Mosquito carboxylesterases: a review of the molecular biology and biochemistry of a major insecticide resistance mechanism

Hemingway, Janet; Karunaratne, S.H.P.P.

doi:10.1046/j.1365-2915.1998.00082.x

Cited by 279 publications

(198 citation statements)

References 79 publications

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“…The other esetrases were detected almost in all collected samples with frequency which vaired between 0.03 and 0.48. Esterases play an important role in the resistance of several insects in the world [25][26][27][28][29][30][31]. Our results confirms the previous results of Ben Cheikh et al [28] which reported the existence of a correlation between the frequency of individuals possessing the Ace-1R allele and those overproduced esterases A and B among the Tunisian populations of Culex pipiens.…”

Section: Hereditary Geneticssupporting

confidence: 82%

Pirimiphos-Methyl Resistance Status of Field Populations of Culex pipiens (Diptera: Culicidae) From Grand Tunis Area, Northeast Tunisia

Tabbabi¹,

Daaboub²,

Laamari³

et al. 2017

Hereditary Genet

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Mosquito species are responsible for the transmission of many parasitic diseases and their control leads to the phenomenon of resistance to insecticides. Five Culex pipiens samples were collected from various localities of Grand Tunis area, Northeast Tunisia, between June 2003 and November 2005. All the studied samples were resistant to pirimiphos methyl (organophosphorus insecticide). Our results showed the implication of insensitive acetylcholinesterase and overproduced esterases in the résistance of the Tunisian populations of Culex pipiens. Results were discussed in relation to resistance mechanism.

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Section: Hereditary Geneticssupporting

confidence: 82%

Pirimiphos-Methyl Resistance Status of Field Populations of Culex pipiens (Diptera: Culicidae) From Grand Tunis Area, Northeast Tunisia

Tabbabi¹,

Daaboub²,

Laamari³

et al. 2017

Hereditary Genet

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“…COE genes, which play an important role in the metabolism of a wide range of xenobiotics associated with plants and insecticides 22,[28][29][30] , also showed large expansions of lepidopteran and α classes ( Table 1, Supplementary Fig. 6a and Supplementary Table 15).…”

Section: Nature Ecology and Evolutionmentioning

confidence: 99%

Genomic adaptation to polyphagy and insecticides in a major East Asian noctuid pest

et al. 2017

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The tobacco cutworm, Spodoptera litura, is among the most widespread and destructive agricultural pests, feeding on over 100 crops throughout tropical and subtropical Asia. By genome sequencing, physical mapping and transcriptome analysis, we found that the gene families encoding receptors for bitter or toxic substances and detoxification enzymes, such as cytochrome P450, carboxylesterase and glutathione-S-transferase, were massively expanded in this polyphagous species, enabling its extraordinary ability to detect and detoxify many plant secondary compounds. Larval exposure to insecticidal toxins induced expression of detoxification genes, and knockdown of representative genes using short interfering RNA (siRNA) reduced larval survival, consistent with their contribution to the insect’s natural pesticide tolerance. A population genetics study indicated that this species expanded throughout southeast Asia by migrating along a South India–South China–Japan axis, adapting to wide-ranging ecological conditions with diverse host plants and insecticides, surviving and adapting with the aid of its expanded detoxification systems. The findings of this study will enable the development of new pest management strategies for the control of major agricultural pests such as S. litura.

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“…The most prevalent amplified esterase genes are estα2" and estβ2", which occur worldwide [1,14,15]. Some insects have variants of estβ1 (alleles of estβ2") that might or might not have a co-amplified estα3 [16].…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of gene amplification in insecticide-resistant Culex mosquitoes

Paton

Karunaratne

Giakoumaki

et al. 2000

Biochemical Journal

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The amplification of carboxylesterase structural genes followed by their overexpression is the most common mechanism of resistance to organophosphorus insecticides in Culex mosquitoes. Most resistant Culex quinquefasciatus mosquitoes have coamplified estα2" and estβ2" genes. Recently, Southern, DNA dotblot analysis and phosphorimaging technology were used to quantify the est gene copy number in aphids and mosquitoes. Although more accurate than autoradiography, this method relies on probe hybridization, which can be variable. We have directly measured gene and mRNA copy number by using realtime quantitative PCRs in mosquitoes. The acquisition of fluorescence from incorporation of the double-strand-specific dye SYBR GreenI into a PCR product once per cycle is used to provide an absolute quantification of the initial template copy number. Thus it has been possible to show that estα2" and estβ2"

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Mosquito carboxylesterases: a review of the molecular biology and biochemistry of a major insecticide resistance mechanism

Cited by 279 publications

References 79 publications

Pirimiphos-Methyl Resistance Status of Field Populations of Culex pipiens (Diptera: Culicidae) From Grand Tunis Area, Northeast Tunisia

Pirimiphos-Methyl Resistance Status of Field Populations of Culex pipiens (Diptera: Culicidae) From Grand Tunis Area, Northeast Tunisia

Genomic adaptation to polyphagy and insecticides in a major East Asian noctuid pest

Quantitative analysis of gene amplification in insecticide-resistant Culex mosquitoes

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