Mosaic patterns of transgene expression in plants

Маренкова, Т. В.; Loginova, D. B.; Дейнеко, Е. В.

doi:10.1134/s1022795412030088

Cited by 10 publications

(7 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The evidence from the literature points at transgene silencing, or partial cell-to-cell gene silencing, as a major determinant of the uneven and patchy patterns of transgene expression in plants (Day et al 2000, De Buck et al 2007, Marjanac et al 2009. Whereas this phenotype can result from the insertion of a single copy transgene (Day et al 2000), irregular patterns of transgene expression have also been linked to transgenes in multiple copies (Reddy et al 2003, Marenkova et al 2012. Multiple copy transgenes have consistently been reported in transgenic wheat generated by particle bombardment when using supercoiled (as in the present study) or linearized whole plasmid (Sivamani et al 2002, Rooke et al 2003, as compared to gene constructs lacking vector backbone sequences (Yao et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Characterization of the high-affinity phosphate transporter PHT1;4 gene promoter of Arabidopsis thaliana in transgenic wheat

et al. 2017

View full text Add to dashboard Cite

The root specificity and phosphate (Pi) deficiency responsiveness of high-affinity phosphate transporter (PHT1) genes point to their promoters as a sustainable system to drive Pi acquisition-related transgenes in plants. In this study, a 3-kb promoter of the AtPHT1;4 gene from Arabidopsis thaliana fused to the -glucuronidase (GUS) reporter gene was biolistically introduced into wheat (Triticum aestivum L.) and functionally characterized in transgenic plants grown in hydroponics and in pots with soil under various Pi supply rates. From among 27 T 1 progeny derived from 250 T 0 , four transgenic lines reached T 3 , with two of them showing detectable GUS activity in the roots of T 4 plants. An unusually high number of transgene insertions characterized these transgenic lines, along with an irregular pattern of histochemical GUS staining and weak GUS activity. GUS expression driven by AtPHT1;4 was consistently higher under most assay conditions, as it was unaffected by 0 to 0.5 mM Pi in hydroponically grown plants, as well as by 16 to 20 mg(P) kg -1 (soil) in potted plants. Raising the soil P up to or above 40 mg kg -1 significantly down-regulated the quantity of GUS transcripts. These results show that the responsiveness of the AtPHT1;4 promoter to Pi availability in transgenic wheat was restricted to soil-grown plants, which highlighted the relevance of the substrate and Pi supply rates in assessing molecular responses to Pi deficiency.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of the high-affinity phosphate transporter PHT1;4 gene promoter of Arabidopsis thaliana in transgenic wheat

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Methylation is an epigenetic modification that affects both the folding of the chromatin and the transcription. It should be noted that a wide variation in the expression of recombinant genes is observed during the creation of transgenic plants in both original transformants and among the offspring in subsequent generations [34]. Thus, the instability of transgenic expression in plants may be associated with both complete loss of expression in all plant cells, and with a partial loss that is manifested as alternating regions with active and inactive transgene in cells of the same somatic tissue.…”

Section: Discussionmentioning

confidence: 99%

“…This is consistent with the results of other authors. Thus, significant differences in the methylation of CpG-rich sequences from plants (barley) and human that were randomly integrated into the genome of tobacco plants were demonstrated [34]. The authors showed that CpG-rich sequences from the human genome were not methylated, whereas plant sequences were subjected to methylation, i.e., CpG-islands of the human genome were protected from methylation in the context of plant chromosomes.…”

Section: Discussionmentioning

confidence: 99%

Efficient expression of a heterologous gene in plants depends on the nucleotide composition of mRNA’s 5'-region

Tyurin

Кабардаева

Gra

et al. 2016

Russ J Plant Physiol

View full text Add to dashboard Cite

The contribution of nucleotide composition of mRNA 5'-region to the efficiency of expression at transcriptional and translational levels was studied in transgenic tobacco plants (Nicotiana tabacum L., cultivar Petit Havana) using a thermostable lichenase reporter gene. Synthetic sequence that contains CG-rich motifs, typical for 5'-region of plant genes, identified in silico, was constructed. Transgenic plant lines of N. tabacum were obtained; they contain thermostable lichenase reporter gene that is under control of the constitutive 35S RNA CaMV promoter and additional regulatory element: synthetic CG-rich sequence, which functions as the 5'-UTR (untranslated region) of the reporter gene mRNA or as 5'-region of the hybrid gene coding sequence, wherein the synthetic sequence is fused with the sequence of the reporter gene in its reading frame. Results of the comparative analysis of mRNA and protein levels in the obtained lines of transgenic plants showed that the synthetic CG-rich sequence significantly increases the level of transcription of the reporter gene and appear to have no negative effect on the efficiency of reporter mRNA translation, which may be due to the peculiarities of its nucleotide composition and structure, namely, due to the presence of motifs that are specific for 5'-regions of plant genes, as well as due to the properties of the secondary structure-the absence of hairpin structures with a high energy of formation. It was experimentally confirmed for the first time that the 5'-region of the genes with a high content of CpG dinucleotides can help to increase the transcription level of genes in plants.

show abstract

“…Since all the infected transgenic lines were PCR checked, the variable response levels among these transgenic lines could be due to the different integration patterns that could have occurred for each line. Variation in expression and stability which is reported to be caused by environmental effects, promoter methylation, inter-loci interactions and gene silencing can also be responsible for the differences observed (Marenkova et al, 2012). The lines generated as part of this study were evaluated for a single growth cycle and the performance of these genes in ratoon crops was not assessed.…”

Section: Assessing Fusarium Wilt Tolerancementioning

confidence: 99%

Inhibition of cell death as an approach for development of transgenic resistance against Fusarium wilt disease

Magambo¹,

Khanna²,

Arinaitwe³

et al. 2016

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Fusarium oxysporum f. sp. cubense (Foc) is one of the major threats to dessert banana (Musa spp.) production. In Uganda, 'Sukali Ndiizi' is one of the most popular dessert banana cultivars and it is highly susceptible to Fusarium wilt. Development of resistant cultivars through transgenic approaches has shown to offer one of the most effective control options for most diseases. The transgenic approaches for providing plant disease resistance have mainly been through either enzymatic destruction of pathogen structures, neutralization of pathogen and its products or production of metabolites that eventually kill the pathogen. However in recent years, methods that prevent cell death of host plant after infection especially for necrotrophic pathogens like F. oxysporum have registered success in providing resistance in several crops. We investigated whether the transgenic expression of a programmed cell death inhibition gene in Sukali Ndiizi could be used to confer Fusarium resistance to Foc race 1. Embryogenic cell suspensions of cv. 'Sukali Ndiizi, were stably transformed with a synthetic, plant-codon optimise mCed-9 gene. Twenty-eight independently transformed plant lines were regenerated. The lines were inoculated with Foc race 1 and observed for 13 weeks in small-plant glasshouse. Three transgenic lines showed significantly lower internal and external disease symptoms than the wild-type susceptible 'Sukali Ndiizi' banana plants used as controls. This is the first report from Africa on the generation of Fusarium wilt tolerant transgenic 'Sukali Ndiizi', a very popular but rapidly diminishing African dessert banana.

show abstract

Mosaic patterns of transgene expression in plants

Cited by 10 publications

References 62 publications

Characterization of the high-affinity phosphate transporter PHT1;4 gene promoter of Arabidopsis thaliana in transgenic wheat

Characterization of the high-affinity phosphate transporter PHT1;4 gene promoter of Arabidopsis thaliana in transgenic wheat

Efficient expression of a heterologous gene in plants depends on the nucleotide composition of mRNA’s 5'-region

Inhibition of cell death as an approach for development of transgenic resistance against Fusarium wilt disease

Contact Info

Product

Resources

About