Morusinol Extracted from &lt;i&gt;Morus Alba&lt;/i&gt; Inhibits Arterial Thrombosis and Modulates Platelet Activation for the Treatment of Cardiovascular Disease

Lee, Jung‐Jin; Yang, Hyun; Yoo, Yeong Min; Hong, Seong Su; Lee, Dong‐Ho; Lee, Hyun Jung; Lee, Hak Ju; Myung, Chang‐Seon; Choi, Kyung Chul; Jeung, Eui Bae

doi:10.5551/jat.10058

Cited by 37 publications

(21 citation statements)

References 26 publications

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“…This antiplatelet or antithrombotic effect of M. alba leaves has never been described before, though an antiplatelet effect has been described with its barks [23]. …”

Section: Introductionmentioning

confidence: 99%

Antiplatelet Activity of Morus alba Leaves Extract, Mediated via Inhibiting Granule Secretion and Blocking the Phosphorylation of Extracellular‐Signal‐Regulated Kinase and Akt

Kim

Rhee

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Ethnopharmacological Relevance. Morus alba L. leaves (MAE) have been used in fork medicine for the treatment of beriberi, edema, diabetes, hypertension, and atherosclerosis. However, underlying mechanism of MAE on cardiovascular protection remains to be elucidated. Therefore, we investigated whether MAE affect platelet aggregation and thrombosis. Materials and Methods. The anti-platelet activity of MAE was studied using rat platelets. The extent of anti-platelet activity of MAE was assayed in collagen-induced platelet aggregation. ATP and serotonin release was carried out. The activation of integrin α IIb β 3 and phosphorylation of signaling molecules, including MAPK and Akt, were investigated with cytofluorometer and immunoblotting, respectively. The thrombus formation in vivo was also evaluated in arteriovenous shunt model of rats. Results. HPLC chromatographic analysis revealed that MAE contained rutin and isoquercetin. MAE dose-dependently inhibited collagen-induced platelet aggregation. MAE also attenuated serotonin secretion and thromboxane A2 formation. In addition, the extract in vivo activity showed that MAE at 100, 200, and 400 mg/kg significantly and dose-dependently attenuated thrombus formation in rat arterio-venous shunt model by 52.3% (P < 0.001), 28.3% (P < 0.01), and 19.1% (P < 0.05), respectively. Conclusions. MAE inhibit platelet activation, TXB2 formation, serotonin secretion, aggregation, and thrombus formation. The plant extract could be considered as a candidate to anti-platelet and antithrombotic agent.

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“…This antiplatelet or antithrombotic effect of M. alba leaves has never been described before, though an antiplatelet effect has been described with its barks [23]. …”

Section: Introductionmentioning

confidence: 99%

Antiplatelet Activity of Morus alba Leaves Extract, Mediated via Inhibiting Granule Secretion and Blocking the Phosphorylation of Extracellular‐Signal‐Regulated Kinase and Akt

Kim

Rhee

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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“…Since transition metals facilitate oxidative radical formation, inducing cellular and tissue injuries as well as endothelial cell damage leading to thrombosis, application of ferric chloride (FeCl 3 ) to arteries has been used as a model to assess the effectiveness of anti-thrombotic blood flow enhancers [9]. Nattokinase, a thrombolytic enzyme rich in 'Natto', an Oriental traditional food, is produced by Bacillus subtilis Natto during fermentation of soybeans [10-12].…”

mentioning

confidence: 99%

“…Platelet-rich plasma (PRP) was obtained by centrifugation at 230 g for 10 min. Platelets were sedimented by centrifugation of the PRP at 800 g for 15 min and washed with a HEPES buffer (pH 6.5) [9,14]. The washed platelets were resuspended (4×10 8 cells/mL) in the HEPES buffer (pH 7.4).…”

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confidence: 99%

Nattokinase improves blood flow by inhibiting platelet aggregation and thrombus formation

Jang

Kim²,

Cai

et al. 2013

Lab Anim Res

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The effects of nattokinase on the in vitro platelet aggregation and in vivo thrombosis were investigated in comparison with aspirin. Rabbit platelet-rich plasma was incubated with nattokinase and aggregation inducers collagen and thrombin, and the platelet aggregation rate was analyzed. Nattokinase significantly inhibited both the collagen- and thrombin-induced platelet aggregations. Nattokinase also reduced thromboxane B2 formation from collagen-activated platelets in a concentration-dependent manner. Rats were orally administered with nattokinase for 1 week, and their carotid arteries were exposed. Arterial thrombosis was induced by applying 35% FeCl3-soaked filter paper for 10 min, and the blood flow was monitored with a laser Doppler probe. Nattokinase delayed the FeCl3-induced arterial occlusion in a dose-dependent manner, doubling the occlusion time at 160 mg/kg. In addition, a high dose (500 mg/kg) of nattokinase fully prevented the occlusion, as achieved with aspirin (30 mg/kg). The results indicate that nattokinase extracted from fermented soybean inhibit platelet aggregation by blocking thromboxane formation, and thereby delay thrombosis following oxidative arterial wall injury. Therefore, it is suggested that nattokinase could be a good candidate without adverse effects for the improvement of blood flow.

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“…Platelets were sedimented by centrifugation of the PRP at 350 g and washed with tyrode buffer. The washed platelets were resuspended in tyrode buffer and adjusted to 3×10 8 platelets/mL [2223]. …”

Section: Methodsmentioning

confidence: 99%

Blood flow-improving activity of methyl jasmonate-treated adventitious roots of mountain ginseng

et al. 2017

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Ginsenosides from Panax ginseng are well known for their diverse pharmacological effects including antithrombotic activity. Since adventitious roots of mountain ginseng (ARMG) also contain various ginsenosides, blood flow-improving effects of the dried powder and extract of ARMG were investigated. Rats were orally administered with dried powder (PARMG) or ethanol extract (EARMG) of ARMG (125, 250 or 500 mg/kg) or aspirin (30 mg/kg, a reference control) for 3 weeks. Forty min after the final administration, carotid arterial thrombosis was induced by applying a 70% FeCl3-soaked filter paper outside the arterial wall for 5 min, and the blood flow was monitored with a laser Doppler probe. Both PARMG and EARMG delayed the FeCl3-induced arterial occlusion in a dose-dependent manner, doubling the occlusion time at high doses. In mechanism studies, a high concentration of EARMG inhibited platelet aggregation induced by collagen in vitro. In addition, EARMG improved the blood lipid profiles, decreasing triglyceride and cholesterol levels. Although additional action mechanisms remain to be clarified, it is suggested that ARMG containing high amount of ginsenosides such as Rg3 improves blood flow not only by inhibiting oxidative thrombosis, but also by modifying blood lipid profiles.

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Morusinol Extracted from <i>Morus Alba</i> Inhibits Arterial Thrombosis and Modulates Platelet Activation for the Treatment of Cardiovascular Disease

Cited by 37 publications

References 26 publications

Antiplatelet Activity of Morus alba Leaves Extract, Mediated via Inhibiting Granule Secretion and Blocking the Phosphorylation of Extracellular‐Signal‐Regulated Kinase and Akt

Antiplatelet Activity of Morus alba Leaves Extract, Mediated via Inhibiting Granule Secretion and Blocking the Phosphorylation of Extracellular‐Signal‐Regulated Kinase and Akt

Nattokinase improves blood flow by inhibiting platelet aggregation and thrombus formation

Blood flow-improving activity of methyl jasmonate-treated adventitious roots of mountain ginseng

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