Mosquito-associated Dengue Virus, Key West, Florida, USA, 2010

Graham, Amanda S.; Pruszynski, Catherine A.; Hribar, Lawrence J.; DeMay, David J.; Tambasco, Adriane N.; Hartley, Anne E.; Fussell, Edsel M.; Michael, Scott F.; Isern, Sharon

doi:10.3201/eid1711.110419

Cited by 43 publications

(33 citation statements)

References 6 publications

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“…The clade containing Central American strains was separated from the clade containing South American and recent Caribbean strains with strong bootstrap support. The Key West 2010 strains grouped with Central American strains, as previously reported (9, 10). The Martin County DENV-1 strain grouped most closely with viruses that have recently appeared in the Caribbean from South American origins.…”

Section: Resultssupporting

confidence: 76%

“…In 2010, an additional sixty-three cases of locally acquired DENV were reported in Monroe County and one case each in Miami-Dade and Broward Counties. The same strain of DENV serotype 1 (DENV-1)was isolated from both mosquitoes and patients in Monroe County, confirming local transmission (9, 10). No further cases of locally acquired DENV have been reported in Monroe County since 2010, suggesting that DENV had been extirpated from the local vector population in that location.…”

Section: Introductionmentioning

confidence: 71%

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Origin of the dengue virus outbreak in Martin County, Florida, USA 2013

et al. 2014

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After a 75-year absence from Florida, substantial local transmission of dengue virus (DENV) occurred in Key West, Monroe County, Florida in 2009 and continued in 2010. The outbreak culminated in 85 reported cases. In 2011 and 2012, only isolated cases of local DENV transmission were reported in Florida, none were reported in Key West. In 2013, a new outbreak occurred, but this time in Martin County about 275 miles North of Key West with 22 reported cases. As the Key West and Martin County outbreaks involved DENV serotype 1 (DENV-1), we wanted to investigate whether the same strain or a different strain of DENV was responsible for the outbreaks. In this study, we report the sequence and phylogenetic analysis of the E generegion from a patient diagnosed with dengue in Martin County. Our results indicate that the 2013 Martin County DENV-1 strain is distinct from the 2009–2010 Key West DENV-1 and that it is most closely related to viruses from a recent expansion of South American DENV-1 strains into the Caribbean. We conclude that the 2013 Martin County outbreak was the result of a new introduction of DENV-1 in Florida.

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Section: Resultssupporting

confidence: 76%

Section: Introductionmentioning

confidence: 71%

Origin of the dengue virus outbreak in Martin County, Florida, USA 2013

et al. 2014

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“…aegypti and humans was documented within subtropical Key West in Monroe County, FL, in 2009 and 2010 for the first time in over 50 yr, resulting in 27 and 63 human cases in 2009 and 2010, respectively (Centers for Disease Control [CDC] 2010, Graham et al 2011). In addition to the confirmed cases, a random serosurvey conducted in September 2009 suggested that ≈600– 1,000 Key West residents were infected with DENV during the 2009 outbreak (Radke et al 2012).…”

mentioning

confidence: 99%

Vertical Transmission of Key West Dengue-1 Virus by <I>Aedes aegypti</I> and <I>Aedes albopictus</I> (Diptera: Culicidae) Mosquitoes From Florida

2013

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Following the 2009 and 2010 dengue-1 (DENV-1) outbreaks in Key West, FL, we used Florida Aedes aegypti (L.) mosquitoes and DENV-1 isolated from Key West in 2010 to test the hypothesis that if the 2009 and 2010 DENV-1 genome sequences are similar, then vertical transmission of DENV-1 from infected Ae. aegypti female mosquitoes to their eggs could have served as an interepidemic reservoir between outbreaks. We also investigated the ability of Florida Aedes albopictus (Skuse) mosquitoes to vertically transmit DENV-1. In addition, we determined the rates of infection and dissemination of these Florida mosquito species for DENV-1 and the effect of DENV-1 infection on oviposition success and number of mosquito eggs laid by females. Vertical transmission of DENV-1 was documented, with rates of 11.11% (2 out of 18) for Ae. albopictus and 8.33% (3 out of 36) for Ae. aegypti. Approximately 93% (111 out of 119) of Ae. aegypti that fed on DENV-1 in blood became infected, and 80% (89 out of 111) of infections were disseminated. Similarly, 93% of Ae. albopictus became infected (53 out of 57), and 85% (45 out of 53) of infections were disseminated. No significant differences were detected in numbers of eggs laid by either species after imbibing DENV-1 in blood, suggesting little cost of infection on number of eggs laid. Our results demonstrate that Florida Ae. aegypti and Ae. albopictus mosquitoes are competent vectors for DENV-1, whose maintenance between the 2009 and 2010 Key West outbreaks may have been facilitated by vertical transmission.

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“…Dengue has been found to be the most frequent cause of febrile illness among US travelers returning from Asia, Latin America and the Caribbean (Freedman et al , 2006; Mohammed et al , 2010b; Sharp et al , 2012). In addition, outbreaks of dengue occur sporadically in non-endemic areas where the mosquito vectors exist (Brunkard et al , 2007; Graham et al , 2011; Radke et al , 2012; Ramos et al , 2008), Hawaii (Effler et al , 2005), and Florida, (Radke et al , 2012). Meanwhile, WNV infections have been reported throughout the continental United States.…”

Section: Flavivirus Epidemiology and Global Dispersalmentioning

confidence: 99%

Flaviviruses, an expanding threat in public health: focus on dengue, West Nile, and Japanese encephalitis virus

2014

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The flaviviruses Dengue, West Nile, and Japanese encephalitis represent three major mosquito-borne viruses worldwide. These pathogens impact the lives of millions of individuals and potentially could affect non-endemic areas already colonized by mosquito vectors. Unintentional transport of infected vectors (Aedes and Culex sp), traveling within endemic areas, rapid adaptation of the insects into new geographic locations, climate change, and lack of medical surveillance have greatly contributed to the increase in flaviviral infections worldwide. The mechanisms by which flaviviruses alter the immune and the central nervous system have only recently been examined despite the alarming number of infections, related deaths, and increasing global distribution. In this review, we will discuss the expansion of the geographic areas affected by flaviviruses, the potential threats to previously unaffected countries, the mechanisms of pathogenesis, and the potential therapeutic interventions to limit the devastating consequences of these viruses.

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Mosquito-associated Dengue Virus, Key West, Florida, USA, 2010

Cited by 43 publications

References 6 publications

Origin of the dengue virus outbreak in Martin County, Florida, USA 2013

Origin of the dengue virus outbreak in Martin County, Florida, USA 2013

Vertical Transmission of Key West Dengue-1 Virus by <I>Aedes aegypti</I> and <I>Aedes albopictus</I> (Diptera: Culicidae) Mosquitoes From Florida

Flaviviruses, an expanding threat in public health: focus on dengue, West Nile, and Japanese encephalitis virus

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