamy Rajiv; Paulraj, Michael Gabriel; Al-Dhabi, Naïf Abdullah; Ignacimuthu, S.

doi:10.18474/jes17-21.1

Cited by 8 publications

(4 citation statements)

References 12 publications

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“…However, the findings of this study suggest that the bio-larvicidal activity of the GA 9 extract is concentration-dependent and varies across different mosquito species. 26,41,46,47 This study also investigated the effect of Streptomyces spp GA 9 exudates on Aedes larva. The results showed that the activity level at concentrations of 0.6 mg/ml and 0.8 mg/ml resulted in a 90% mortality rate, while the LC50 of the extract at 0.4 mg/ml had a 70% mortality rate.…”

Section: Resultsmentioning

confidence: 99%

“…These findings have important implications for developing bio-larvicidal agents and highlight the potential of Streptomyces spp GA 9 exudates as effective mosquito larvicides. [52][53][54][55][56] The mortality rate of brine shrimps in response to the Streptomyces spp GA 9 extract was moderate, with the highest concentration (0.8 mg/ml) resulting in the highest mortality rate of 20%. Interestingly, the percentage mortality for concentrations of 0.2 mg/ml, 0.4 mg/ml, and 0.6 mg/ml was 10%, which is relatively lower than the toxicity of the positive control.…”

Section: Resultsmentioning

confidence: 99%

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Bio-larvicidal and Cytotoxic Activity of forest Streptomyces Isolate GA9 Against Some Mosquito Larvae

2024

TJNPR

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An antibiotic-producing Streptomyces spp. was isolated from forest soil and assessed for its biolarvicidal and cytotoxic activity against various mosquito species (Aedes spp, Anopheles spp, Culex spp, and Mansonia spp) and Brime shrimps. Active biocides were extracted using a modified solid state fermentation process. Mosquito larvae were exposed to different concentrations of the ethanolic extract of the Streptomyces exudates for 24 hours. The extract's cytotoxicity was also evaluated using Brime shrimp. The results revealed a significant mortality rate among all four important vector mosquitoes following treatment with the extract. The LC50 and LC90 values of the extract were 0.40 mg/ml and 0.80 mg/ml for Anopheles spp, 0.20 mg/ml and 0.60 mg/ml for Culex spp, 0.40 mg/ml and 0.60 mg/ml for Aedes spp, and 0.20 mg/ml and 0.60 mg/ml for Mansonia spp, respectively. The Minimum larvicidal concentration values were 0.2 mg/ml for Aedes spp, Anopheles spp, Culex spp, and Mansonia spp. The extract showed a low degree of cytotoxicity, with an average mortality rate of only 20% at 0.8 mg/ml of the extract, upon testing with Brime shrimp. In conclusion, the aqueous extract of forest Streptomyces spp. exhibited high bio-larvicidal activity against Aedes spp, Anopheles spp, Culex spp, and Mansonia spp larvae, suggesting its potential as an environmentally friendly approach to mosquito control. This study represents an initial step toward supplementing eco-friendly nontoxic microbe-based bioinsecticides for synthetic insecticides against medically significant mosquitoes.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Bio-larvicidal and Cytotoxic Activity of forest Streptomyces Isolate GA9 Against Some Mosquito Larvae

2024

TJNPR

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“…The fermentation and extraction of bioactive compounds from Streptomyces sp. KSF103 in this study was performed following the methods of Ganesan, et al 25 and Thenmozhi, et al 26 with suitable modifications. Streptomyces sp.…”

Section: Methodsmentioning

confidence: 99%

Insecticidal activities of Streptomyces sp. KSF103 ethyl acetate extract against medically important mosquitoes and non-target organisms

Amelia-Yap

Low

Saeung

et al. 2023

Sci Rep

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A potentially novel actinobacterium isolated from forest soil, Streptomyces sp. KSF103 was evaluated for its insecticidal effect against several mosquito species namely Aedes aegypti, Aedes albopictus, Anopheles cracens and Culex quinquefasciatus. Mosquito larvae and adults were exposed to various concentrations of the ethyl acetate (EA) extract for 24 h. Considerable mortality was evident after the EA extract treatment for all four important vector mosquitoes. Larvicidal activity of the EA extract resulted in LC50 at 0.045 mg/mL and LC90 at 0.080 mg/mL for Ae. aegypti; LC50 at 0.060 mg/mL and LC90 at 0.247 mg/mL for Ae. albopictus; LC50 at 2.141 mg/mL and LC90 at 6.345 mg/mL for An. cracens; and LC50 at 0.272 mg/mL and LC90 at 0.980 mg/mL for Cx. quinquefasciatus. In adulticidal tests, the EA extract was the most toxic to Ae. albopictus adults (LD50 = 2.445 mg/mL; LD90 = 20.004 mg/mL), followed by An. cracens (LD50 = 5.121 mg/mL; LD90 = 147.854 mg/mL) and then Ae. aegypti (LD50 = 28.873 mg/mL; LD90 = 274.823 mg/mL). Additionally, the EA extract exhibited ovicidal activity against Ae. aegypti (LC50 = 0.715 mg/mL; LC90 = 6.956 mg/mL), Ae. albopictus (LC50 = 0.715 mg/mL; LC90 = 6.956 mg/mL), and An. cracens (LC50 = 0.715 mg/mL; LC90 = 6.956 mg/mL), evaluated up to 168 h post-treatment. It displayed no toxicity on the freshwater microalga Chlorella sp. Beijerinck UMACC 313, marine microalga Chlorella sp. Beijerinck UMACC 258 and the ant Odontoponera denticulata. In conclusion, the EA extract showed promising larvicidal, adulticidal and ovicidal activity against Ae. aegypti, Ae. albopictus, An. cracens, and Cx. quinquefasciatus (larvae only). The results suggest that the EA extract of Streptomyces sp. KSF103 has the potential to be used as an environmental-friendly approach in mosquito control. The current study would serve as an initial step toward complementing microbe-based bioinsecticides for synthetic insecticides against medically important mosquitoes.

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“…Ganesan et al, 2018).It is the first report of L. fusiformis from the soil of Western Ghats, Kerala having mosquitocidal activity. It is a naturally occurring bacterium in the family Bacillaceae and was first discovered in 1901…”

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confidence: 96%

Lysinibacillus fusiformis: A novel mosquitocidal bacterium isolated from Western Ghats, Kerala, India

Aneha,

Bora,

Hemaladkshmi

et al. 2023

Entomon

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An extensive field survey was carried out from December 2021 to January 2022 for the collection of soil samples in the Western Ghat region of the Wayanad district of Kerala, India, to isolate potent novel mosquitocidal bacteria which could be used as efficient formulations to control mosquito vectors. Several bacterial colonies were isolated and screened, for mosquitocidal activity. Toxicity assay showed that only one bacterium isolated from forest loamy soil had promising larvicidal activity against Culex quinquefasciatus the lymphatic filariasis vector (LC50: 0.03 mg L-1 & LC90: 0.07 mg L-1) and moderate activity was shown against the dengue vector, Aedes aegypti (LC50: 1.03 mg L-1 & LC90: 1.8 mg L-1). The bacterium was identified as Lysnibacillus fusiformis by a phylogenetic tree constructed using 16S rRNA genome sequencing. This is the first report that L. fusiformis isolated from the forest loamy soil of the Western Ghats, Kerala, which showed proficient mosquitocidal activity against disease-transmitting mosquito vectors.

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Mosquito (Diptera: Culicidae) Larvicidal and Ovicidal Properties of Extracts fromStreptomyces vinaceusdrappus(S12-4) Isolated from Soils¹

Cited by 8 publications

References 12 publications

Bio-larvicidal and Cytotoxic Activity of forest Streptomyces Isolate GA9 Against Some Mosquito Larvae

Bio-larvicidal and Cytotoxic Activity of forest Streptomyces Isolate GA9 Against Some Mosquito Larvae

Insecticidal activities of Streptomyces sp. KSF103 ethyl acetate extract against medically important mosquitoes and non-target organisms

Lysinibacillus fusiformis: A novel mosquitocidal bacterium isolated from Western Ghats, Kerala, India

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Mosquito (Diptera: Culicidae) Larvicidal and Ovicidal Properties of Extracts fromStreptomyces vinaceusdrappus(S12-4) Isolated from Soils1

Cited by 8 publications

References 12 publications

Bio-larvicidal and Cytotoxic Activity of forest Streptomyces Isolate GA9 Against Some Mosquito Larvae

Bio-larvicidal and Cytotoxic Activity of forest Streptomyces Isolate GA9 Against Some Mosquito Larvae

Insecticidal activities of Streptomyces sp. KSF103 ethyl acetate extract against medically important mosquitoes and non-target organisms

Lysinibacillus fusiformis: A novel mosquitocidal bacterium isolated from Western Ghats, Kerala, India

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Mosquito (Diptera: Culicidae) Larvicidal and Ovicidal Properties of Extracts fromStreptomyces vinaceusdrappus(S12-4) Isolated from Soils¹